Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Bae, Ji‐Hoon; Jang, Kwang-Ho; Park, Seung-Chun; Jin, Hee Kyung

doi:10.1292/jvms.67.111

Cited by 36 publications

(25 citation statements)

References 10 publications

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“…However, the direct effect of acemannan on epithelial proliferation and differentiation could not be excluded. Many plant polysaccharides such as Phellinus gilvus and Opuntia ficus-indica have been reported to have the capacity to enhance wound healing (4,5). We conclude that acemannan accelerated the proliferative and extracellular matrix synthesis phase of oral wound healing via proliferation and upregulation of KGF-1, VEGF, and type I collagen expression in gingival fibroblasts.…”

Section: Discussionmentioning

confidence: 68%

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Acemannan Stimulates Gingival Fibroblast Proliferation; Expressions of Keratinocyte Growth Factor-1, Vascular Endothelial Growth Factor, and Type I Collagen; and Wound Healing

et al. 2009

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Abstract. Aloe vera has long been used as a traditional medicine for inducing wound healing. Gingival fibroblasts (GFs) play an important role in oral wound healing. In this study, we investigated the effects of acemannan, a polysaccharide extracted from Aloe vera gel, on GF proliferation; keratinocyte growth factor-1 (KGF-1), vascular endothelial growth factor (VEGF), and type I collagen production; and oral wound healing in rats. [ 3 H]-Thymidine incorporation assay and ELISA were used. Punch biopsy wounds were created at the hard palate of male Sprague Dawley rats. All treatments (normal saline; 0.1% triamcinolone acetonide; plain 1% Carbopol ® ; and Carbopol ® containing 0.5%, 1%, and 2% acemannan (w /w)) were applied daily. Wounded areas and histological features were observed at day 7 after treatment. From our studies, acemannan at concentrations of 2, 4, 8, and 16 mg/ml significantly induced cell proliferation (P<0.05). Acemannan concentrations between 2 -16 mg/ml significantly stimulated KGF-1, VEGF, and type I collagen expressions (P<0.05). Wound healing of animals receiving Carbopol ® containing 0.5% acemannan (w /w) was significantly better than that of the other groups (P<0.05). These findings suggest that acemannan plays a significant role in the oral wound healing process via the induction of fibroblast proliferation and stimulation of KGF-1, VEGF, and type I collagen expressions.

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Section: Discussionmentioning

confidence: 68%

“…Several herbal and natural products have been investigated for the promotion of wound healing (4,5). Aloe vera (Aloe barbadensis MILLER) has long been used as an herbal medicine for healing skin wounds (6).…”

Section: Introductionmentioning

confidence: 99%

Acemannan Stimulates Gingival Fibroblast Proliferation; Expressions of Keratinocyte Growth Factor-1, Vascular Endothelial Growth Factor, and Type I Collagen; and Wound Healing

et al. 2009

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“…This was done to prevent experimental bias consecutive to animal body movement (Bae et al, 2005). Specific treatments were as follows: Madecassol® cream 1% at the dose of 0.5g (MAD group), Pistacia Lentiscus virgin fatty oil 1ml (PLVFO group), Vaseline gel at the dose of 0.5g (VAS group) and no treatment representing the control group (CRL group).…”

Section: Treatment and Assessment Of Healing Processmentioning

confidence: 99%

Effect of virgin fatty oil of <i>Pistacia lentiscus</i> on experimental burn wound’s healing in rabbits.

Djerrou¹,

Maameri²,

Hamdi-Pacha³

et al. 2010

Afr. J. Trad. Compl. Alt. Med.

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This study aimed to assess the efficiency of the virgin fatty oil of Pistacia lentiscus (PLVFO) for burn wounds healing. It was carried out on 6 adult male New Zealand rabbits. Four burn wounds of deep third degree were made on the back of each animal. The first was not treated and served as control (CRL group); the others were covered immediately after burning procedure by 0.5g of one of the following products: Vaseline gel (VAS group), Madecassol ® cream 1% (MAD group) or 1ml of PLVFO (PLVFO group). The treatments were repeated once daily until complete healing. For four days post burns, the percentage of wound contraction was assessed. Also, the different healing times were noted. The results showed that both PLVFO and Madecassol ® significantly accelerated wound healing activity compared to wounds dressed with Vaseline and the untreated wounds. However, the level of wound contraction was significantly higher and the healing time was faster in PLVFO group than those of the MAD group, VAS group and CRL group. The different epithelization periods obtained in days were respectively: 30±3.94 (PLVFO group), 33.5±3.78 (MAD group), 34.66±3.88 (VAS group) and 37.16±3.54 (CRL group). We conclude that Pistacia lentiscus virgin fatty oil promotes significantly (p< 0.05) wound contraction and reduces epithelization period in rabbit model.

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“…Phellinus gilvus (PG) is a medicinal mushroom belonging to Hymenochaetaceae basidiomycetes , and has advantages over many Phellinus species due to its short growth period (3 mo), making it cheaper to produce (1)(2)(3). Previous studies in our laboratory and by other investigators have established various biological activities of PG.…”

mentioning

confidence: 99%

“…Previous studies in our laboratory and by other investigators have established various biological activities of PG. Among the reported benefits of PG are inhibition of pulmonary inflammation, prevention of intraperitoneal adhesion under infectious circumstances, promotion of dermal wound healing, anti-platelet aggregation and antitumor activities (1)(2)(3)(4). In a previous study with various solvent extracts of PG, we have demonstrated marked DPPH free radical scavenging and xanthine oxidase (XO)-inhibitory activities of the ethyl acetate extract of PG, along with the enhancement of cellular immunity by hot water extracts of PG and inhibition of thrombin-induced platelet aggregation by chloroform-, methanol-and butanol extracts of PG ( 3 ).…”

mentioning

confidence: 99%

In Vitro Antioxidant and Anti-Inflammatory Activities of Protocatechualdehyde Isolated from Phellinus gilvus

Chang

Gebru

Lee

et al. 2011

J Nutr Sci Vitaminol

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Summary Although various biological activities of Phellinus gilvus (PG) have been reported, the active compounds responsible for these effects are not known. Here, we evaluated the activity of various solvent extracts of PG, and found the ethyl acetate extract (Fd) to be the most active fraction, showing a strong DPPH free radical scavenging activity, and inhibitory effects on LPS-induced nitric oxide (NO) production and COX-2 mRNA expression in RAW264.7 macrophages. Six major compounds were identified from the ethyl acetate extract of PG, and protocatechualdehyde (PCA) was supposed to be the major phenolic compound of PG responsible for its DPPH free radical scavenging activity and its inhibitory effects on LPS-induced NO production in RAW264.7 cells. Further in vitro and in vivo experiments are currently underway to confirm this observation and to investigate the detailed molecular mechanisms involved in the process as well as the biological activities of other fractions of Fd. Key Words Phellinus gilvus , protocatechualdehyde, DPPH, NO, RAW264.7 cellsPhellinus gilvus (PG) is a medicinal mushroom belonging to Hymenochaetaceae basidiomycetes , and has advantages over many Phellinus species due to its short growth period (3 mo), making it cheaper to produce ( 1-3 ). Previous studies in our laboratory and by other investigators have established various biological activities of PG. Among the reported benefits of PG are inhibition of pulmonary inflammation, prevention of intraperitoneal adhesion under infectious circumstances, promotion of dermal wound healing, anti-platelet aggregation and antitumor activities ( 1-4 ). In a previous study with various solvent extracts of PG, we have demonstrated marked DPPH free radical scavenging and xanthine oxidase (XO)-inhibitory activities of the ethyl acetate extract of PG, along with the enhancement of cellular immunity by hot water extracts of PG and inhibition of thrombin-induced platelet aggregation by chloroform-, methanol-and butanol extracts of PG ( 3 ). In the current investigation, we determined the major components of the ethyl acetate extract of PG responsible for its biological activities and further compared the magnitude of the antioxidant/anti-inflammatory activities of components with the various fractional extracts of PG.The fruiting body of PG was provided by Gyeongbuk Agriculture Technology Administration (Daegu, Korea). The preparation of PG fractional extracts was reported earlier ( 3 ). Briefly, fruiting bodies of the mushroom were cut into small pieces and dried at 40-50˚C for 48 h. The pieces were homogenized, extracted with water (1 : 25) at 100˚C for 10 h, and the aqueous phase (Fa) concentrated at 80˚C in a rotary evaporator (Büchi Rotavapor R-114, Switzerland) and the final volume reduced to 1/10 of the initial volume. The Fa was then mixed with 95% ethanol (1 : 3, v/v) and stored at 4˚C overnight. The solution was then centrifuged at 15,000 ϫ g for 30 min, the precipitate dialyzed (1 : 100,000) in water, filtered, centrifuged, re-dissolved and ly...

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Promotion of Dermal Wound Healing by Polysaccharides Isolated from Phellinus gilvus in rats

Cited by 36 publications

References 10 publications

Acemannan Stimulates Gingival Fibroblast Proliferation; Expressions of Keratinocyte Growth Factor-1, Vascular Endothelial Growth Factor, and Type I Collagen; and Wound Healing

Acemannan Stimulates Gingival Fibroblast Proliferation; Expressions of Keratinocyte Growth Factor-1, Vascular Endothelial Growth Factor, and Type I Collagen; and Wound Healing

Effect of virgin fatty oil of <i>Pistacia lentiscus</i> on experimental burn wound’s healing in rabbits.

In Vitro Antioxidant and Anti-Inflammatory Activities of Protocatechualdehyde Isolated from Phellinus gilvus

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