Acemannan Stimulates Gingival Fibroblast Proliferation; Expressions of Keratinocyte Growth Factor-1, Vascular Endothelial Growth Factor, and Type I Collagen; and Wound Healing

Abstract

Abstract. Aloe vera has long been used as a traditional medicine for inducing wound healing. Gingival fibroblasts (GFs) play an important role in oral wound healing. In this study, we investigated the effects of acemannan, a polysaccharide extracted from Aloe vera gel, on GF proliferation; keratinocyte growth factor-1 (KGF-1), vascular endothelial growth factor (VEGF), and type I collagen production; and oral wound healing in rats. [ 3 H]-Thymidine incorporation assay and ELISA were used. Punch biopsy wounds were created at the hard palate of male Sprague Dawley rats. All treatments (normal saline; 0.1% triamcinolone acetonide; plain 1% Carbopol ® ; and Carbopol ® containing 0.5%, 1%, and 2% acemannan (w /w)) were applied daily. Wounded areas and histological features were observed at day 7 after treatment. From our studies, acemannan at concentrations of 2, 4, 8, and 16 mg/ml significantly induced cell proliferation (P<0.05). Acemannan concentrations between 2 -16 mg/ml significantly stimulated KGF-1, VEGF, and type I collagen expressions (P<0.05). Wound healing of animals receiving Carbopol ® containing 0.5% acemannan (w /w) was significantly better than that of the other groups (P<0.05). These findings suggest that acemannan plays a significant role in the oral wound healing process via the induction of fibroblast proliferation and stimulation of KGF-1, VEGF, and type I collagen expressions.

Aloe vera as vehicle to mineral trioxide aggregate: study in bone repair

“…Similarly, Jettanacheawchankit et al 24 found that acemannan plays a significant role in the oral wound healing process via the induction of fibroblast proliferation and stimulation of keratinocyte growth factor-1 (KGF-1), vascular endothelial growth factor (VEGF), and type I collagen expressions. In a clinical trial with 40 patients with oral minor aphthous lesions, Babaee et al 25 observed that Aloe vera 2% oral gel is not only effective in decreasing the recurrent aphthous stomatitis patients' pain score and wound size but also decreases the aphthous wound healing period.…”

Putative Prophylaxes Updated of Placenta Extract and Aloe vera as Biogenic Stimulants

“…Wound healing of animals receiving Carbopol containing 0.5% ACE (w/w) was significantly better than that of the other groups (p<0.05). These findings suggest that ACE plays a significant role in the oral wound healing process via the induction of fibroblast proliferation and stimulation of KGF-1, VEGF, and type I collagen expressions [56] .…”

Therapeutic effect of Aloe vera and silver nanoparticles on acid-induced oral ulcer in gamma-irradiated mice

“…8,9 In addition, AV stimulates PGE2, TGF-β-1 and bFGF production and induces angiogenesis, which play important roles in the maintenance of mucosal integrity and wound healing. 31 Moreover, AV enhances production of kGF-1 and VEGF by g ing ival fibroblasts 32 and maintaines the wound moisturized, promotes epithelial cell migration, and enhances collagen maturation. 33 AgNPs exerts its healing effect by decreasing bacterial and fungal count at wound site.…”