Progenitor Cells From Dental Follicle Are Able to Form Cementum Matrix In Vivo

Handa, Keisuke; Saito, Masahiro; Tsunoda, Akira; Yamauchi, Masato; Hattori, Shintaro; Sato, Sadao; Toyoda, Minoru; Teranaka, Toshio; Narayanan, A. Sampath

doi:10.1080/713713535

Cited by 19 publications

(18 citation statements)

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“…Although previous studies have analyzed DFC from the root surface during root formation, no data exist for cells at the early crownformation stage (Hakki et al 2001;Handa et al 2002;Jin et al 2003;Saito et al 2005). The isolation of progenitor cells from the DF may therefore help to identify the as-yetunknown mechanism for differentiation into the cementoblasts, PDL fibroblasts, and osteoblasts that make up the periodontium (Lekic et al 1996;Pitaru et al 1994).…”

Section: Discussionmentioning

confidence: 96%

“…Progenitor cells isolated from bovine DF at the root-formation stage have previously been shown to generate cementum (Handa et al 2002); however, no data exist for DFC at the early crown-formation stage. Examination of the gene expression patterns related to osteogenesis has revealed the expression of BSP at 2 weeks after transplantation, and OCN, periostin, and collagen type III expressions gradually increase.…”

Section: Discussionmentioning

confidence: 99%

“…To extend the comparison of DFC-I cultured on Col-I-d and on P-d, we investigated the potential for mineralization in vivo by transplanting DFC-I in conjunction with TCP into the subcutaneous tissue of immunocompromised scid mice as described previously (Handa et al 2002;Saito et al 2005). At 1, 2, and 4 weeks after transplantation, we examined the relevant gene expression in the implants by sq-PCR.…”

Section: Developmental Potential Of Clone-dfc-i In Vivomentioning

confidence: 99%

“…The differentiation potential of individual DFC-I on transplantation into immunodeficient mice was assessed as described previously (Handa et al 2002). Briefly, 1.5×10 6 DFC-I were incubated in a mixture of 40 mg β-tricalcium phosphate powder (TCP; Osferion G1, no.…”

Section: In Vivo Differentiation Assaymentioning

confidence: 99%

“…Although the exact sequence of events and cells involved in the development of the periodontium is not established, previous studies have suggested that DFC populations also contain cementoblasts, periodontal ligament cells (PDLC), and osteoblast progenitor or precursor cells (Morsczeck et al 2005;Palmer and Lumsden 1987;Ten Cate and Mills 1972;Ten Cate et al 1971;Yoshikawa and Kollar 1981). Bovine DFC isolated from developing tooth germ at the root-forming stage can differentiate into cementoblasts on implantation into immunodeficient mice (Handa et al 2002). However, the mechanisms regulating DFC differentiation remain poorly understood (Bartold et al In the first part of this study, we isolated porcine DFC from third molars extracted at the early crown-formation stage.…”

Section: Introductionmentioning

confidence: 98%

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Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

et al. 2007

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We investigated porcine dental follicle cells at the early crown-formation stage and examined the behavior of cells grown in a collagen type I (Col-I) matrix. Clone-porcine dental follicle cells (DFC-I) and controls, viz., dental follicle itself, nonclone-dental follicle cells, periodontal ligament cells (PDLC), and bone marrow stromal cells, were obtained from 6-month-old pigs. DFC-I showed a different gene expression pattern from controls by reverse-transcription polymerase chain reaction analysis. In addition, Col-I treatment enhanced DFC-I proliferation and increased their alkaline phosphatase activity compared with nontreated DFC-I. The expression of periostin, biglycan, and osteocalcin (OCN) in cells growing on collagen was upregulated, similar to the pattern seen in PDLC. DFC-I with and without Col-I treatment were combined with beta-tricalcium phosphate particles and implanted into immunodeficient mice. Significant differences were found in the gene expression patterns of bone sialoprotein, OCN, and periostin in both treated and non-treated implants at 2 and/or 4 weeks. The results showed that Col-I induced the mineralization pathway in these cells. Hard tissue formation was observed in both implant types at 8 weeks. Our results suggest that Col-I facilitates the differentiation of DFC-I along the mineralization process.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Developmental Potential Of Clone-dfc-i In Vivomentioning

confidence: 99%

Section: In Vivo Differentiation Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

et al. 2007

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Dental stem cell banking: Techniques and protocols

Khaseb¹,

Orooji

Pour

et al. 2021

Cell Biology International

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Dental tissue‐derived stem cells (DSCs) provide an easy, accessible, relatively noninvasive promising source of adult stem cells (ASCs), which brought encouraging prospective for their clinical applications. DSCs provide a perfect opportunity to apply for a patient's own ASC, which poses a low risk of immune rejection. However, problems associated with the long‐term culture of stem cells, including loss of proliferation and differentiation capacities, senescence, genetic instability, and the possibility of microbial contamination, make cell banking necessary. With the rapid development of advanced cryopreservation technology, various international DSC banks have been established for both research and clinical applications around the world. However, few studies have been published that provide step‐by‐step guidance on DSCs isolation and banking methods. The purpose of this review is to present protocols and technical details for all steps of cryopreserved DSCs, from donor selection, isolation, cryopreservation, to characterization and quality control. Here, the emphasis is on presenting practical principles in accordance with the available valid guidelines.

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Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

Açil

Yang

Gülses³

et al. 2015

Odontology

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The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno-histo-chemistry staining of osteocalcin. Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

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Progenitor Cells From Dental Follicle Are Able to Form Cementum Matrix In Vivo

Cited by 19 publications

References 0 publications

Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

Dental stem cell banking: Techniques and protocols

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

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