Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

Abstract

We investigated porcine dental follicle cells at the early crown-formation stage and examined the behavior of cells grown in a collagen type I (Col-I) matrix. Clone-porcine dental follicle cells (DFC-I) and controls, viz., dental follicle itself, nonclone-dental follicle cells, periodontal ligament cells (PDLC), and bone marrow stromal cells, were obtained from 6-month-old pigs. DFC-I showed a different gene expression pattern from controls by reverse-transcription polymerase chain reaction analysis. In addition, Col-I treatment enhanced DFC-I proliferation and increased their alkaline phosphatase activity compared with nontreated DFC-I. The expression of periostin, biglycan, and osteocalcin (OCN) in cells growing on collagen was upregulated, similar to the pattern seen in PDLC. DFC-I with and without Col-I treatment were combined with beta-tricalcium phosphate particles and implanted into immunodeficient mice. Significant differences were found in the gene expression patterns of bone sialoprotein, OCN, and periostin in both treated and non-treated implants at 2 and/or 4 weeks. The results showed that Col-I induced the mineralization pathway in these cells. Hard tissue formation was observed in both implant types at 8 weeks. Our results suggest that Col-I facilitates the differentiation of DFC-I along the mineralization process.

Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder

“…However, further studies are necessary to clarify the exact nature of this effect by specific amino acids and specific molecular weight fractions in the protein. Type I collagen has been reported to facilitate the differentiation of clonal porcine dental follicle cells and bone marrow cells [9][10][11] . However, the collagen used in those studies was mammalian.…”

Osteogenic Differentiation Capacity of Porcine Dental Follicle Progenitor Cells

“…We previously isolated porcine dental follicle cells in the early stages of tooth crown formation. Clonal populations of these cells could be differentiated into periodontal ligament cells on collagen type I matrix in vitro and had the capacity to generate either cementum or bone in vivo in combination with β-tricalcium phosphate powder (TCP) particles in subcutaneous space [20]. The osteogenic differentiation capacity of such progenitor cells in vivo, in combination with TCP particles in subcutaneous space, was also shown by other studies [14,21,22].…”

“…A cloned dental follicle progenitor (DFC-I) cell line was developed from porcine third molar teeth at the crown formation stage, as described previously [20]. Briefly, dental follicle tissue was separated from the dental epithelium of porcine third molar teeth.…”

Dental stem cells for tooth regeneration and repair

“…In a clone of porcine dental follicle cells, after transplanta tion experiments, differences in the expression of BSP and periostin were found under the influence of collagen 1, which facilitated the mineralization process [45]. An under standing of the mechanisms that controls the pathways involved in dental follicle cell differentiation, which, in turn, will result in a mineralised or a fibroblastic cell type (bone or periodontal ligament), imply that in the future, the pro curement of periodontal ligament cells will not necessarily require an extracted tooth.…”