Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder

Yamada, Sōichiro; Yoshizawa, Yuu; Kawakubo, Atsushi; Ikeda, Takeshi; Yanagiguchi, Kajiro; Hayashi, Yoshihiko

doi:10.4012/dmj.2012-188

Cited by 30 publications

(28 citation statements)

References 28 publications

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“…Incubation of human osteoblasts with 0.1% fish HC increased osteocalcin, osteopontin, BMP-2 and integrin β3 mRNA expression and accelerated matrix mineralization as compared to untreated cells (Yamada, Yoshizawa, et al, 2013). Consequently, this translated into increased calcium disposal or mineralization in either human or murine osteoblasts (Liu et al, 2014;Tsuruoka et al, 2007;Yamada, Nagaoka, et al, 2013;Yamada, Yoshizawa, et al, 2013) (Fig. 5).…”

Section: Mechanisms Involved In Collagen Effects On Bonementioning

confidence: 87%

“…Additionally, HC-induction of the bone-specific transcription factor osterix was associated with the up-reglation of type I collagen expression, thus providing insights into the molecular basis of HC action on osteoblasts (H. K. Kim et al, 2014a;Tsuruoka et al, 2007;Yamada, Yoshizawa, et al, 2013). Bovine HC was shown to stimulate osteoblast differentiation, mineralized bone matrix formation, ALP activity, and osteocalcin production through increased Kim et al, 2013).…”

Section: Molecular Mechanismsmentioning

confidence: 93%

“…Therefore testing the effect of serum from animals fed specific enriched diets on cellular outcomes should provide better information for evaluation of dietary effect on specific organ. It should be noted that only Tsuruoka et al (Tsuruoka et al, 2007) Tsuruoka et al, 2007;Yamada, Yoshizawa, et al, 2013) as well as alkaline phosphatase activity (Guillerminet et al, 2010;H. K. Kim et al, 2013;Yamada, Nagaoka, et al, 2013).…”

Section: Mechanisms Involved In Collagen Effects On Bonementioning

confidence: 95%

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Biological Effect of Hydrolyzed Collagen on Bone Metabolism

Daneault

Coxam

Wittrant

2015

Critical Reviews in Food Science and Nutrition

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Osteoporosis is a chronic and asymptomatic disease characterized by low bone mass and skeletal microarchitectural deterioration, increased risk of fracture, and associated comorbidities most prevalent in the elderly. Due to an increasingly aging population, osteoporosis has become a major health issue requiring innovative disease management. Proteins are important for bone by providing building blocks and by exerting specific regulatory function. This is why adequate protein intake plays a considerable role in both bone development and bone maintenance. More specifically, since an increase in the overall metabolism of collagen can lead to severe dysfunctions and a more fragile bone matrix and because orally administered collagen can be digested in the gut, cross the intestinal barrier, enter the circulation, and become available for metabolic processes in the target tissues, one may speculate that a collagen-enriched diet provides benefits for the skeleton. Collagen-derived products such as gelatin or hydrolyzed collagen (HC) are well acknowledged for their safety from a nutritional point of view; however, what is their impact on bone biology? In this manuscript, we critically review the evidence from literature for an effect of HC on bone tissues in order to determine whether HC may represent a relevant alternative in the design of future nutritional approaches to manage osteoporosis prevention.

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Section: Mechanisms Involved In Collagen Effects On Bonementioning

confidence: 87%

Section: Molecular Mechanismsmentioning

confidence: 93%

Section: Mechanisms Involved In Collagen Effects On Bonementioning

confidence: 95%

See 1 more Smart Citation

Biological Effect of Hydrolyzed Collagen on Bone Metabolism

Daneault

Coxam

Wittrant

2015

Critical Reviews in Food Science and Nutrition

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“…Including an appropriate functional scaffold (i.e., intricate 3D mesh composed of salmon collagen-coated fibers) may potentially improve the osteogenic potential of cultured periosteal sheets as a graft biomaterial both in vitro and in vivo [104]. Furthermore, fish collagen peptides promote posttranscriptional modification for collagen maturation and the gene expression for cell differentiation in osteoblastic cells [105, 106]. These findings indicate that fish collagen application in situ may promote hard tissue formation as both a scaffold for seeded cells and a nutritional factor for growth.…”

Section: Application For Use As a Biomaterialsmentioning

confidence: 99%

Potency of Fish Collagen as a Scaffold for Regenerative Medicine

Yamada

Yamamoto

Ikeda

et al. 2014

BioMed Research International

Self Cite

156

114

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Cells, growth factors, and scaffold are the crucial factors for tissue engineering. Recently, scaffolds consisting of natural polymers, such as collagen and gelatin, bioabsorbable synthetic polymers, such as polylactic acid and polyglycolic acid, and inorganic materials, such as hydroxyapatite, as well as composite materials have been rapidly developed. In particular, collagen is the most promising material for tissue engineering due to its biocompatibility and biodegradability. Collagen contains specific cell adhesion domains, including the arginine-glycine-aspartic acid (RGD) motif. After the integrin receptor on the cell surface binds to the RGD motif on the collagen molecule, cell adhesion is actively induced. This interaction contributes to the promotion of cell growth and differentiation and the regulation of various cell functions. However, it is difficult to use a pure collagen scaffold as a tissue engineering material due to its low mechanical strength. In order to make up for this disadvantage, collagen scaffolds are often modified using a cross-linker, such as gamma irradiation and carbodiimide. Taking into account the possibility of zoonosis, a variety of recent reports have been documented using fish collagen scaffolds. We herein review the potency of fish collagen scaffolds as well as associated problems to be addressed for use in regenerative medicine.

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“…The expression levels of DSPP and OCN mRNA were determined by SYBR green real-time reverse transcription-PCR as described previously 19,20) . DPCs were infected with AAV2-BMP-7 or AAV2-EGFP and were cultured for 7, 14, or 21 days.…”

Section: The Expression Of Dspp and Ocn Genesmentioning

confidence: 99%

Dental pulp cells that express adeno-associated virus serotype 2-mediated BMP-7 gene enhanced odontoblastic differentiation

et al. 2014

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This present study investigated the potential of adeno-associated virus serotype 2 (AAV2) mediated BMP-7 (AAV2-BMP-7) to induce odontoblastic differentiation of human dental pulp cells (DPCs) in vitro. AAV2-BMP-7 was constructed to overexpress BMP-7, and the biologic effects of BMP-7 on DPCs were investigated by the evaluation of the activity of alkaline phosphatase (ALPase), the detection of the expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN) expression and the analysis of the proliferative ability of the cells. DPCs that were infected with AAV2-BMP-7 displayed significantly upregulated ALP activity and formed mineralized nodules. Moreover, AAV2-BMP-7 promoted the expression of mineralization-related genes, which included DSPP and OCN. In addition, there was no significant difference between the proliferative ability of AAV2-BMP-7 and the control group. In conclusion, AAV2-BMP-7 promoted the odontoblastic differentiation in DPCs, a clear indication of the therapeutic potential of AAV2-BMP-7 in dental tissue regeneration.

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Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder

Cited by 30 publications

References 28 publications

Biological Effect of Hydrolyzed Collagen on Bone Metabolism

Biological Effect of Hydrolyzed Collagen on Bone Metabolism

Potency of Fish Collagen as a Scaffold for Regenerative Medicine

Dental pulp cells that express adeno-associated virus serotype 2-mediated BMP-7 gene enhanced odontoblastic differentiation

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