Dental pulp cells that express adeno-associated virus serotype 2-mediated BMP-7 gene enhanced odontoblastic differentiation

Qin, Wei; Zhu, Haiyan; Yang, Xiaoting; Huang, Qiting; Zhao, Lin

doi:10.4012/dmj.2014-109

Cited by 2 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results still tie well with the previous studies, wherein the adenovirus overexpressed BMP7 in DPCs upregulated the odontoblastic differentiation and maintained an equal proliferative ability to the control group. 57 …”

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein 7 mediates stem cells migration and angiogenesis: therapeutic potential for endogenous pulp regeneration

Cheng

Liang

et al. 2022

Int J Oral Sci

View full text Add to dashboard Cite

Pulp loss is accompanied by the functional impairment of defense, sensory, and nutrition supply. The approach based on endogenous stem cells is a potential strategy for pulp regeneration. However, endogenous stem cell sources, exogenous regenerative signals, and neovascularization are major difficulties for pulp regeneration based on endogenous stem cells. Therefore, the purpose of our research is to seek an effective cytokines delivery strategy and bioactive materials to reestablish an ideal regenerative microenvironment for pulp regeneration. In in vitro study, we investigated the effects of Wnt3a, transforming growth factor-beta 1, and bone morphogenetic protein 7 (BMP7) on human dental pulp stem cells (h-DPSCs) and human umbilical vein endothelial cells. 2D and 3D culture systems based on collagen gel, matrigel, and gelatin methacryloyl were fabricated to evaluate the morphology and viability of h-DPSCs. In in vivo study, an ectopic nude mouse model and an in situ beagle dog model were established to investigate the possibility of pulp regeneration by implanting collagen gel loading BMP7. We concluded that BMP7 promoted the migration and odontogenic differentiation of h-DPSCs and vessel formation. Collagen gel maintained the cell adhesion, cell spreading, and cell viability of h-DPSCs in 2D or 3D culture. The transplantation of collagen gel loading BMP7 induced vascularized pulp-like tissue regeneration in vivo. The injectable approach based on collagen gel loading BMP7 might exert promising therapeutic application in endogenous pulp regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Bone morphogenetic protein 7 mediates stem cells migration and angiogenesis: therapeutic potential for endogenous pulp regeneration

Cheng

Liang

et al. 2022

Int J Oral Sci

View full text Add to dashboard Cite

show abstract

The Efficiency of rhBMP-7 in Oral and Maxillofacial Bone Defects: A Systematic Review

2017

J. Diagn. Treat. Oral Maxillofac. Pathol.

View full text Add to dashboard Cite

Background. Bone morphogenic protein-7 (BMP-7) is a molecule that has been clinically trialed and tested for use in regeneration of bone defects and its ability to induce bone formation by induction of gene expression. Recombinant human BMP-7 (rhBMP-7) has surgical uses and is marketed under the brand name OP-1 (Olympus Biotech Corporation). With the ever expanding discipline of oral surgery and the need for regenerative therapy for bone defects in the oral and maxillofacial region, this study was undertaken to compare the outcomes of different methods of use of BMP-7. Methods. The authors retrieved English publications on relevant studies from PubMed, ScienceDirect and Google Scholar from 2000 to 2016. In vitro and in vivo studies were included. The main outcomes were increased expression of osteogenic genes and radiographic bone fill. Results. In total, 676 human studies in the literature were identified but only four were ultimately feasible. The results showed a significant effect of rhBMP-7 both in-vitro and in-vivo. In all the studies used a systematic review was used to compare the outcomes of using rhBMP-7. The in vivo samples showed significant radiographic fill and efficient bone deposition [4]. In vitro studies showed increased expression of OCN and ALP biomarkers. Conclusions. rhBMP-7 is an effective tool that can be used to improve and speed up regenerative therapy of bone defects. However further human studies are needed to enhance and develop the use of this molecule in vitro.

show abstract

Dental pulp cells that express adeno-associated virus serotype 2-mediated BMP-7 gene enhanced odontoblastic differentiation

Cited by 2 publications

References 36 publications

Bone morphogenetic protein 7 mediates stem cells migration and angiogenesis: therapeutic potential for endogenous pulp regeneration

Bone morphogenetic protein 7 mediates stem cells migration and angiogenesis: therapeutic potential for endogenous pulp regeneration

The Efficiency of rhBMP-7 in Oral and Maxillofacial Bone Defects: A Systematic Review

Contact Info

Product

Resources

About