Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

Abstract

The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno-histo-chemistry staining of osteocalcin. Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

A comparative study on immunophenotypic characterization and osteogenic differentiation of human mesenchymal stromal cells derived from periodontal ligament and gingiva

“…29 Yahya Acil et al also detected PDL cells were spindle-shaped and fibroblast-like. 30 Yuan Gao et al reported that the morphological characteristics of DSCs, GMSCs, and PDLSCs were similar. 10 In this current study, PDLMSCs and GMSCs met four criteria for being MSCs: (1) PDLMSCs and GMSCs adhered in cell culture flask after 24 hours (Figure 1).…”

Mesenchymal Stem Cells for Optimizing Bone Volume at the Dental Implant Recipient Site

“…Briefly [31], impacted third molars were surgically extracted, and periodontal dental ligament was gently scraped from the middle root surface. Coronal and apical portions of the ligament were not used in order to avoid contamination by gingival and pulpal cells.…”

“…After digestion, tissue was seeded into culture flasks with alpha-modification of Eagle's Medium supplemented with 10% fetal bovine serum, 2-mM Glutamine, 100-U/mL penicillin and 100-μg/mL: streptomycin solution at 37°C in 5% CO 2 in a humidified atmosphere. After single cells were attached on the plastic bottom of the flask, non-adherent cells were removed by changing the medium [31].…”

Interleukin‐12 modulates the immunomodulatory properties of human periodontal ligament cells

“…These cells remodel the extracellular matrix (ECM), of which collagen fibers are the most prominent structures. PDL cells have been proposed as a source of mesenchymal stem cells (MSCs) . PDL cells possess characteristics of other stem cells, including: (i) a high level of MSC markers such as CD73, CD90 and CD105; (ii) self‐renewal capacity; and (iii) multilineage differentiation potential .…”

“…PDL cells have been proposed as a source of mesenchymal stem cells (MSCs) . PDL cells possess characteristics of other stem cells, including: (i) a high level of MSC markers such as CD73, CD90 and CD105; (ii) self‐renewal capacity; and (iii) multilineage differentiation potential . Among tooth‐derived MSCs, PDL cells have been demonstrated to have a very high capacity to differentiate into mineral‐secreting cells in the presence of osteoinductive factors .…”