A comparative study on immunophenotypic characterization and osteogenic differentiation of human mesenchymal stromal cells derived from periodontal ligament and gingiva

Abedian, Zeinab; Jenabian, Niloofar; Moghadamnia, Ali Akbar; Zabihi, Ebrahim; Pourbagher, Roghayeh; Hossein-Nataj, Hadi; Mohamadnia-Afrouzi, Mousa

doi:10.1002/jper.19-0535

Cited by 24 publications

(19 citation statements)

References 31 publications

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“…The experimental protocol was approved by the institutional review board of the Dankook University Dental Hospital, Cheonan, Korea (approval number: DKUDH IRB 2019-01-006). Human PDLSCs were isolated as described in detail in a previous report [ 41 ]. Briefly, periodontal ligament (PDL) tissue was carefully dissected from the middle third of the root with a sterilized scalpel and minced into pieces that were as small as possible.…”

Section: Methodsmentioning

confidence: 99%

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Kim

Lee

et al. 2020

Nanomaterials

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Calcium silicate-based bioactive glass has received significant attention for use in various biomedical applications due to its excellent bioactivity and biocompatibility. However, the bioactivity of calcium silicate nanoparticle-incorporated bioactive dental sealer is not much explored. Herein, three commercially available bioactive root canal sealers (Endoseal MTA (EDS), Well-Root ST (WST), and Nishika Canal Sealer BG (NBG)) were compared with a resin-based control sealer (AH Plus (AHP)) in terms of physical, chemical, and biological properties. EDS and NBG showed 200 to 400 nm and 100 to 200 nm nanoparticle incorporation in the SEM image, respectively, and WST and NBG showed mineral deposition in Hank’s balanced salt solution after 28 days. The flowability and film thickness of all products met the ISO 3107 standard. Water contact angle, linear dimensional changes, and calcium and silicate ion release were significantly different among groups. All bioactive root canal sealers released calcium ions, while NBG released ~10 times more silicon ions than the other bioactive root canal sealers. Under the cytocompatible extraction range, NBG showed prominent cytocompatibility, osteogenecity, and angiogenecity compared to other sealers in vitro. These results indicate that calcium silicate nanoparticle incorporation in dental sealers could be a potential strategy for dental periapical tissue regeneration.

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Section: Methodsmentioning

confidence: 99%

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Kim

Lee

et al. 2020

Nanomaterials

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“…Meanwhile, they found that GMSCs display stable morphology and do not lose MSC characteristics and maintain telomerase activity following long-term cultures (22). In comparison to other types of dental MSCs, previous studies indicated that GMSCs possess less osteogenic potentials than PDLSCs (27,32,33). However, compared to PDLSCs, GMSCs are less susceptible to replicative senescence and proinflammatory cytokine-induced impairment in osteogenic potentials in vitro and ectopic bone formation in vivo (27).…”

Section: Isolation and Characterization Of Human Gingiva-derived Mesenchymal Stem Cellsmentioning

confidence: 99%

Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review

Kim

Lee

et al. 2021

Front. Immunol.

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A unique subpopulation of mesenchymal stem cells (MSCs) has been isolated and characterized from human gingival tissues (GMSCs). Similar to MSCs derived from other sources of tissues, e.g. bone marrow, adipose or umbilical cord, GMSCs also possess multipotent differentiation capacities and potent immunomodulatory effects on both innate and adaptive immune cells through the secretion of various types of bioactive factors with immunosuppressive and anti-inflammatory functions. Uniquely, GMSCs are highly proliferative and have the propensity to differentiate into neural cell lineages due to the neural crest-origin. These properties have endowed GMSCs with potent regenerative and therapeutic potentials in various preclinical models of human disorders, particularly, some inflammatory and autoimmune diseases, skin diseases, oral and maxillofacial disorders, and peripheral nerve injuries. All types of cells release extracellular vesicles (EVs), including exosomes, that play critical roles in cell-cell communication through their cargos containing a variety of bioactive molecules, such as proteins, nucleic acids, and lipids. Like EVs released by other sources of MSCs, GMSC-derived EVs have been shown to possess similar biological functions and therapeutic effects on several preclinical diseases models as GMSCs, thus representing a promising cell-free platform for regenerative therapy. Taken together, due to the easily accessibility and less morbidity of harvesting gingival tissues as well as the potent immunomodulatory and anti-inflammatory functions, GMSCs represent a unique source of MSCs of a neural crest-origin for potential application in tissue engineering and regenerative therapy.

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“…A total of 26 studies were selected for the current systematic review. Of these, 20 in vitro were culture studies [44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63] and 6 were in vivo studies [64][65][66][67][68][69]. All papers were published in the English language, and the date of publication started in 2009.…”

Section: Characteristics Of In Vitro Included Studiesmentioning

confidence: 99%

The Gingiva from the Tissue Surrounding the Bone to the Tissue Regenerating the Bone: A Systematic Review of the Osteogenic Capacity of Gingival Mesenchymal Stem Cells in Preclinical Studies

Al-Qadhi

Aboushady

Al-Sharabi

2021

Stem Cells International

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The current review aims to systematically assess the osteogenic capacity of gingiva-derived mesenchymal stem cells (GMSCs) in preclinical studies. A comprehensive electronic search of PubMed, Embase, Web of Science, and Scopus databases, as well as a manual search of relevant references, was performed in June 2020 without date or language restrictions. Eligibility criteria were the following: studies that compared mesenchymal stem cells (MSCs) derived from the gingiva with other MSC sources (in vitro or in vivo) or cell-free scaffold (in vivo) and studies that reported at least one of the following outcomes: osteogenic potential and new bone formation for in vitro and in vivo, respectively. Moreover, the assessment of included studies was conducted using appropriate guidelines. From 646 initial retrieved studies, 35 full-text articles were subjected to further screening and 26 studies were selected (20 in vitro studies and 6 in vivo studies). GMSCs showed great proliferation capacity and expressed recognized mesenchymal stem cell markers, particularly CD90. In vitro, MSC sources including GMSCs were capable of undergoing osteogenic differentiation with less ability in GMSCs, while most in vivo studies confirmed the capacity of GMSCs to regenerate bony defects. Concerning the assessment of methodological quality, in vitro studies met the relevant guideline except in five areas: the sample size calculation, randomization, allocation concealment, implementation, and blinding, and in vivo publications had probably low risk of bias in most domains except in three areas: allocation concealment, attrition, and blinding items.

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A comparative study on immunophenotypic characterization and osteogenic differentiation of human mesenchymal stromal cells derived from periodontal ligament and gingiva

Cited by 24 publications

References 31 publications

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review

The Gingiva from the Tissue Surrounding the Bone to the Tissue Regenerating the Bone: A Systematic Review of the Osteogenic Capacity of Gingival Mesenchymal Stem Cells in Preclinical Studies

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