Dental stem cell banking: Techniques and protocols

Abstract

Dental tissue‐derived stem cells (DSCs) provide an easy, accessible, relatively noninvasive promising source of adult stem cells (ASCs), which brought encouraging prospective for their clinical applications. DSCs provide a perfect opportunity to apply for a patient's own ASC, which poses a low risk of immune rejection. However, problems associated with the long‐term culture of stem cells, including loss of proliferation and differentiation capacities, senescence, genetic instability, and the possibility of microbial contamination, make cell banking necessary. With the rapid development of advanced cryopreservation technology, various international DSC banks have been established for both research and clinical applications around the world. However, few studies have been published that provide step‐by‐step guidance on DSCs isolation and banking methods. The purpose of this review is to present protocols and technical details for all steps of cryopreserved DSCs, from donor selection, isolation, cryopreservation, to characterization and quality control. Here, the emphasis is on presenting practical principles in accordance with the available valid guidelines.

Identification of potentially functional circRNAs and prediction of circRNA‐miRNA‐mRNA regulatory network in periodontitis: Bridging the gap between bioinformatics and clinical needs

“…Approaches such as normalization addressing enrichment will help reduce false positives 65,66 . Standard tissue sample banks would provide a tremendous opportunity for future studies 67 …”

Advances in Regenerative Dentistry Approaches: An Update

“…However, long-term in vitro culture may pose potential hazards such as chromosomal abnormality, senescence, and microbial contamination. 89 , 90 , 91 Cell banking is used to avoid these risks and to preserve the cells at their most potent stage for future applications. The quality control and maintenance of this multistep process is carried out and kept following international guidelines to ensure its safety and effectiveness.…”

“…The quality control and maintenance of this multistep process is carried out and kept following international guidelines to ensure its safety and effectiveness. 91 …”

Mesenchymal Stem Cells Based Treatment in Dental Medicine: A Narrative Review

“…A broad variety of protocols regarding the collection of stem cells exists. The protocols and technical details of the entire process of stem cell banking are thoroughly described in a recent review written by Khaseb et al [73]. However, regardless of the method by which the sample was obtained, storage for transport has a key role in cell survival [72].…”