Profil Histopatologi Otot Jantung pada Tikus Hipertensi (Doca-Garam) yang Dipapar Kombinasi Ekstrak Metanolik Benalu Teh Dan Benalu Mangga (EMBTBM)

Hakimah, Annisatul Anis; Sjakoer, Nour Athiroh Abdoes; Mubarakati, Nurul Jadid

doi:10.24843/metamorfosa.2021.v08.i02.p20

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…This indicates that damage has occurred to cells and tissues. This is in accordance with research by Hakimah et al (2021), that hypertension can change the structure and tissue of cells, so that it can potentially lead to a fatal condition, namely cell death (Hakimah et al, 2021). The test group for ethanol extract of pomelo orange peel which did not have changes in macroscopic morphology was the 100 mg/kg BW dose group.…”

Section: Resultssupporting

confidence: 87%

Effects of Pamelo Orange Peel (Citrus maxima) Ethanol Extract on Macroscopic Kidney and Heart of Hypertensive Rats

Arifin,

Emelda,

Santi

et al. 2024

JFG

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Background: Hypertension, characterized by elevated blood pressure (≥140/90 mmHg), leads to kidney and heart damage. Hypertension causes increased heart rate, heart enlargement, risk of heart failure, and damage to kidney blood vessels. One way to treat hypertension is by administering drugs such as herbal medicines. Pamelo oranges (Citrus maxima) are starting to be developed in the treatment of diseases. The ethanol extract of pomelo orange peel (EEKJP) contains flavonoid compounds which have antihypertensive activity as a natural Angiotensin Converting Enzyme (ACE) inhibitor, diuretic, increases Nitric Oxide Synthase (NOS) activity and activates Endothelium Derived Relaxing Factor (EDRF). Objectives: This study aims to examine the effect of ethanol extract of pomelo orange peel on the macroscopic features of the heart and kidneys of hypertensive rats (Rattus norvegicus). Methods: This research uses The Posttest-Only Control Group Design. Thirty rats were divided into six groups, namely naive control, negative control (NaCMC 1%), positive control (Captopril 0,45 mg/200 gramBB), EEKJP 100 mg/KgBW group, EEKJP 150 mg/KgBW group, and EEKJP 200 mg/KgBW group. Hypertension condition through induction with prednisone 1.5 mg/KgBW and NaCl 2% for 21th days. The test preparation was administered orally for 14th days. Next, all rats were dissected to remove the heart and kidney organs for macroscopic observations. Results: The results of the study showed that ethanol extract of pomelo (Citrus maxima) orange peel had a varied effect on the macroscopic appearance of the heart and kidneys of rats with hypertension. The heart organ shows structural improvements, while the kidneys have not shown any improvement. Conclusions: Extract with a dose of 100 mg/KgBW has no changes in the macroscopic appearance of the heart so it is thought to be able to repair damage to the heart organ.

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Section: Resultssupporting

confidence: 87%

Effects of Pamelo Orange Peel (Citrus maxima) Ethanol Extract on Macroscopic Kidney and Heart of Hypertensive Rats

Arifin,

Emelda,

Santi

et al. 2024

JFG

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“…Hasil pengamatan gambaran histologi miokardium jantung terlihat perubahan struktur histologi miokardium jantung yang ditandai dengan adanya inti sel piknotik pada histologi jantung dengan karakteristik inti sel semakin kearah tepi sel, inti sel juga menunjukkan kondisi memadat (membengkak), mengkerut, anak inti(nukleolus) tidak jelas atau tidak terlihat dan berwarna lebih basa [17]. Inti sel piknotik yang terdapat pada gambaran histologi miokardium jantung merupakan tahap awal dari kerusakan sel.…”

Section: Hasil Dan Pembahasanunclassified

Pengaruh Ekstrak Etanol Daun Asam Jawa (Tamarindus indica L.) terhadap Jantung Tikus Putih (Rattus norvegicus L.) yang Diinduksi Minyak Jelantah

2023

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This study aims to determine the effect of ethanol tamarind leaves (Tamarindus indica L.) om histology and levels of the enzyme lactate dehydrogenase (LDH) in rat hearts induced by used cooking oil.This study used a completely randomized design with 25 male white rats devided into 5 groups: negetive control, positive control, 1, 2 and 3 doses treatment (150 mg/kg BW, 200 mg/kg BW, and 250 mg/kg BW), where each group consisted of 5 replications. The stages of this research were phytochemical screening, total flavonoid test, antioxidant test, oil quality test, measurement of lactate dehydrogenase (LDH) enzyme levels and cardiac histological observations. The data analysis used was one way ANOVA followed by Duncan’s test. The results showed that the dose of ethanol extract of tamarind leaves (Tamarindus indica L.) 250 mg/kg BW was the most optimal dose to reduce histological damage in the form of pycnotic percentage with an average of 30.40% and thickness of the myocardium of the heart with an average of 852.8 um and decreased cardiac lactate dehydrogenase (LDH) levels by an average of 152 U/L. The ethanol extract of tamarind leaves (Tamarindus indica L.) had an effect on the hearts of white rats (Rattus norvegicus L.) induced by used cooking oil.

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Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) yang Diinduksi Insulin.

Tana

Shivaluhung

Suprihatin

2022

BAF

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Prevalensi prediabetes meningkat setiap tahunnya dan diprediksi lebih dari 470 juta orang mengalami prediabetes pada tahun 2030. Induksi insulin eksogen secara terus-menerus menyebabkan resistensi insulin yang berujung pada kenaikan kadar glukosa darah yang dapat berefek pada histologi ren. Penelitian ini bertujuan untuk mengetahui gambaran histologi ren yang meliputi bobot ren, tebal kapsula Bowman, ukuran sel tubulus proksimal, ukuran sel tubulus distal, dan indeks ren setelah diinduksi dengan insulin eksogen. Penelitian ini dilaksanakan selama 6 bulan di Laboratorium Biologi Sktruktur dan Fungsi Hewan dan di kandang hewan uji, Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro. Penelitian ini menggunakan 21 ekor tikus putih yang dibagi menjadi 3 kelompok perlakuan dan 7 ulangan. P0 (kontrol), P1 (diiduksi insulin glargine 1,80 IU/200gBB/hari selama 14 hari), dan P2 (diinduksi insulin glargine 1,80 IU/200gBB/hari selama 28 hari). Insulin glargine diinjeksikan melalui subkutan dengan menggunakan xiulin pen. Preparat histologi ren dibuat dengan metode parafin dan pewarnaan Hematoksilin-Eosin. Pengamatan preparat menggunakan alat fotomikrograf. Data penelitian kemudian dianalisis dengan uji Anova. Hasil uji Anova menunjukkan antar perlakuan berbeda tidak nyata. Kesimpulan dari penelitian ini adalah induksi insulin 1,80 IU/200gBB/hari selama 14 hari dan 28 hari tidak berpotensi menyebabkan perubahan pada struktur histologi ren tikus putih. The prevalence of prediabetes increases every year and it is predicted that more than 470 million people will experience prediabetes in 2030. Continuous exogenous insulin induction causes insulin resistance which leads to an increase in blood glucose levels which can have an effect on renal histology. This study aims to determine the histology of the ren, which includes renal weight, Bowman's capsule thickness, proximal tubular cell size, distal tubular cell size, and ren index after being induced with exogenous insulin. This research was carried out for 6 months at the Laboratory of Structural and Functional Biology of Animals and in the cages of test animals, Department of Biology, Faculty of Science and Mathematics, Diponegoro University. This study used 21 white rats which were divided into 3 treatment groups and 7 replications. P0 (control), P1 (induced by insulin glargine 1.80 IU/200gBW/day for 14 days), and P2 (induced by insulin glargine 1.80 IU/200gBW/day for 28 days). Insulin glargine is injected subcutaneously using a xiulin pen. Renal histology preparations were made by paraffin method and Hematoxylin-Eosin staining. Observation of preparations using a photomicrograph. The research data were then analyzed by the Anova test. The results of the Anova test showed that the differences between treatments were not significantly different. The conclusion of this study was that insulin induction of 1.80 IU/200gBW/day for 14 days and 28 days did not have the potential to cause changes in the histological structure of the white rat's kidney.

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Profil Histopatologi Otot Jantung pada Tikus Hipertensi (Doca-Garam) yang Dipapar Kombinasi Ekstrak Metanolik Benalu Teh Dan Benalu Mangga (EMBTBM)

Cited by 3 publications

References 2 publications

Effects of Pamelo Orange Peel (Citrus maxima) Ethanol Extract on Macroscopic Kidney and Heart of Hypertensive Rats

Effects of Pamelo Orange Peel (Citrus maxima) Ethanol Extract on Macroscopic Kidney and Heart of Hypertensive Rats

Pengaruh Ekstrak Etanol Daun Asam Jawa (Tamarindus indica L.) terhadap Jantung Tikus Putih (Rattus norvegicus L.) yang Diinduksi Minyak Jelantah

Gambaran Histologi Ren Tikus Putih (Rattus norvegicus L.) yang Diinduksi Insulin.

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