Profil Fitokimia Ekstrak Etanol Bunga Kasumba Turate (Carthamus tinctorius L.) yang Berpotensi Sebagai Antimalaria

Hamsidi, Rini; Widyawaruyanti, Aty; Hafid, Achmad Fuad; Ekasari, Wiwied; Malaka, Muhammad Hajrul; Kasmawati, Henny; Akib, Nur Illiyyin; Wahyuni, Wiwik; Sabarudin, S

doi:10.33772/pharmauho.v4i2.6267

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…Kasumba turate mengandung senyawa fenolik flavonoid dan karotenoid yang memiliki aktivitas antioksidan, antibakteri, antivirus, antiradang, antialergi dan antikanker (Rukmana, 2014). Tanaman ini juga mengandung flavonoid, alkaloid, saponin, terpenoid, tannin dan antrakuinon (Hamsidi et al, 2018), serta quinokalkon, glikosida, hidroksi safflower yellow A,N-(P Kumaroil) dan serotonin (Zhang et al, 2016) yang memiliki aktivitas antioksidan.…”

Section: Pendahuluanunclassified

“…Rendemen ekstrak yang dihasilkan pada penelitian ini lebih besar dari penelitian sebelumnya. Hamsidi et al (2018) telah mengekstaksi 3 kg bunga kasumba turate dengan 10 L pelarut etanol 80% secara maserasi dan memperoleh hasil rendemen sebesar 5,96%. Imran (2014) juga telah mengekstraksi 0,8 kg bunga kasumba turatae dengan 15,75 L pelarut etanol 70% memperoleh rendemen sebesar 14,71%.…”

Section: Hasil Ekstraksi Bunga Kasumba Turateunclassified

“…Hasil uji fitokimia menunjukan bahwa bunga kasumba turate mengandung senyawa alkaloid, fenolik, saponin, tanin, terpenoid dan tidak mengandung steroid. Hasil penelitian ini memperkuat penelitian sebelumnya oleh Hamsidi et al (2018) Hasil uji fitokimia (Tabel 2) menunjukan bahwa bunga kasumba turate mengandung senyawa alkaloid dalam fraksi n-heksana dan etanol untuk reagen Mayer dan dalam fraksi n-heksana dan fraksi etil asetat untuk reagen Dragendorff. Menurut Marliana (2005), reaksi pembentukan warna uji alkaloid dengan pereaksi Mayer (Gambar 2) nitrogen pada alkaloid akan bereaksi dengan ion logam K+ dari kalium tetraiodomerkurat (II) membentuk kompleks kalium-alkaloid yang membentuk endapan putih sedangkan reaksi pembentukan warna pada uji alkaloid dengan dengan pereaksi Dragendorff (Gambar 3) hasil uji alkaloid positif ditandai endapan berwarna merah kuning tua (oranye).…”

Section: Hasil Uji Skrining Fitokimiaunclassified

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Efektivitas Antioksidan dari Ekstrak Bunga Kasumba Turate (Carthamus tinctorius L.) dan Potensinya Sebagai Antihiperkolesterolemia

Yasir

Momuat

Pontoh

2021

JIS

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Telah dilakukan penelitian untuk menganalisis kandungan metabolit sekunder dan efektivitas antioksidan dari ektrak etanol bunga kasumba turate (Carthamus tinctorius L.), serta potensinya sebagai antihiperkolesterolemia. Bunga kasumba turate yang telah dikeringanginkan, dihaluskan dan dimaserasi dengan pelarut etanol 70%, lalu dievaporasi pelarutnya. Ekstrak etanol (EE) yang diperoleh dipartisi berturut-turut dengan pelarut n-heksana, etil asetat dan air, sehingga diperoleh ekstrak fraksi n-heksana (FH), etil asetat (FEA) dan air (FA). Selanjutnya, EE, FH, FEA, dan FA diuji kandungan metabolit sekundernya (metode Harborne) dan efektivitas antioksidannya (metode DPPH). Hasil penelitian menunjukkan bahwa EE dan FEA mengandung alkaloid, fenolik, flavonoid, saponin, tanin dan triterpenoid. FA mengandung fenolik, flavonoid, saponin, tanin dan triterpenoid. FH mengandung alkaloid, flavonoid dan triterpenoid. Efektivitas antioksidan bunga kasumba turate (dinyatakan dalam IC50) yang tertinggi pada FEA, diikuti FH, FA dan EE, dengan nilai IC50 53,59, 75,45, 77,43, dan 89.,9 µg/mL. Hasil kajian menunjukkan bahwa bunga kasumba turate dapat menghambat oksidasi LDL dan menurunkan kadar kolesterol darah. Penelitian ini menyimpulkan bahwa ekstrak bunga kasumba turate memiliki efektivitas antioksidan yang kuat dan efek antihiperkolesterolemia.Kata kunci: Antihiperkolesterolemia; antioksidan; Carthamus tinctorius L.; etanolAntioxidant Effectiveness of Kasumba Turate Flower Extract (Carthamus tinctorius L.) and Its potential as an AntihypercholesterolemiaABSTRACTResearch has been carried out to analyze the secondary metabolite content and antioxidant effectiveness of the ethanol extract of kasumba turate flower (Carthamus tinctorius L.), as well as its potential as antihypercholesterolemia. Kasumba turate flowers that have been dried and mashed, macerated with 70% ethanol solvent, then evaporated the solvent. The ethanol extract (EE) obtained was partitioned successively with n-hexane, ethyl acetate and water as solvents, so that the extracts of the n-hexane (FH), ethyl acetate (FEA) and water (FA) fractions were obtained. Furthermore, EE, FH, FEA, and FA were tested for their phytochemical content (Harbourne method) and antioxidant effectiveness (DPPH method). The results showed that EE and FEA contained alkaloids, phenolics, flavonoids, saponins, tannins and triterpenoids. FA contains phenolics, flavonoids, saponins, tannins and triterpenoids. FH contains alkaloids, flavonoids and triterpenoids. The antioxidant effectiveness of casumba turate flower (expressed in IC50) was highest in FEA, followed by FH, FA and EE, with IC50 values of 53,59, 75,45, 77,43, and 89,19 g/mL, respectively. The results of the literature review show that kasumba turate flowers can inhibit LDL oxidation and reduce blood cholesterol levels. This study concluded that kasumba turate flower extract has a strong antioxidant effectiveness and antihypercholesterolemic effect.Keywords: Antihypercholesterolemia; antioxidant; Carthamus tinctorius L.; ethanol

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Section: Pendahuluanunclassified

Section: Hasil Ekstraksi Bunga Kasumba Turateunclassified

Section: Hasil Uji Skrining Fitokimiaunclassified

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Efektivitas Antioksidan dari Ekstrak Bunga Kasumba Turate (Carthamus tinctorius L.) dan Potensinya Sebagai Antihiperkolesterolemia

Yasir

Momuat

Pontoh

2021

JIS

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Steroid Compounds Isolation from Carthamus tinctorius Linn as Antimalarial

Hamsidi

Wahyuni

Fristiohady

et al. 2021

RJPT

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Carthamus tinctorius Linn, also known as safflower, is a plant with the potential of being used in the production of antimalarial drugs. The purpose of this study was to isolate and identify the steroid compounds in the safflower and determine its antimalarial activity in vitro. The isolation process was conducted through extraction and chromatography methods. Then, the characterization of the isolated compounds was conducted through spectroscopic techniques which include Fourier Transform Infrared Spectroscopy (FT-IR), NMR 1-D (1H and 13C-NMR), and NMR 2-D (HMQC, HMBC, and H-H COZY) as well as comparing data with the existing literatures. In addition, the tests conducted were with variations of isolate concentrations (10, 1, 0.1, 0.01, and 0.001 μg/mL) against 3D7 strain of Plasmodium falciparum. Based on the FT-IR spectroscopic data, the steroid compounds isolated from safflowers might be stigmasterols. In addition, the isolates had -OH functional group in the region of 3431 cm-1, C-O in the region of 1053 cm-1, and Csp3-H in regions of 2960, 2934, and 2865 cm-1. The NMR 1-D data showed presence of 29 carbon atoms, while the protons were 48 in number. Furthermore, the IC50 value of the compound was 34.03 μg/mL with a percentage inhibition of 43.92% against the growth of P. falciparum. Therefore, it was classified as inactive agent in inhibiting the growth of malaria parasites, however, it could be used as a marker compound in C. tinctorius Linn extract.

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Characteristic of the Ethanol Extract of Carthamus tinctorius L. Flowers and its Antioxidant Activity

Hamsidi

Adianti

Septriana

et al. 2023

RJPT

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Safflower plant (Carthamus tinctorius L.) has been reported to be effective for treating people with measles, hypertension, stomach ache, malaria and cholesterol issues. This study aims to assess specific and non-specific parameters of characteristics for the ethanol extract of C. tinctorius L. flowers and its antioxidant properties. The specific parameters include the extract’s organoleptic properties, while the non-specific parameters include the drying shrinkage, microbial contamination content, ash content, as well as the extract’s solubility in water and ethanol according to Materia Medica Indonesia and Pharmacopeia Herbal Indonesia. An antioxidant activity assay was performed using the DPPH method. According to the results, the deep red, viscous organoleptic extract had a distinctive odor, as well as an astringent or chelate taste, and an Rf value of 0.79. Furthermore, the extract contained 0.12% of water-soluble compounds, 0.22% ethanol-soluble compounds, and several phytochemical groups, including flavonoids, saponins, triterpenoids, tannins, quinones, and steroids. According to the non-specific parameters, the extracts demonstrated 1.02%, 0.2237%, 0.96%, 0.23%, and 0.91% for weight, acid-insoluble ash content, dry shrinkage, ash content, and water content, respectively. Meanwhile, the microbial contaminant load was ˂ 1.0 × 10-1 colony/mL, alongside ˂ 1.0 × 10-1 colony/mL of mold/yeast. The C. tinctorius L. ethanol extract was observed to show significant antioxidant activities, evidenced by an IC50 which measured 2.511ppm, based on DPPH analysis. A similar outcome was also reported for the ethanol flower extract, which was further determined to fulfil both non-specific and specific standardization parameters.

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Profil Fitokimia Ekstrak Etanol Bunga Kasumba Turate (Carthamus tinctorius L.) yang Berpotensi Sebagai Antimalaria

Cited by 3 publications

References 6 publications

Efektivitas Antioksidan dari Ekstrak Bunga Kasumba Turate (Carthamus tinctorius L.) dan Potensinya Sebagai Antihiperkolesterolemia

Efektivitas Antioksidan dari Ekstrak Bunga Kasumba Turate (Carthamus tinctorius L.) dan Potensinya Sebagai Antihiperkolesterolemia

Steroid Compounds Isolation from Carthamus tinctorius Linn as Antimalarial

Characteristic of the Ethanol Extract of Carthamus tinctorius L. Flowers and its Antioxidant Activity

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