Production, storability, and regeneration of shoot tips of potato (Solanum tuberosum L.) encapsulated in calcium alginate hollow beads

Nyende, Aggrey Bernard; Schittenhelm, Siegfried; Mix-Wagner, Gunda; Greef, Jöörg-Micheal

doi:10.1079/ivp2003442

Cited by 57 publications

(31 citation statements)

References 16 publications

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“…Potential advantages of synthetic seeds include their designation as ''genetically identical materials'', ease of handling and transportation, along with increased efficiency of in vitro propagation in terms of space, time, labor and over-all cost (Nyende et al 2003). Although synthetic seeds have been widely utilized for micro propagation and conservation of various medicinal plant species (Ara et al 2000;Mandal et al 2000;Anand and Bansal 2002;Nyende et al 2003;Manjkhola et al2005;Singh et al2006a, b;Narula et al 2007;Faisal and Anis 2007;Ray and Bhattacharyaa 2008;Lata et al 2009), the genetic stability of synthetic seed-derived plantlets remains relatively unknown with the exception of a recent report on Ananus comosus (L.) Merr (Gangopadhyay et al 2005).…”

Section: Introductionmentioning

confidence: 99%

An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

Srivastava

Khan

Banerjee

2009

Plant Cell Tiss Organ Cult

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Regrowth of encapsulated microshoots, using alginate encapsulation, of Cineraria maritima reached 82.35% following 6 months of storage. Amongst developing plantlets, 33.33% exhibited formation of multiple shoots at the onset of regrowth and 11.76% demonstrated simultaneous formation of shoots and roots. Healthy root formation was observed in plantlets following 2 weeks of their transfer to half-strength Murashige and Skoog medium containing 1.0 mg l -1 a-naphthalene acetic acid. Plants were transplanted to the greenhouse in three batches with 90% frequency of survival. Molecular analysis of randomly selected plants from each batch was conducted using 20 random amplified polymorphic DNA (RAPD) markers. Of 20 primers tested, 14 produced amplification products, and a total of 69 bands with an average of 4.93 bands per primer were observed. Of these 69 scorable bands, only 20% of bands were polymorphic. Cluster analysis of the RAPD profiles revealed an average similarity coefficient of 0.944 thus confirming molecular stability of plants derived from encapsulated microshoots following 6 months of storage.

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Section: Introductionmentioning

confidence: 99%

An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

Srivastava

Khan

Banerjee

2009

Plant Cell Tiss Organ Cult

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“…Potential advantages of synthetic seeds include their designation as "genetically identical materials", ease of handling and transportation, along with increased efficiency of in vitro propagation in terms of space, time, labour and overall cost (Nyende et al 2003 ). Synthetic seeds have been widely utilized for micropropaga-tion and conservation of various med icin al c limb ers (Kumar and Thomas 2012 ;Narula et al 2007 ;Muthiah et al 2013 ;Sharma and Shahzad 2012 ;Dhir and Shekhawat 2013 ;Baskaran et al 2014 ) and there are reports where genetic stability of synthetic seed-derived plantlets was also carried out (Dhir and Shekhawat 2013 ).…”

Section: Advantages Of Synthetic Seed Technologymentioning

confidence: 99%

Advancement in Encapsulation Techniques for Conservation of Climbers

Shaheen

Shahzad

2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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Encapsulation technology has recently revolutionized the production and conservation programme of elite and threatened germplasm throughout the globe. This technology has made the exchange programme possible between different laboratories at an ease. Synthetic seed production not only scaled up extensive and commercial production of plants but also has economized the requirements of space and time. Synseed technology has been utilized for commercial and industrial production of different agricultural, ornamental, medicinal and woody climbers. The present chapter deals with studies on synthetic seed production and conservation of some important woody climbers, viz. Tylophora indica , Ceropegia bulbosa , Dioscorea bulbifera , Clitoria ternatea , Mondia whitei , Decalepis hamiltonii and Caesalpinia bonduc .

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“…The technique of synseed has been widely studied and works with various plant species including fruits, cereals, medicinal plants, vegetables, ornamentals, forest trees, and orchids (Ballester et al 1997;Gonzalez-Benito et al 1997;Standardi and Piccioni 1998;Ara et al 2000;Nyende et al 2003;Chand and Singh 2004;Tsvetkov and Hausman 2005;Malabadi and Staden 2005;Naik and Chand 2006;Faisal et al 2006;Faisal and Anis 2007;Antonietta et al 2007;Micheli et al 2007;Pintos et al 2008;Rai et al 2008;Sundararaj et al 2010;Singh et al 2010;Germana et al 2011;Mishra et al 2011). In addition, during cold storage, encapsulated nodal segments require no transfer to fresh medium, thus reducing the cost of maintaining germplasm cultures (West et al 2006).…”

Section: Advancement In Plant Tissuementioning

confidence: 99%

Review of Literature

Varshney¹,

Anis²

2014

Trees: Propagation and Conservation

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Gottlieb Haberlandt (Math Naturwiss 111:69-92), a German plant physiologist, for the first time initiated the work on tissue culture. His work arose as a research tool and attempts were made to culture the isolated, fully differentiated cells in nutrient medium in vitro as early as 1898. The theoretical basis of tissue culture lies in the cell theory given by Schleiden and Schwann (1838-1839). Practically, this technique stands on the concept of "totipotency," i.e., each cell has the ability to regenerate into a new plant. The field finds a wide range of applications starting from mass clonal propagation to plant improvement, molecular biology, bio-processing as well as a basic research tool. It has advanced the production in forestry and agriculture to many folds. There are a number of reviews published on tissue culture of woody and tree species which provide the wide-ranging micropropagation reports of various plant species. In this way, this chapter highlights the current review in tree tissue culture in vitro and micropropagation of other valuable plants, their significance, and the wide scope existing for investigations on mass multiplication and conservation of these plants.

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Production, storability, and regeneration of shoot tips of potato (Solanum tuberosum L.) encapsulated in calcium alginate hollow beads

Cited by 57 publications

References 16 publications

An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage

Advancement in Encapsulation Techniques for Conservation of Climbers

Review of Literature

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