Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources

Abstract

Poly(hydroxybutyric acid) (PHB) and other biodegradable polyesters are promising candidates for the development of environment-friendly, totally biodegradable plastics. The use of cane molasses and corn steep liquor, two of the cheapest substrates available in Egypt, may help to reduce the cost of producing such biopolyesters. In this work, the effect of different carbon sources was studied. Maximum production of PHB was obtained with cane molasses and glucose as sole carbon sources (40.8, 39.9 per mg cell dry matter, respectively). The best growth was obtained with 3% molasses, while maximum yield of PHB (46.2% per mg cell dry matter) was obtained with 2% molasses. Corn steep liquor was the best nitrogen source for PHB synthesis (32.7 mg per cell dry matter), on the other hand, best growth was observed when ammonium chloride, ammonium sulphate, ammonium oxalate or ammonium phosphate were used as nitrogen sources.

Production of biopolyester poly(3-hydroxybutyrate) by Bacillus cereus RCL 02, a leaf endophyte of Ricinus communis L.

“…7). Sugarcane molasses, the byproducts of sugar industry served as the cheap non-conventional source of carbon along with vitamins and other minor constituents for effective and sustainable production of P(3HB) by the isolate RCL 02 similar to those reported for Alcaligenes eutrophus [33], Bacillus megaterium [19] and Bacillus sp. JMa5 [15].…”

“…Development of newer fermentation strategies along with use of nonconventional renewable carbon sources including different plant oils [17,18], molasses [19], oil cake extracts [20], crude glycerol [21] and agricultural wastes [22] have been explored to reduce the cost of PHAs production and making the process economically viable.…”

Effect of carbon and nitrogen sources on simultaneous production of α-amylase and green food packaging polymer by Bacillus sp. CFR 67

“…Sodium nitrate, an inorganic nitrogen source, significantly (P<0.05) supported for the production of higher amount of PHA, but the yield of α-amylase was not significant (Table 1). Ammonium acetate supported production of both amylase and PHA and it is known to enhance PHA synthesis by B. megaterium (Gouda et al 2001). Among the organic nitrogen sources tested, beef extract significantly (P<0.05) supported higher synthesis of PHA, however, amylase production was not significant.…”

Molasses : dampak negatif pada ruminansia

“…Molasses digunakan secara luas sebagai sumber karbon untuk denitrifikasi, fermentasi anaerobik (Pazouki et al, 2000), pengolahan limbah aerobik (Gouda et al, 2001), dan diaplikasikan pada pelestarian lingkungan perairan (Thakare et al, 2013;Tansengco et al, 2016). Selain pemanfaatan tersebut diatas, molasses juga digunakan dalam industri makanan manusia (Bakhiet and Al-Mokhtar, 2015) maupun industri peternakan dari jumlah yang kecil untuk mengurangi partikel debu dalam pakan sampai jumlah yang besar sebagai sumber mineral dan energi bentuk cair (Zaid et al, 2013).…”