Production of biopolyester poly(3-hydroxybutyrate) by Bacillus cereus RCL 02, a leaf endophyte of Ricinus communis L.

Das, Rituparna; Pal, Arundhati; Paul, A. K.

doi:10.24896/jmbr.2017744

Cited by 9 publications

(12 citation statements)

References 13 publications

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“…These reports focussed primarily on the process optimization for PHA production using sugarcane molasses with very little emphasis on the material properties of the accumulated biopolyesters. in mineral salts medium containing glucose as the sole source of carbon [13]. The present study demonstrates the bioprocessing as well as partial characterization of P(3HB-co-3HV) copolyesters produced by the endophytic isolate RCL 02 following bioconversion of sugarcane molasses as the sole source of carbon in the growth medium.…”

Section: Introductionmentioning

confidence: 62%

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Bioconversion of sugarcane molasses to poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by endophytic Bacillus cereus RCL 02

2019

jabb

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Sugarcane molasses, the by-product of sugar industry are rich in nutrients, growth factors and minerals and are efficiently utilized by the microorganisms for growth and production of primary as well as secondary metabolites of commercial importance. In this communication, we report the utilization of sugarcane molasses as the sole source of carbon for the production of copolymers of polyhydroxyalkanoates (PHAs) with improved material properties. The endophytic bacterium Bacillus cereus RCL 02 (MCC 3436) produced 7.8 g/L of PHA when grown in MS medium with 4% (w/v) sugarcane molasses under batch cultivation. Proton nuclear magnetic resonance ( 1 H NMR) analysis revealed that the copolymer so produced contain 12.4 mol% 3-hydroxyvalerate (3HV) along with 87.6 mol% 3-hydroxybutyrate (3HB). The copolyester, P(3HB-co-12.4 mol%-3HV) has been isolated and purified following standard solvent extraction method and partially characterized by Fourier-transform infrared (FTIR), X-ray diffraction (XRD), thermogravimetric (TG) and differential scanning calorimetric (DSC) analysis. The material and thermal properties of the copolyester so produced indicated its potential for industrial application.

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Section: Introductionmentioning

confidence: 62%

“…glucose, sucrose, maltose etc. [13]. It may also be presumed that molasses served not only as a potential source of carbon but also provided vitamins and other essential growth factors for effective growth and sustainable production of the biopolyester by the isolate RCL 02.…”

Section: Growth Kinetics and Accumulation Of Phamentioning

confidence: 99%

Bioconversion of sugarcane molasses to poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by endophytic Bacillus cereus RCL 02

2019

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“…This study revealed a 2.81-fold enhancement (2.87 to 8.07g/L) of P(3HB) production by the endophytic B. cereus RCL 02 after optimization using response surface methodology in comparison to the preliminary OVAT studies. 11 Production of biopolyester (8.07g/L) by the leaf endogenous isolate B. cereus appeared to be quite satisfactory and compared well with other Bacillus spp. so far reported.…”

Section: Validation and Confirmation Of The Modelmentioning

confidence: 84%

“…Selection of the fermentation variables and their lower and higher levels were decided based upon the results obtained from 'OVAT' experiments. 11 Growth (Y 1 ) and P(3HB) production (Y 2 ) were executed in a set of 12 experiments following the PB design. The significance level (p value) of each variable was determined using Fisher's F-test.…”

Section: Experimental Design and Optimization Using Wrsmmentioning

confidence: 99%

Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacillus cereus RCL 02

Das¹,

Pal²,

Dutta³

et al. 2018

JABB

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Abbreviations: P(3HB), poly (3-hydroxybutyrate); WRSM, weighted response surface methodology; PB, plackett-burman; CCD, central composite design; NMR, nuclear magnetic resonance; PHAs, polyhydroxyalkanoates; OVAT, one variable at a time; RSM, response surface methodology; DoE, design of experiment AbstractApplication of statistical models in developing strategies for process optimization and enhanced product yield is well established. The present study is focused to enhance poly (3-hydroxybutyrate) [P(3HB)] production by Bacillus cereus RCL 02, a leaf endophyte of oleaginous plant Ricinus communis L. using weighted response surface methodology (WRSM). The most influencing process variables for growth and bio-polyester accumulation were initially selected through Plackett-Burman (PB) designed experiments. Further, WRSM was applied to navigate the experimental data obtained in accordance with the central composite design (CCD). Interaction of three independent variables viz. carbon source (glucose), nitrogen source (yeast extract) and initial pH of the medium exerted most significant impact on biomass formation as well as P(3HB) production. A second order polynomial equation obtained from multiple regression analysis and the interaction studies from surface plots helped to determine the optimum concentrations of glucose (25g/L), yeast extract (4g/L) and initial pH (7.6). Validation of this statistical model was executed using point prediction tool of WRSM where-from the optimum values of all three significant variables were employed and experiments were conducted. Under such optimized conditions the endophytic isolate produced 8.07g/L of P(3HB), which was very close to the predicted value (7.80g/L) and thereby validated the model. Finally, the identity of the intracellularly accumulated bio-polyester of B. cereus RCL 02 was confirmed by proton nuclear magnetic resonance (1H NMR) spectroscopic analysis. Citation: Rituparna D, Arundhati P, Dutta G, et al. Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacillus cereus RCL 02. Citation: Rituparna D, Arundhati P, Dutta G, et al. Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacillus cereus RCL 02. Citation: Rituparna D, Arundhati P, Dutta G, et al. Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacillus cereus RCL 02. Citation: Rituparna D, Arundhati P, Dutta G, et al. Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacillus cereus RCL 02. Citation: Rituparna D, Arundhati P, Dutta G, et al. Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacillus cereus RCL 02. Citation: Rituparna D, Arundhati P, Dutta G, et al. Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacill...

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“…However, B. megaterium showed maximum PHB accumulation when glucose was used as carbon source (Hori et al, 2002). Bacillus species has been reported to accumulate PHB up to 90% of cell dry weight by utilizing soy molasses oligosaccharides like raffinose without the need for nutrient limitation (Full et al, 2006;Das et al, 2017).…”

Section: Production Of Phbmentioning

confidence: 99%

Screening of PHB biopolymer producing Bacillus cereus from municipal sludge waste

Murali¹,

Mamatha²,

Mathiyazhagan³

2019

Int. J. Curr. Res. Biosci. Plant Biol.

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Biopolymers are the rarely produced bio products which are used as an alternative for common plastic and some microbes can even digest them. In this study we have confirmed that Bacillus cereus can produce such bio polymers. B. cereus, isolated from sludge soil sample was standalone which showed substantial PHB production (4.2 ±0.25 g/l) after 48 h of fermentation as examined by spectrophotometric analysis. Time-course (24-96 h) analysis for PHB production indicated that PHB was a growth-associated product and its accumulation significantly increased during the exponential phase and reached maximum after 48 h (5.6 ±0.25 g/l). The maximum PHB yield was achieved after 48-72 h of fermentation (6.5±0.18 g/l). The FT-IR analysis, illustrates bands at 3280.92 and 2924.09 cm −1 corresponding to aliphatic ester carbonyl C=O of R COA and C-H stretch, respectively. Other absorption bands at 1633.71 and 1533.09 cm −1 , represent the presence of O-H bonding and C-H bond of aliphatic compounds, respectively. The band at 1633.71 cm −1 considered as diagnostic signal for PHB. The band found at 1,380.17 cm −1 is assigned to symmetric wagging of CH3 groups. The 1 H-NMR spectra showed the presence of three signals, it's the characteristic of the polymer of PHB.

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Production of biopolyester poly(3-hydroxybutyrate) by Bacillus cereus RCL 02, a leaf endophyte of Ricinus communis L.

Cited by 9 publications

References 13 publications

Bioconversion of sugarcane molasses to poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by endophytic Bacillus cereus RCL 02

Bioconversion of sugarcane molasses to poly(3-hydroxybutyrate-co-3- hydroxyvalerate) by endophytic Bacillus cereus RCL 02

Application of weighted response surface methodology for enhanced poly (3-hydroxybutyrate) production by endophytic bacillus cereus RCL 02

Screening of PHB biopolymer producing Bacillus cereus from municipal sludge waste

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