Production of multifunctional lipases by Penicillium verrucosum and Penicillium brevicompactum under solid state fermentation of babassu cake and castor meal

Silva, Marceli Fernandes; Freire, Denise Maria Guimarães; Castro, Aline Machado de; Luccio, Marco Di; Mazutti, Márcio A.; Oliveira, J. Vladimir; Treichel, Helen; Oliveira, Débora de

doi:10.1007/s00449-010-0455-1

Cited by 21 publications

(8 citation statements)

References 36 publications

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“…The highest lipase production was obtained with 1% of peptone enrichment (C/N ratio of 11.7, 28 Ug ) [74]. Molasses, urea and soybean oil concentrations as well as moisture content were tested for lipase production by Penicillium brevicompactum in SSF using babassu cake and castor meal as solid support [75]. For babassu cake, the highest lipase activity was obtained for a moisture content of 70% and 2% of soybean oil.…”

Section: Regulation Of Lipase Production Concerning the Medium Composmentioning

confidence: 99%

Fungal Lipase Production by Solid-State Fermentation

Ramos-Sánchez¹,

Cujilema-Quitio²,

Julián-Ricardo³

et al. 2015

J Bioprocess Biotech

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Lipases are one of the most promising enzymes in the chemical and biopharmaceutical industries. Numerous applications have been reported including fine chemistry, detergents formulation and biodiesel synthesis. Lipases are commonly produced by a wide variety of yeasts and filamentous fungi in submerged fermentation or solid-state fermentation. Filamentous fungi and yeasts usually behave more efficiently in solid-state fermentation and show greater productivities when compared to submerged fermentation. Although filamentous fungi adequately growth in solid-state fermentation, there are some limitations for cultivating them by this process. Here, a review is made about lipase production in solid-state fermentation. This includes the analysis of solid-state fermentation as a promising technology, characterization of growth media and ambient factors such as moisture, pH and temperature.

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Section: Regulation Of Lipase Production Concerning the Medium Composmentioning

confidence: 99%

Fungal Lipase Production by Solid-State Fermentation

Ramos-Sánchez¹,

Cujilema-Quitio²,

Julián-Ricardo³

et al. 2015

J Bioprocess Biotech

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“…Although there are some difficulties when working at elevated temperatures, such as concerning shorter life of sensors and seals, secondary reactions or lower biomass yields, there are also important advantages like the higher reaction rates, reduced viscosity of the medium, less diffusion restrictions and higher solubility of most of the substrates and products [3,6].…”

Section: Introductionmentioning

confidence: 99%

“…The market of lipolytic enzymes has a turnover of over a billion US dollars per year, and it comprises a wide variety of applications in the areas of detergents, paper, oleochemistry and petrochemistry, and in the production of food ingredients and enantiopure pharmaceuticals [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Scaling-up the production of thermostable lipolytic enzymes from Thermus aquaticus YT1

López

Domínguez

Deive

et al. 2012

Bioprocess Biosyst Eng

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The lipolytic enzymes synthesized by Thermusaquaticus YT1 present extremely interesting properties of thermostability (more than 70% of activity after 12 days at 80°C and a half-life time of 1 h at 95°C), which point out the interest of proposing efficient strategies to successfully tackle the scale-up of the production process. In this study,viable scaling-up of the production process was implemented,and relevant aspects affecting the enzyme synthesis, such as the mineral composition of the culture medium, the aeration and the agitation have been evaluated.A strategy combining the modification of the culture medium and the aeration degree was also approached by adding perfluorocarbons, compounds which improve the availability of oxygen in the culture medium. An opposite response of biomass and lipolytic activity to the aeration conditions was found between scales (about 600 U L(-1) at high aeration levels in flask vs. 150 U L(-1) at high aeration rates in reactor), which further demonstrates the important role of the hydrodynamic conditions on the suitable development of the biological process. In all cases, the cultures were kinetically characterized and the Luedeking and Piret model turned out to be a valuable tool to conclude that the produced lipolytic enzyme is a growth-associated metabolite, no matter the medium and the scale.

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“…Most lipases produced by fungi are from Rhizopus sp., Aspergillus sp., Penicillium sp., and Rhizomucor sp. (Cihangir and Sarikaya, 2004;Gutarra et al, 2009;Mahapatra et al, 2011;Damaso et al, 2008;Silva et al, 2011;Kempka et al, 2008). In general, the optimum pH for bacterial lipase is neutral or alkaline, and it is more thermostable than plant lipase.…”

Section: Introductionmentioning

confidence: 99%

“…Agro-industrial wastes including wheat bran (Mahadik et al, 2002;Mahapatra et al, 2011), sugarcane bagasse (Rodriguez et al, 2006), and babassu cake (Silva et al, 2011) have been used as alternative media for the production of lipase using solid-state fermentation (SSF). Jatropha curcas seed cake is the by-product of oil extraction, containing a high concentration of protein and carbohydrates.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium

et al. 2017

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Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potential as a medium for lipase production. The objective of this research was to characterize lipase from Aspergillus niger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate specificity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate specificity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and v max about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipase. Based on these results, we conclude that Jatropha seed cake is a suitable medium for lipase production.

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Production of multifunctional lipases by Penicillium verrucosum and Penicillium brevicompactum under solid state fermentation of babassu cake and castor meal

Cited by 21 publications

References 36 publications

Fungal Lipase Production by Solid-State Fermentation

Fungal Lipase Production by Solid-State Fermentation

Scaling-up the production of thermostable lipolytic enzymes from Thermus aquaticus YT1

Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium

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