Production of Cellulase from Oil Palm Biomass as Substrate by Solid State Bioconversion

Alam, Md. Zahangir; Muhammad, Nurdina; Mahmat, Mohd Erman

doi:10.3844/ajassp.2005.569.572

Cited by 39 publications

(13 citation statements)

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“…1). A total amount of 4 kg media (wet basis), based on the compositions of the laboratory-scale experiment, was placed in the bioreactor [6]. The sample was inoculated with 10% (v/w) using the same spore concentration as in the EF experiment.…”

Section: Solid State Bioconversion (Ssb)mentioning

confidence: 99%

“…A moisture content of 60% was maintained during the solid state fermentation using 30% distilled water, 20% mineral solution and 10% inoculum [6]. The process conditions of solid state bioconversion were adapted from the optimization study conducted by Alam et al [6].…”

Section: Solid State Bioconversion (Ssb)mentioning

confidence: 99%

“…Tel. : +60 3 61964571 fax: +60 3 61964442. bioconversion of EFB into value added products, such as compost, citric acid, enzymes [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Solid state bioconversion of oil palm empty fruit bunches for cellulase enzyme production using a rotary drum bioreactor

Alam

Mamun

Qudsieh

et al. 2009

Biochemical Engineering Journal

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Section: Solid State Bioconversion (Ssb)mentioning

confidence: 99%

Section: Solid State Bioconversion (Ssb)mentioning

confidence: 99%

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Solid state bioconversion of oil palm empty fruit bunches for cellulase enzyme production using a rotary drum bioreactor

Alam

Mamun

Qudsieh

et al. 2009

Biochemical Engineering Journal

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“…The main purpose of screening is to select fungi with desired characteristics intended for various applications, e.g. bioremediation of industrial wastewater and contaminated sites1–3 and lignocellulolytic enzyme production 4–8. In general, most of the screenings are based on the colourimetric method where colour changes of synthetic chemicals in the media are associated with specific fungal ligninolytic enzyme activities.…”

Section: Introductionmentioning

confidence: 99%

A quantitative method for fungal ligninolytic enzyme screening studies

Ang

Ngoh

Chua

2011

Asia-Pacific J Chem Eng

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Screening is conducted to select a fungus with desired characteristics intended for various applications, e.g. bioremediation and enzyme production. A qualitative method was used in this study for screening of fungal ligninolytic enzyme activities. The fungal ligninolytic activity was correlated with its growth from the screenings to identify a suitable fungus for solid substrate fermentation. Four strains of fungi, namely, Phanerochaete chrysosporium, Pycnoporus sanguineus, Phlebia radiata and Pleurotus sajor-caju, were screened for their ligninolytic enzyme activities using guaiacol and remazol brilliant blue-R (RBB-R) as screening reagents. The screenings were conducted at both, the optimal growth temperature of each fungus and 35 • C. All the fungi, except P. radiata, showed positive guaiacol oxidation and RBB-R decolourisation activities. On the basis of the results, P. sajor-caju may be a suitable fungus for use in bioconversion of lignocellulose because it maintained guaiacol oxidation and RBB-R decolourisation activities at 35 • C. Its halo-to-colony area ratios of guaiacol oxidation and RBB-R decolourisation after 6 days were 7.0 and 2.7, respectively. In the subsequent growth study, the measured colony area was closely correlated with the dry fungal cell weight with a regression value (R 2 ) of 0.971. Thus, the colony size could be used as an indicator of fungal growth if the screening media do not significantly slow the growth of fungus. 

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“…Kadar air merupakan faktor penting dalam fermentasi padat. Kadar air berpengaruh pada pertumbuhan mikroorganisme, biosintesis, dan sekresi enzim (Alam et al 2005). Faktor lainnya adalah pH yang merupakan salah satu faktor dalam pertumbuhan mikroorganisme.…”

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Produksi Enzim Selulase olehAspergillus niger pada Ampas Sagu

Idiawati¹,

Harfinda²,

Arianie³

2015

J.Nat.Indones

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Production of cellulase by Aspergillus niger was carried out by growing the cultureson sago waste. Sago waste contains cellulose that has not been used optimally. Cellulose is a polysaccharide consisting of glucose monomers linked by β-1,4glycosides bonds. Glycoside bonds in cellulose can be enzymatically hydrolyzed into glucose with cellulase enzymes. Solid fermentation used to produce cellulase on sago waste as substrate was influenced by pH (3 to 6), moisture content (40% to 85%), and fermentation time (4 to 10 days). Products of the cellulase enzyme activity was measured by phenolsulfuric acid method. The results showed that the highest cellulase enzyme activity was 0.172 U/mL obtained at 85% moisture content, pH 5, and 8 days of fermentation time. ABSTRAKProduksi enzim selulase oleh Aspergillus niger pada ampas sagu telah dilakukan. Ampas sagu adalah salah satu bahan yang mengandung selulosa di mana ampas sagu belum dimanfaatkan secara optimal. Selulosa merupakan polisakarida yang terdiri atas monomer glukosa yang dihubungkan oleh ikatan β-1,4-glikosida. Ikatan glikosida pada selulosa dapat dihidrolisis menjadi glukosa secara enzimatik dengan enzim selulase. Fermentasi padat digunakan untuk menghasilkan enzim selulase pada substrat ampas sagu yang dipengaruhi oleh faktor pH (3 sampai 6), kadar air (40% sampai 85%) dan waktu fermentasi (4 sampai 10 hari). Produk aktivitas enzim selulase diukur dengan metode fenol-asam sulfat. Hasil penelitian menunjukkan bahwa aktivitas enzim selulase tertinggi adalah 0,172 U/mL yang diperoleh pada kadar air 85%, pH 5, dan waktu fermentasi 8 hari.

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Production of Cellulase from Oil Palm Biomass as Substrate by Solid State Bioconversion

Cited by 39 publications

References 0 publications

Solid state bioconversion of oil palm empty fruit bunches for cellulase enzyme production using a rotary drum bioreactor

Solid state bioconversion of oil palm empty fruit bunches for cellulase enzyme production using a rotary drum bioreactor

A quantitative method for fungal ligninolytic enzyme screening studies

Produksi Enzim Selulase olehAspergillus niger pada Ampas Sagu

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