“…The maximum production of reducing sugars was (1.521 mg g -1 ) with maximum activity (0.23 U g -1 ) respectively. The result obtained was in agreement with Alam et al (2009) who observed that the highest cellulase activity was achieved when carried out SSF was incubated at 32°C using Trichoderma harzianum T 2008 grown on empty fruit bunches.…”
Section: Effect Of Temperature On Cellulase Productionsupporting
TWENTY isolates of cellulase producing fungi were isolated from six samples of rotted rice straw which collected from different localities in El-Gharbia Governorate (Basyion), Kafrelsheikh Governorate (Sakha) and El-Dakahlia Governorate (El-Mansoura). On the basis of hydrolysis zone surrounding the colonies, an efficient fungus isolate (F7) for highest cellulolytic activity were chosen. According to morphological and biochemical characteristics as well as 18S rRNA sequence indicated that the isolate belonged to Aspergillus tubingensis KY615746. Effect of culture conditions for production of cellulase enzyme by A. tubingensis were investigated under Submerged Fermentation (SMF) and Solid State Fermentation (SSF) techniques. The optimum conditions for cellulase production by A. tubingensis were the best inoculum size was found to be 3%, pH 4, temperature 30 °C and incubation period 6th day where the reducing sugars attained (0.675, 0.728, 0.731 and 0.913 mg ml -1 ) with maximum activity (0.050, 0.055, 0.053 and 0.063 U ml -1 ), respectively for SMF conditions.On the other hand, the maximum cellulase activity under SSF technique with moisture level 10g : 30ml were (0.16 Ug -), incubation period 9 th day, inoculum size 4 ml:10g, pH 5 and temperature 30 °C where the reducing sugars were (1.412, 1.532, 1.551 and 1.521 mg g -1 ) with maximum activity (0.20, 0.22, 0.23 and 0.23 Ug -1 ), respectively. Based on the results, it may be concluded that rice straw waste can be a potential substrate for produce cellulase by SSF technique.
“…The maximum production of reducing sugars was (1.521 mg g -1 ) with maximum activity (0.23 U g -1 ) respectively. The result obtained was in agreement with Alam et al (2009) who observed that the highest cellulase activity was achieved when carried out SSF was incubated at 32°C using Trichoderma harzianum T 2008 grown on empty fruit bunches.…”
Section: Effect Of Temperature On Cellulase Productionsupporting
TWENTY isolates of cellulase producing fungi were isolated from six samples of rotted rice straw which collected from different localities in El-Gharbia Governorate (Basyion), Kafrelsheikh Governorate (Sakha) and El-Dakahlia Governorate (El-Mansoura). On the basis of hydrolysis zone surrounding the colonies, an efficient fungus isolate (F7) for highest cellulolytic activity were chosen. According to morphological and biochemical characteristics as well as 18S rRNA sequence indicated that the isolate belonged to Aspergillus tubingensis KY615746. Effect of culture conditions for production of cellulase enzyme by A. tubingensis were investigated under Submerged Fermentation (SMF) and Solid State Fermentation (SSF) techniques. The optimum conditions for cellulase production by A. tubingensis were the best inoculum size was found to be 3%, pH 4, temperature 30 °C and incubation period 6th day where the reducing sugars attained (0.675, 0.728, 0.731 and 0.913 mg ml -1 ) with maximum activity (0.050, 0.055, 0.053 and 0.063 U ml -1 ), respectively for SMF conditions.On the other hand, the maximum cellulase activity under SSF technique with moisture level 10g : 30ml were (0.16 Ug -), incubation period 9 th day, inoculum size 4 ml:10g, pH 5 and temperature 30 °C where the reducing sugars were (1.412, 1.532, 1.551 and 1.521 mg g -1 ) with maximum activity (0.20, 0.22, 0.23 and 0.23 Ug -1 ), respectively. Based on the results, it may be concluded that rice straw waste can be a potential substrate for produce cellulase by SSF technique.
“…Results demonstrated that MSW can be used as an inexpensive lignocellulosic material for the production of cellulase enzymes. Research has revealed that T. reesei can produce 13.4 FPU/g cellulase activity using water hyacinth [8]; 154.58 FPU/g using sugar cane bagasse [10]; 8.2 FPU/g using oil palm empty fruit bunches [11]; and 1.16 FPU/g using rice bran [12] as substrates. Similarly it has been reported that A. Niger could produce 24 FPU/g cellulase activity using wheat straw as a substrate [13].…”
This paper explores the possibility of using an industrially processed municipal solid waste (MSW) for cellulase enzyme production via solid state fermentation (SSF) by Trichoderma reesei and Aspergillus niger. Both fungi grew well on the MSW substrate and production of cellulase enzymes was optimized for temperature, moisture content, inoculation and period of incubation. The effect of additional minerals, and alternative carbon and nitrogen sources were also examined.Following optimization a cellulase activity of 26.10 ± 3.09 FPU/g could be produced using T. reesei at 30°C with a moisture content of 60% with an inoculums of 0.5 million spores/g and incubation for 168 hours. Addition of extra nitrogen and/or carbon did not improve cellulase accumulation. Acid or alkali pretreatment of MSW led to reduced cellulase production. Crude enzymes produced from MSW by T. reesei were evaluated for their ability to release glucose from MSW. A cellulose hydrolysis yield of 24.7% was achieved, which was close to that obtained using a commercial enzyme. Results demonstrated that MSW can be used as an inexpensive lignocellulosic material for the production of cellulase enzymes. Research has revealed that T. reesei can produce 13.4 FPU/g cellulase activity using water hyacinth [8]; 154.58 FPU/g using sugar cane bagasse [10]; 8.2 FPU/g using oil palm empty fruit bunches [11]; and 1.16 FPU/g using rice bran [12] as substrates. Similarly it has been reported that A. Niger could produce 24 FPU/g cellulase activity using wheat straw as a substrate [13]. Other substrates such as banana peel, rice straw, corn cob residue, rice husk, banana fruit stalk, and coconut coir pith have all being used for cellulase production [14,15].
Optimizing Cellulase Production fromAlthough MSW can contain a high lignocellulosic content, there has been limited research into the use of MSW for cellulase production. Other research demonstrated the possibility of using MSW to produce cellulase via SSF. But by using raw not autoclave MSW [16,17]. In this paper, we report an example of using an industrially processed MSW for cellulase production and then the use of the resulting "cellulase cocktail" for the enzymatic hydrolysis of MSW.
Materials and Methods
Municipal solid wasteMunicipal solid waste (MSW) was kindly supplied by Wilson Steam Storage Ltd (UK). The MSW had been subjected to commercial steam 15 min, respectively. After autoclaving, samples were neutralized to pH 7.0 using either 1 M NaOH or 1 M H 2 SO 4 as appropriate. The samples were then centrifuged at 5000 rpm (4472 g) for 10 min. The solid fraction (pellet) was rinsed three times by adding distilled water to the pellet and re-centrifuged. The solid fraction was then dried overnight at room temperature.
Journal of Fundamentals of Renewable Energy and ApplicationsMicroorganisms: Two filamentous fungi, Trichoderma reesei QM6a and Aspergillus niger N402 were kindly donated by Professor David Archer (University of Nottingham, UK). Procedures for storing and cultivating T. reesei and A. n...
“…They are also used in homogenization of powders, and in different drying processes (Porter et al, 1973). Besides traditional drum applications, novel applications can be found as bioreactors or organic waste composters (Kalamdhad and Kazmi, 2008;Alam et al, 2009). …”
Mixing performance in continuous rotary drums has been studied. The video analysis method was developed to evaluate different configurations of straight lifters in the rotary drum. The method converts a captured video into a single image, called stack image. The color marker tracking was estimated based on the color saturation of the stack image. Coefficients of variation and mixing indices were calculated from the color saturation profiles for different straight blade lifter configurations. The video analysis method was confronted to the impulse response of acid concentrations in water solutions. The developed analysis method has been superior with viscous fluids compared to traditional tracer impulse method in mixing evaluations. Water and 1% CMC-water solution were used in this mixing study for covering broadly different viscous materials. The drum lengthwise results for one lifter configuration were obtained from a single experiment due to the block representation of the image analysis method. It enables mixing analysis of axial segments and interaction analysis of mixer configurations. Thus, the axial mixing can be studied in more detail with rotary drums. The increase of lifters, residence time, and tip speed improved axial mixing in the studied experimental setup.
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