Process optimization for antifungal chitinase production by Trichoderma harzianum

Nampoothiri, K. Madhavan; Baiju, Thekke V.; Sandhya, Chandran; Sabu, Abdulhameed; Szakács, George; Pandey, Ashok

doi:10.1016/s0032-9592(03)00282-6

Cited by 130 publications

(74 citation statements)

References 22 publications

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“…This statement is also supported by another research that stated chitinolytic activity of T. harzianum reached its optimum amount in between day 2 and day 3 [40]. No significant change of glucosamine production after it has reached its optimum point is also in accordance with a research [41] which stated after 172 hours, glucosamine fermentation using T. harzianum mold starter did not undergo significant increase.…”

Section: Glucosamine Productionsupporting

confidence: 86%

Study of Glucosamine Production from Shrimp Shells by Fermentation Using Trichoderma harzianum

Hardoko

Sasmito

Puspitasari

et al. 2017

JELS

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Shrimp shells are one of chitin sources in Indonesia which is potential to be converted into multifunctional glucosamine. This research was aimed to study the glucosamine production by fermentation using Trichoderma harzianum. Method used was experimental fermentation with pH treatment of 3-5 and fermentation duration of 10-20 days, and designed using Response Surface Methodology (RSM). Results showed that fermentation duration of 10-20 days has reached the optimum point of glucosamine production from shrimp shells using T. harzianum. The highest production of Dglucosamine in fermentation using T. harzianum occurred on initial pH of 5.41 and fermentation duration of 15 days (18,294.95 ppm), while the highest N-Acethyl-D-Glucosamine production occurred on initial pH of 3.00 and fermentation duration of 20 days (127,000.00 ppm).

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Section: Glucosamine Productionsupporting

confidence: 86%

Study of Glucosamine Production from Shrimp Shells by Fermentation Using Trichoderma harzianum

Hardoko

Sasmito

Puspitasari

et al. 2017

JELS

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“…Its entomopathogenicity is close related to its ability of synthesize hydrolytic enzymes. Despite several studies on Trichoderma chitinase production has been published [5,[9][10][11][12], the most studied strains are T. harzianum and T. viride, and to date, in the published studies, no reports on the production of chitosanase by Trichoderma spp. were found.…”

Section: Introductionmentioning

confidence: 99%

Effect of pH and Temperature on Enzyme Activity of Chitosanase Produced Under Solid Stated Fermentation by Trichoderma spp.

et al. 2011

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Trichoderma strains were extensively studied as biocontrol agents due to their ability of producing hydrolytic enzymes, which are considered key enzymes because they attack the insect exoskeleton allowing the fungi infection. The present work aimed to evaluate the ability of chitosanase production by four Trichoderma strains (T. harzianum, T. koningii, T. viride and T. polysporum) under solid stated fermentation and to evaluate the effect of pH and temperature on enzyme activity. pH strongly affected the enzyme activity from all tested strains. Chitosanase from T. harzianum and T. viride presented optimum activity at pH 5.0 and chitosanase from T. koningii and T. polysporum presented optimum activity at pH 5.5. Temperature in the range of 40-50°C did not affect enzyme activity. T. polysporum was found as the most promising strain to produce chitosanase with maximal enzyme activity of about 1.4 IU/gds, followed by T. viride (*1.2 IU/gds) and T. harzianum (1.06 IU/gds).

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“…One variable was varied at a time keeping other variable constant, it included the incubation time (24-120 h), incubation temperature (20-45°C), pH (4)(5)(6)(7)(8)(9)(10)(11)(12), salt concentration (2-6% NaCl), effect of nitrogen sources such as peptone, yeast extract, urea, ammonium chloride, ammonium sulphate, casein and various carbon sources such as arabinose, fructose, D-glucose, mannose, starch, xylose. For each parameter optimization, three sets of independent experiments were carried out and the average values were reported.…”

Section: Estimation Of Exo-chitinase Activitymentioning

confidence: 99%

“…In this context, solid state fermentation (SSF) has emerged as a promising technology for the enzyme production from cheaply available agro-industrial residues such as wheat bran, rice bran, oil cakes, etc. [5]. Optimization of growth parameters and media components for chitinase production in SSF following one-factor-at-a-time has been widely reported [6].…”

Section: Introductionmentioning

confidence: 99%

Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

et al. 2011

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The growth conditions for chitinase production by Trichoderma asperellum UTP-16 in solid state fermentation was optimized using response surface methodology based on central composite design. The chitinase production was optimized, using one-factor at a time approach, with six independent variables (temperature, pH, NaCl, incubation period, nitrogen and carbon sources) and 3.31 Units per gram dry substrate (U gds -1 ) exo-chitinase yield was obtained. A 21.15% increase was recorded in chitinase activity (4.01 U gds -1 ) through surface response methodology, indicates that it is a powerful and rapid tool for optimization of physical and nutritional variables. Further, efficiency of crude enzyme was evaluated against phytopathogenic Fusarium spp. and a mycelial growth inhibition up to 3.5-6.5 mm was achieved in well diffusion assay. These results could be supplemented as basic information for the development of enzyme based formulation of T. asperellum UTP-16 and its use as a biocontrol agent.

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Process optimization for antifungal chitinase production by Trichoderma harzianum

Cited by 130 publications

References 22 publications

Study of Glucosamine Production from Shrimp Shells by Fermentation Using Trichoderma harzianum

Study of Glucosamine Production from Shrimp Shells by Fermentation Using Trichoderma harzianum

Effect of pH and Temperature on Enzyme Activity of Chitosanase Produced Under Solid Stated Fermentation by Trichoderma spp.

Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

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