Ikan asin merupakan produk tradisional yang tingkat konsumsi dan popularitasnya menurun, sehingga perlu dikembangkan menjadi produk pangan fungsional dengan memanfaatkan sumber serat yang kurang termanfaatkan. Penelitian ini bertujuan untuk menentukan jenis dan konsentrasi bahan pengikat pada pembuatan nugget ikan asin berserat pangan dengan memanfaatkan ampas tahu. Penelitian menggunakan rancangan acak lengkap faktorial (RALF) dengan faktor pertama adalah perbedaan jenis tepung sebagai binder (tepung maizena, tepung tapiokadan tepung terigu) dan faktor kedua adalahkonsentrasi tepung (10%, 20%, 30%) terhadap berat daging ikan dan ampas tahu. Hasil penelitian menunjukkan bahwa jenis tepung tidak berpengaruh terhadap karakteristik fisik dan organoleptik nugget, tetapi interaksi antara jenis dan konsentrasi tepung berpengaruh pada rendemen, tekstur, kadar air dan tingkat kesukaan nugget ikan asin. Tepung maizena, tepung tapioka maupun tepung terigu dapat digunakan sebagai bahan pengikat nugget. Nugget ikan asin terpilih adalah nugget dengan bahan pengikat tepung tapioka konsentrasi 30%. dengan nilai tekstur 14,30 Newton (secara organoleptik agak empuk), kadar air 27,48%, kadar protein 6,88%, kadar lemak 5,84%, kadar abu 2,31%, serat pangan 9,30%, karakteristik organoleptik agak berrasa asin dengan aroma ikan asin dan secara keseluruhan disukaii panelis.
Background and Aim: The study about the antiallergenic properties of inedible fish body parts is still limited. Therefore, this study aimed to characterize the charcoal from the body parts of Kerandang fish (Channa pleurophthalmus Blkr) and identify its antiallergenic properties.
Materials and Methods: This study used some non-edible body parts extracted from the Kerandang fish (i.e., the scalp, scales, and dorsal, pectoral, ventral, anal, and caudal fins) using a maceration method with different solvents (ethanol, ethyl acetate, and chloroform). The identification of active compounds in the extract was carried out using liquid chromatography– high-resolution mass spectrometry (LC-HRMS) analysis, while the antihyaluronidase activity was determined using the antihyaluronidase test. The highest charcoal antihyaluronidase activity-extract was applied to ovalbumin-induced mice for 7 days with various doses (10, 15, and 20 mg/kg). The specific immunoglobulin E (IgE) was measured using enzyme-linked immunosorbent assay on day 8.
Results: Our LC-HRMS analysis showed that the active compound of charcoal in the caudal fins of Kerandang fish was hexadecanamide. The highest inhibition (IC50) of hyaluronidase was found in the ethyl acetate extract of fish caudal fins at a concentration of 4 mg/mL. We found that 15 mg/kg body weight of charcoal of fish caudal fins suppressed IgE expression in male mice.
Conclusion: Our findings indicate that the charcoal of non-edible body parts of Kerandang and one of its constituent, hexadecanamide, may have strong antiallergic effects.
Objectives: The aim of this research was to determine the effect of heating temperature of mangrove Rhizophora mucronata fruit extract toward its inhibition activity of α-glucosidase.
Methods:Research method used was an experimental method with the treatment of heating temperature (70°C, 80°C, 90°C, and 100°C) of crude tannin extract of mangrove R. mucronata fruit on inhibition activity toward α-glucosidase.Results: IC 50 of unheated extract and extracts that were heated at 70°C, 80°C, 90°C, and 100°C are adalah 3.38 ppm, 2.79 ppm, 2.97 ppm, 3.19 ppm, and 3.52 ppm, respectively. Heating until 100°C only decreased its inhibition activity of α-glucosidase of about 3.87% compared to unheated extract. Mangrove R. mucronata fruit extract contains phytochemical compounds, i.e., alkaloids, saponin, flavonoids, tannin, and terpenoids. IC 50 of this extract is lower compared to IC 50 of acarbose (antidiabetic medicine), which as IC 50 value of 13.27 ppm.
Conclusion:Mangrove R. mucronata fruit extract is quite heat resistant and is potential to be an antidiabetic functional food.
Teh hijau telah lama dipercaya memiliki kandungan antioksidan alami yang tinggi. Penelitian ini untuk memperoleh cara penyeduhan yang tepat dan identifikasi senyawa bioaktif sebagai antioksidan dalam teh hijau daun Sonneratia alba. Percobaan dirancang menggunakan Rancangan Acak Lengkap (RAL) faktorial dengan faktor suhu dan lama penyeduhan. Penyeduhan terbaik menggunakan suhu 100 o C selama 10 menit yang menghasilkan total tanin tertinggi sebesar 3,18% dan nilai IC 50 terhadap DPPH sebesar 96,5 ppm. Aktivitas antioksidan (FRAP) sebesar 105 ppm, kadar katekin 0,46% dan total fenol 84,94 mgGAE/g. Teh hijau daun Sonneratia alba positif terhadap alkaloid, flavonoid, saponin, tanin. Senyawa bioaktif yang diduga sebagai antioksidan sebagian besar dari famili fenolik.
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