Probiotics enhance recovery from malnutrition and lessen colonic mucosal atrophy after short-term fasting in rats

Dock, Diana B; Latorraca, Márcia Queiroz; Aguilar-Nascimento, José Eduardo de; Gomes-da-Silva, Maria Helena Gaíva

doi:10.1016/j.nut.2004.01.013

Cited by 26 publications

(23 citation statements)

References 28 publications

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“…On the other hand, the supplementation with probiotics (G4) provided a protective effect to the mucosa; this damage is generally observed in protein malnourishment (Viteri and Schneider 1974, Rodrigues et al 1985, Torrejais et al 1995, Natali et al 2000, Schoffen et al 2005, De Azevedo et al 2007, Hermes et al 2008. It is important to point out that the eutrophic animals were also supplemented with probiotics (G2) and presented thicknening of the mucosa when compared to G1, corroborating the studies that describe the proliferative effect induced by these microorganisms (Dock et al 2004a, AguilarNascimento et al 2006, Ng et al 2009). In a parallel study of the jejunum of the same animals, it was observed that an equivalent result to these parameters (J.F.…”

Section: Resultssupporting

confidence: 75%

“…Several studies have demonstrated that probiotics estimulate cell proliferation of the immune system associated to the intestinal mucosa (Erickson and Hubbard 2000, Cano and Perdigón 2003, Villena et al 2006, De Souza et al 2007, Dewan et al 2007, kaburagi et al 2007, Pitsouni et al 2009), which may contribute to the control of infections, as well as to their prevention in the case of diseases derived from nutritional deficiency. On the other hand, there are few studies that evaluated the action of probiotics on the intestinal morphology of malnourished animals, and were mostly restricted only to the study of the mucosa (Allori et al 2000, Cano et al 2002, Dock et al 2004a, b, Dock-Nascimento et al 2007. A single study examined the different strata of the intestinal wall by examining only the small intestine (J.F.…”

Section: Introductionmentioning

confidence: 99%

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Probiotics prevent growth deficit of colon wall strata of malnourished rats post-lactation

Lima

Azevedo

Hermes-Uliana

et al. 2012

An. Acad. Bras. Ciênc.

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The objective of this study was to analyze morphometrically the colon wall strata of malnourished rats supplemented with probiotics. Sixteen recently weaned Wistar rats (Rattus norvegicus) were distributed into four groups: animals that received commercial chow (G1, n = 4); animals that received the same feed as G1 and were supplemented with probiotics (G2, n = 4); animals that received chow with 4% of proteins (G3, n = 4); animals that received the same feed as G3 and were supplemented with probiotics (G4, n = 4). After 12 weeks, the proximal colon was collected and submitted to histological processing. Three-µm cuts were stained with H.E., Periodic Acid Schifff (P.A.S.) + diasthasis solution and Alcian Blue (A.B.) pH 2.5 and pH 1.0. The morphometric analysis of the intestinal wall showed that the supplementation with ABT-4 probiotic culture prevents the growth deficit of colon wall strata that normally occurs in malnourished rats right after lactation. Besides, no alteration was observed in the proportion of the number of globet cells in relation to the number of enterocytes in malnourished rats, regardless of the supplementation with probiotics.

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Section: Resultssupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Probiotics prevent growth deficit of colon wall strata of malnourished rats post-lactation

Lima

Azevedo

Hermes-Uliana

et al. 2012

An. Acad. Bras. Ciênc.

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“…This suggests an extra-mucosal trophic effect on the colonic wall that deserve further studies. Other previous study in a model of malnutrition has shown benefits of probiotics in enhancing mucosal trophism 17 . Probiotics may increase the production of SCFA which are directly trophic to the colonic mucosa 18 .…”

Section: Discussionmentioning

confidence: 86%

Perioperative administration of probiotics: effects on immune response, anastomotic resistance and colonic mucosal trophism

et al. 2006

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Purpose:To investigate the effects of perioperative administration of diet containing probiotics on the immune response, anastomotic resistance, and colonic mucosal trophism in rats underwent left-sided colonic anastomosis. Methods: Sixty adults Wistar rats were randomized to two groups (A and B) with 30 animals each to receive or not a diet supplemented with probiotics (Streptococcus thermophilus e Lactobacillus helveticus) during pre-and postoperative period. They underwent laparotomy followed by left colon section and immediate anastomosis. Groups of 15 animals were killed either on the 4 th (groups A1 and B1) or 7 th PO (groups A2 and B2) days. The outcome variables were serum proteins, albumin, globulins and IgA, lenght and weight of the colon, mucosal weight, DNA content and anastomotic bursting pressure. Results: Colonic weight was greater in probiotic group compared to controls (1,69±0,34g vs 1,48±0,26g; p<0.05). Total serum proteins and globulin were higher in probiotic animals (proteins = 7,05±0,97g/dl vs 6,57±0,66g/dl e globulins = 5,1±0,62g/dl vs 4,68±1,1g/dl; p<0.05). Serum IgA increased from basal to post-operative days only in the probiotic group (p<0.01). Conclusions: Perioperative probiotics enhanced the immune response and colonic weight. Probiotics failed to increase anastomotic resistance. Key-words: Probiotics. Wound Healing. Immunoglobulin A. Immunity, mucosal. Anastomosis, Surgical. Colon. Intestinal Mucosa. Surgery. RESUMOObjetivo: Investigar os efeitos da administração peroperatória de dieta acrescida de probióticos sobre a resposta imunológica, resistência anástomótica e trofismo da mucosa colônica em ratos submetidos a anastomose do cólon esquerdo. Métodos: 60 ratos Wistar adultos foram randomizados em dois grupos (A e B), cada um com 30 animais, de acordo com o uso ou não de suplementação pré e pós-operatória de probióticos (Streptococcus thermophilus e Lactobacillus helveticus). Foram submetidos a laparotomia com secção e anastomose imediata do cólon esquerdo, e sacrificados em grupos de 15 animais no 4º (grupos A1 e B1) ou 7º dia de pós-operatório (grupos A2 e B2). As variáveis estudadas foram concentração plasmática de proteínas totais, albumina, globulinas e IgA; peso e comprimento do cólon sem fezes, peso da mucosa, conteúdo de DNA da mucosa e pressão anastomótica de ruptura. Resultados: Observou-se aumento do peso do intestino grosso sem fezes no grupo A (probiótico) quando comparado ao controle (1,69±0,34g vs 1,48±0,26g; p<0.05). Houve aumento de proteínas totais e globulinas no grupo probiótico (proteínas = 7,05±0,97g/dl vs 6,57±0,66g/dl e globulinas = 5,1±0,62g/dl vs 4,68±1,1g/dl; p<0.05). A IgA aumentou entre os níveis basais e o pós-operatório apenas no grupo probiótico (p<0.01). As demais variáveis não apresentaram diferenças entre diversos grupos estudados. Conclusão: O uso peri-operatório de probióticos melhorou a resposta imunológica e o peso do cólon. Não houve diferença na resistência anastomotica. Descritores: Probióticos. Cicatrização de feridas. Imunoglobu...

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“…Nos últimos anos, os probióticos vêm sendo utilizados na prevenção de anormalidades gastrointestinais e na desnutrição induzida experimentalmente em animais 40,41 . Por outro lado, evidências recentes de experimentos com animais mostram que a microbiota intestinal pode interferir no processo de regulação do metabolismo energético 42 .…”

Section: Tabela 4 -Lactobacilos E Bifidobactérias (Ufc/g De Fezes) Deunclassified

Lactobacilli and bifidobacteria in the feces of schoolchildren of two different socioeconomic groups: children from a favela and children from a private school

et al. 2009

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Objective: To determine the number of lactobacillus and bifidobacterium colonies in the feces of schoolchildren from two different socioeconomic levels. Methods:We analyzed fecal samples of children aged 6 to 10 years without gastrointestinal symptoms or recent use of antimicrobials. The first group included 86 children living in a favela in the city of Osasco, state of São Paulo, southeastern Brazil. The second group included 36 children attending a private school in the same city. Body mass index (BMI) was used to assess nutritional status according to the reference values of the National Center for Health Statistics (NCHS). Specific anaerobic culture media were used for isolation of colonies for 48 and 72 hours at 37 °C. The number of colonies was determined using the plate-counting method. Results:The mean lactobacillus (1.125 x 10 9 colony-forming units, CFU/g) and bifidobacterium (1.675 x 10 9 CFU/g) counts in the private school group were higher (p < 0.001) than those in the favela group: 0.250 x 10 9 and 0.350 x 10 9 CFU/g, respectively. In the favela group, children with BMI z score < -1.0 standard deviation (SD) (n = 28) showed lower mean (p < 0.05) lactobacillus (0.100 x 10 9 CFU/g) and bifidobacterium (0.095 x 10 9 CFU/g) counts than the children with BMI ≥ -1.0 SD (n = 57): 0.350 x 10 9 and 0.420 x 10 9 CFU/g, respectively. Conclusion:The microbiota of schoolchildren living in unfavorable environmental conditions shows lower numbers of fecal lactobacillus and bifidobacterium colonies, especially in children with lower BMI values.J Pediatr (Rio J). 2009;85(4):307-314: Lactobacillus, bifidobacterium, socioeconomic factors, nutritional status, environmental exposure, enteropathies. ResumoObjetivo: Determinar o número de colônias de lactobacilos e bifidobactérias nas fezes de crianças escolares, pertencentes a dois estratos socioeconômicos.Métodos: Foram analisadas amostras de fezes de crianças com idade entre 6 e 10 anos sem sintomas gastrointestinais ou uso recente de antimicrobianos. O primeiro grupo foi constituído por 86 crianças, moradoras em uma favela localizada no município de Osasco (SP). O segundo grupo foi constituído por 36 crianças matriculadas em uma escola particular da mesma cidade. O estado nutricional foi avaliado usando o índice de massa corporal (IMC) de acordo com os valores de referência do National Center for Health Statistics (NCHS). O isolamento das colônias foi realizado em meios de cultura específicos em anaerobiose, durante 48 e 72 horas a 37 °C. A determinação do número foi feita pelo método da contagem em placa.Resultados: A mediana de lactobacilos (1,125 x 10 9 unidades formadoras de colônia, UFC/g) e bifidobactérias (1,675 x 10 9 UFC/g) na escola particular foi superior (p < 0,001) ao do grupo da favela: 0,250 x 10 9 e 0,350 x 10 9 UFC/g, respectivamente. No grupo da favela, crianças com escore z de IMC < -1,0 desvio padrão (n = 28) apresentaram menor mediana (p < 0,05) de lactobacilos (0,100 x 10 9 UFC/g) e bifidobactérias (0,095 x 10 9 UFC/g) em relação às cria...

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Probiotics enhance recovery from malnutrition and lessen colonic mucosal atrophy after short-term fasting in rats

Cited by 26 publications

References 28 publications

Probiotics prevent growth deficit of colon wall strata of malnourished rats post-lactation

Probiotics prevent growth deficit of colon wall strata of malnourished rats post-lactation

Perioperative administration of probiotics: effects on immune response, anastomotic resistance and colonic mucosal trophism

Lactobacilli and bifidobacteria in the feces of schoolchildren of two different socioeconomic groups: children from a favela and children from a private school

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