Probiotic characterization of Lactiplantibacillus plantarum HOM3204 and its restoration effect on antibiotic-induced dysbiosis in mice

Zhang, S; Wang, T; Zhang, D; Wang, X; Zhang, Z; Lim, C; Lee, S

doi:10.1111/lam.13683

Cited by 7 publications

(7 citation statements)

References 57 publications

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“…Probiotics can inhibit intestinal pathogens by producing antibacterial compounds, compete for rejection by consuming limited nutritional resources or adhering to epithelial cells, or stimulate intrinsic microbial activity ( 2 ). This is consistent with the research results of Zhang ( 54 ) that Lactiplantibacillus plantarum HOM3204 can significantly increase the relative abundance of Lactobacillus in AAD mice and decrease the relative abundance of Enterococcus . At the same time, we also found that the efficacy of strain 2-33 on AAD mice was time- and dose-dependent, which was similar to the results of Ling’s ( 55 ) study on the efficacy of Clostridium butyricum and Bifidobacterium infanti on AAD mice.…”

Section: Discussionsupporting

confidence: 93%

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Bao

et al. 2022

Front. Nutr.

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Diarrhea is one of the common adverse reactions in antibiotic treatment, which is usually caused by the imbalance of intestinal flora, and probiotics play an important role in the structure of intestinal flora. Therefore, this experiment studied the regulatory effect of Lactiplantibacillus plantarum 2-33 on antibiotic-associated diarrhea (AAD) mice. First, the AAD mice model was established by the mixed antibiotic solution of gentamicin sulfate and cefradine. Then, the physiological indexes and diarrhea of mice were observed and recorded by gastric perfusion of low dose (1.0 × 107 CFU/ml), medium dose (1.0 × 108CFU/ml), and high dose (1.0 × 109 CFU/ml) strain 2-33. 16S rRNA gene V3-V4 regions were sequenced in colon contents of mice in control group, model group, self-healing group, and experimental group, respectively, and the diversity of intestinal flora and gene function prediction were analyzed. The results showed that the intestinal flora of AAD mice was not significantly regulated by gastric perfusion of strain 2-33 to 7 days, but the relative abundance and diversity of intestinal flora of AAD mice were significantly improved by gastric perfusion to 14 days (p < 0.05). In addition, at the genus level, the relative abundance of Lactobacillus increased significantly, and the relative abundance of Enterococcus and Bacillus decreased significantly (p < 0.05). In addition, the regulation of strain 2-33 on intestinal flora of AAD mice was time- and dose-dependent, short-term gastric perfusion, and low dose had no significant effect (p > 0.05). Strain 2-33 can significantly increase the levels of anti-inflammatory cytokines IL-4 and IL-10, significantly decrease the levels of proinflammatory cytokines TNF-α and IFN-γ (p < 0.05), and can also adjust carbohydrate metabolism, amino acid metabolism, and energy metabolism to normal levels, thus accelerating the recovery of intestinal flora structure of AAD mice. In summary, strain 2-33 can improve the structure and diversity of intestinal flora of AAD mice, balance the level of substance and energy metabolism, and play a positive role in relieving diarrhea, maintaining and improving the intestinal microecological balance.

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Section: Discussionsupporting

confidence: 93%

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Bao

et al. 2022

Front. Nutr.

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“…In a previous study, we isolated a Lpb. plantarum strain, HOM3204, from homemade pickled cabbage [ 9 ]. In vitro, Lpb.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro, Lpb. plantarum HOM3204 showed strong tolerance to simulated gastric and intestinal juice, high adhesion to Caco-2 cells, and good antimicrobial activity [ 9 ]. It significantly recovered the intestinal flora in ampicillin-induced dysbiotic mice by decreasing the abundance of Enterococci , while increasing the abundance of Lactobacilli and Bifidobacterium .…”

Section: Introductionmentioning

confidence: 99%

“…It significantly recovered the intestinal flora in ampicillin-induced dysbiotic mice by decreasing the abundance of Enterococci , while increasing the abundance of Lactobacilli and Bifidobacterium . The strain also enhanced the antioxidant capacity by increasing the levels of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Whole Genome Sequence of Lactiplantibacillus plantarum HOM3204 and Its Antioxidant Effect on D-Galactose-Induced Aging in Mice

Zhang¹,

Shin

Wang³

et al. 2023

J. Microbiol. Biotechnol.

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Lactiplantibacillus plantarum , previously named Lactobacillus plantarum , is a facultative, homofermentative lactic acid bacterium widely distributed in nature. Several Lpb. plantarum strains have been demonstrated to possess good probiotic properties, and Lpb. plantarum HOM3204 is a potential probiotic strain isolated from homemade pickled cabbage plants. In this study, whole-genome sequencing was performed to acquire genetic information and predict the function of HOM3204, which has a circular chromosome of 3,232,697 bp and two plasmids of 48,573 and 17,060 bp, respectively. Moreover, various oxidative stress-related genes were identified in the strain, and its antioxidant activity was evaluated in vitro and in vivo. Compared to reference strains, the intracellular cell-free extracts of Lpb. plantarum HOM3204 at a dose of 10 10 colony-forming units (CFU)/ml in vitro exhibited stronger antioxidant properties, such as total antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate, superoxide dismutase activity, and glutathione (GSH) content. Daily administration of 10 9 CFU Lpb. plantarum HOM3204 for 45 days significantly improved the antioxidant function by increasing the glutathione peroxidase activity in the whole blood and GSH concentration in the livers of D-galactose-induced aging mice. These results suggest that Lpb. plantarum HOM3204 can potentially be used as a food ingredient with good antioxidant properties.

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“…The results of this study showed that the consumption of GABA enhanced the modulation of norepinephrine and triggered the overexpression of the endothelin-1 protein, which is one of the most relevant factors affecting the hypertension modulation. These wide benefits of GABA and probiotic microorganisms, like L. plantarum strains, have opened a new possibility to address the demand of new functional ingredients (Zhang et al, 2022a;Jin et al, 2022). Considering the abovementioned background, the objective of the present study was the characterisation of the probiotic ability and safety of L. plantarum K16 strain isolated from Kimchi.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the probiotic potential of Lactiplantibacillus plantarum K16 and its ability to produce the postbiotic metabolite γ-aminobutyric acid

Diez-Gutiérrez

Vicente

Sáenz

et al. 2022

Journal of Functional Foods

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Probiotic characterization of Lactiplantibacillus plantarum HOM3204 and its restoration effect on antibiotic-induced dysbiosis in mice

Cited by 7 publications

References 57 publications

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Whole Genome Sequence of Lactiplantibacillus plantarum HOM3204 and Its Antioxidant Effect on D-Galactose-Induced Aging in Mice

Characterisation of the probiotic potential of Lactiplantibacillus plantarum K16 and its ability to produce the postbiotic metabolite γ-aminobutyric acid

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