Whole Genome Sequence of Lactiplantibacillus plantarum HOM3204 and Its Antioxidant Effect on D-Galactose-Induced Aging in Mice

Abstract

Lactiplantibacillus plantarum , previously named Lactobacillus plantarum , is a facultative, homofermentative lactic acid bacterium widely distributed in nature. Several Lpb. plantarum strains have been demonstrated to possess good probiotic properties, and Lpb. plantarum HOM3204 is a potential probiotic strain isolated from homemade pickled cabbage plants. In this study, whole-genome sequencing was performed to acquire genetic information and predict the function of HOM3204, which has a circular chromosome of 3,232,697 bp and two plasmids of 48,573 and 17,060 bp, respectively. Moreover, various oxidative stress-related genes were identified in the strain, and its antioxidant activity was evaluated in vitro and in vivo. Compared to reference strains, the intracellular cell-free extracts of Lpb. plantarum HOM3204 at a dose of 10 10 colony-forming units (CFU)/ml in vitro exhibited stronger antioxidant properties, such as total antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging rate, superoxide dismutase activity, and glutathione (GSH) content. Daily administration of 10 9 CFU Lpb. plantarum HOM3204 for 45 days significantly improved the antioxidant function by increasing the glutathione peroxidase activity in the whole blood and GSH concentration in the livers of D-galactose-induced aging mice. These results suggest that Lpb. plantarum HOM3204 can potentially be used as a food ingredient with good antioxidant properties.

Lactiplantibacillus plantarum CCFM8661 alleviates d-galactose-induced brain aging in mice by the regulation of the gut microbiota

“…For example, L. plantarum has a strong antioxidant capacity and can alleviate the oxidative stress caused by D-galactose exposure. [22][23][24] Moreover, L. plantarum will extend the lifespan of mice by protecting the intestinal barrier and inhibiting apoptosis and inflammation in intestinal cells. 25 L. plantarum also alleviates aging symptoms in mice by modulating the gut microbiota and SCFAs.…”