Pro-Angiogenesis Role of LINC00662 From Esophageal Squamous Cell Carcinoma Cells-Derived Extracellular Vehicles

Abstract

Objective: LINC00662 is oncogenic in some human cancers, but no much was revealed concerning to its specific action in tumor angiogenesis. Given that, our study investigated the role of LINC00662 from esophageal squamous cell carcinoma (ESCC) cells-derived extracellular vehicles (EVs) in angiogenesis through microRNA (miR)-195-5p/vascular endothelial growth factor A (VEGFA) axis.Methods: Clinical tissue samples were collected from patients with ESCC, in which LINC00662, miR-195-5p and VEGFA expression was analyzed. ESCC cells were transfected, from which EVs were isolated. Human umbilical vein endothelial cells (HUVECs) were co-cultured with the pretreated EVs. After that, viability, colony formation ability, invasion, migration and tube formation ability of HUVECs were observed. Tumor xenograft in nude mice was performed to detect the effect of LINC00662, miR-195-5p or EV specific inhibitor GW4869 on tumor development.Results: LINC00662 and VEGFA were upregulated while miR-195-5p was downregulated in the cancer tissue of patients with ESCC. EVs derived from ESCC cells promoted viability, colony formation ability, invasion and tube formation ability of HUVECs. Downregulation of LINC00662 or upregulation of miR-195-5p reversed the promotion of EVs derived from ESCC cells on the viability, colony formation ability, invasion and tube formation ability of HUVECs in vitro and in vivo. VEGFA overexpression reversed EVs carrying restored miR-195-5p induced effects on HUVECs in vitro.Conclusion: In summary, elevated LINC00662 transferred by ESCC cells-derived EVs induces angiogenesis through downregulating miR-195-5p and upregulating VEGFA.

Is the Non-Coding RNA miR-195 a Biodynamic Marker in the Pathogenesis of Head and Neck Squamous Cell Carcinoma? A Prognostic Meta-Analysis

“…Several studies have confirmed that miR-195 is downregulated in carcinoma tissues, for example, the most recent studies of Li et al [ 33 ], found that it was downregulated in squamous cell carcinoma of the esophagus, while a recent review of the literature conducted by Xu et al [ 34 ], they found that it downregulated in very many solid tumors, indicating that its overexpression is generally associated with a tumor growth inhibition effect.…”

The Prognostic Role of miR-195 in Head and Neck Squamous Cell Carcinoma: Systematic Review with Meta-Analysis and Trial Sequential Analysis

“…Many studies con rm that miR-195 is downregulated in carcinoma tissues (29), for example citing the most recent studies, Li et al nds it downregulated in squamous cell carcinoma of the esophagus (29), while a recent review of the literature conducted by Xu et al nds it downregulated in very many solid tumors indicating that its overexpression is generally associated with a tumor growth inhibition effect (30).…”

Extracellular Vesicles as Delivery Shippers for Noncoding RNA‐Based Modulation of Angiogenesis: Insights from Ischemic Stroke and Cancer

“…[175,224,226,239,225] In contrast, the remaining nine lncRNAs (GAS5, LINC01915, LINC00662, FAM225A, SNHG16, LINC00161, SNHG11, CCAT1, and UCA1), GAS5 with LINC01915 were downregulated in respiratory and colorectal cancer, [180,213] the left were upregulated in digestive cancer tissues and promoted oesophageal adenocarcinoma, hepatocellular and pancreatic cancer progression. [187,190,196,201,236,237,248] It is well documented that EVs and miRNAs are irreplaceable intercellular communication tools and have received great attention due to their modulation of angiogenesis. Numerous studies have been proposed on miRNAs from EVs and most studies are related to cancer.…”

“…Seventy‐six publications were found in this section, from Iran, Japan, China, Korea, and India, performed between 2013 and 2022. [ 127,174–248 ] Of these ncRNAs, 3 circRNAs, 14 lncRNAs, and 45 microRNAs were differentially expressed between healthy individuals and various cancers. Concerning circRNAs (namely, circHIPK3, circFNDC3B, and circCMTM3 derived from EVs) are upregulated and shown to regulate metatherian, tissue inhibitor of metalloproteinase 3, SOX9 through miR‐124‐3p, miR‐937‐5p, miR‐3619‐5p in breast cancer, colorectal cancer, and hepatocellular carcinoma, respectively, which enhance EC viability, migration and tube formation except in colorectal cancer to drive the process of carcinogenesis.…”

“…[ 175,224,226,239,225 ] In contrast, the remaining nine lncRNAs (GAS5, LINC01915, LINC00662, FAM225A, SNHG16, LINC00161, SNHG11, CCAT1, and UCA1), GAS5 with LINC01915 were downregulated in respiratory and colorectal cancer, [ 180,213 ] the left were upregulated in digestive cancer tissues and promoted oesophageal adenocarcinoma, hepatocellular and pancreatic cancer progression. [ 187,190,196,201,236,237,248 ]…”