Priming seed mitigates the effects of saline stress in soybean seedlings

Abstract

Seed germination and seedling growth are the most sensitive plant stages towards salinity. Various techniques can improve emergence and stand formation under salt conditions. Seeds priming is one of the most frequently used technique. Seed priming could develop different defence mechanisms of seeds against salinity stress. This experiment aims to examine the seed priming into potassium nitrate - KNO3 (1%), ascorbic acid – ASA (100mgl-1), and potassium chloride - KCl (1%) solutions, that may reduce harmful effects of salinity stress. Salinity was imposed by treatment of seeds with different concentrations of NaCl (0 (control), 50, 100, 200m MNaCl). All data were analysed statistically by three-way ANOVA. Individual testing of probability was carried out using Tukey’s method p £ 0.05 and p £ 0.01. Correlation dependence was determined using the Pearson correlation coefficient. Test results demonstrated positive effect of seed priming. This was showed by improved soybean seed quality and increased seed germination rate. The intensity of lipid peroxidation, free proline concentration and Na+ was decreased, while K+ in seedlings increased due to priming treatment. Priming of seeds into ASA solution resulted in increased vitamin C concentration, while priming into KNO3 and ÊCl solutions reduced the content of vitamin Ñ in soybean seedlings.

​Influence of Temperature and Salinity Stress on Seed Germination and Seedling Growth of Soybean (Glycine max L.)

“…As is known, some plants are moderately tolerant of saline conditions, while many plants are negatively affected by even low salt levels. Salinity negatively affects plant growth and productivity at all stages of development (Miladinov et al 2020).…”

Effects of foliar application of solutions of ascorbic acid, glycine betaine, salicylic acid on the yield and seed germination of soybean in South Eastern Europe conditions

“…In adverse conditions, a greater (but not significant) effect was achieved using ascorbic acid. Miladinov et al (2020) suggest that the beneficial effect of ascorbic acid on germination of soybean seeds is due to a decrease in lipid peroxidation intensity and increased free proline accumulation. Also, the beneficial effect of ascorbic acid can be attributed to its positive effect in the process of biosynthesis of gibberellin (Shu et al, 2013) and cytokinin (Huang et al, 2017) -hormones that have a stimulating effect on the seed germination process.…”

Priming with Humic Acid to Reverse Ageing Damage in Soybean [Glycine max (L.) Merrill.] Seeds

“…The current study proved that priming with HA for 5 h increased germination percentage, seedling quality and field establishment especially in water shortage conditions particularly when minimally aged seeds (due to deterioration in storage) were used. Although literature has shown a positive effect of various other priming agents on the improvement of soybean seeds [15][16][17], to date, there is no record of improvement for seeds that has been stored for more than six months. Additionally, all the priming agents used are chemical in nature, hence the use of humic acid may serve as an environmental and user-friendly priming agent.…”

“…Several seed priming techniques are used currently, such as water (hydro-priming), osmotic solutions (osmo-priming), inorganic salts (halo-priming), matric materials (matric-priming) and various natural extracts (live or decomposition materials from plant or animal parts) to enhance seed and seedling vigour. In previous studies, various solutions were suggested as priming agents for soybean such as polyethylene glycol (PEG) [15], salicylic acid (SA) [16], potassium nitrate (KNO 3 ), potassium chloride (KCl) and ascorbic acid (ASA) [17]. Although the above-mentioned chemicals have shown a positive effect, concerns of its use on the environment and the search for user-friendly seed priming agent for sustainability is on-going.…”