Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue

Abstract

In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (H2O2) or distilled water (H2Od),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM H2O2 or H2Od or used as controls, and cultured for one month, during which dark field and tilt illumination photos were taken. These were used to observe and quantify, time until cellular growth and confluence on the posterior capsule. After culture, histological sections were stained for H&E, α-SMA, Ki-67 and vimentin and evaluated. We prevented cellular growth in 50% of H2Od and 58% H2O2 of treated samples. The overall prevention of cell growth compared to cultured controls was significant for both treatments while there was no significant difference between them. In the cases where cellular growth was not prevented, both treatments significantly delay cellular growth. Until day 28 none of the treated samples of either type that had shown growth reached total confluence. All cultured controls reached total confluence before treated samples (median = day 11.5). Also, histologically, there was a clear morphological difference between cultured controls and treated samples.

Depletion of Myo/Nog Cells in the Lens Mitigates Posterior Capsule Opacification in Rabbits

“…13,20,[50][51][52] Cytotoxic drugs and chemicals that nonspecifically kill cells at the time of surgery and extensive removal of lens epithelial cells may cause the IOL to be more mobile, and drugs may diffuse to surrounding ocular tissues and initiate an inflammatory response to necrotic tissue. 47,[53][54][55][56][57][58] Currently, there is no effective method for predicting who will develop PCO or preventing its occurrence, and eradication of the disease remains an unmet goal in ophthalmology. Prevention of PCO is particularly important for insertion of shape-changing IOLs that accommodate for distance because fibrosis and contractions affect the flexibility of the lens capsule.…”

Role of Decorin in Posterior Capsule Opacification and Eye Lens Development

“…Currently, there is no effective method for predicting who will develop PCO or to prevent its occurrence, and eradication of the disease remains an unmet goal in ophthalmology. Certain molecules, such as cytotoxic chemicals, or distilled water may non-specifically induce cell death during cataract surgery and influence the surrounding ocular tissues [ 49 , 50 ]. Aberrant TGF β signaling plays a major role in the EMT of cells akin to its role in the development of human PCO.…”

Factors Affecting Posterior Capsule Opacification in the Development of Intraocular Lens Materials

“…At 9.3 days, the control IOLs showed full cell coverage whereas at this time point, the MTX-coated IOLs showed residual LECs were only visible at the outer edges and took 51.0-51.3 days to achieve full coverage. The application of ex vivo models is further supported by D'Antin [122] who used human eye donor tissue to investigate the treatment of hydrogen peroxide and distilled water to prevent cell proliferation within PCO development. The capsule bag-ciliary body complex was dissected from the donor eyes and transferred to sterile petri dishes onto a silicone ring mould where the iris was removed.…”