Prevalence of red and orange microbial complexes in endodontic-periodontal lesions: a systematic review and meta-analysis

Gambin, Diego José; Vitali, Filipe Colombo; Carli, João Paulo De; Mazzon, Ricardo Ruiz; Gomes, Brenda P. F. A.; Duque, Thais Mageste; Trentin, Micheline Sandini

doi:10.1007/s00784-021-04164-4

Cited by 14 publications

(14 citation statements)

References 65 publications

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“…There is moderate evidence that P. intermedia is a pathogen associated with periodontitis ( Gambin et al., 2021 ), but we found that its pathogenic component, LPS, promoted osteogenesis and upregulated expression of COL1A1, OCN and RUNX2 in vitro . Other scholars have also found that various components of bacteria have roles in promoting osteogenesis.…”

Section: Discussionmentioning

confidence: 58%

The oral microbiome in young women at different stages of periodontitis: Prevotella dominant in stage III periodontitis

Zhao

Feng

et al. 2022

Front. Cell. Infect. Microbiol.

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ObjectivePeriodontitis progression is related to the dynamic dysbiosis of oral microbiome. We identified the dominant bacteria and the potential pathway in young women with stage-III periodontitis.Materials and methodsSamples of subgingival plaque were collected from 26 young women with periodontitis (20 with stage-I and 6 with stage-III). Using 16S rRNA-sequencing, we determined the variation in oral bacterial communities of the two groups, and identified the dominant bacteria of each group. We used the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to evaluate the signaling pathways related to the difference in oral bacterial composition. The role of the dominant bacteria of stage-III periodontitis was investigated in vivo and in vitro using an endoplasmic reticulum stress inhibitor.ResultsYoung women with stage-I periodontitis had higher values for the Chao1 Index, Observed Species and Phylogenetic Diversity Whole Tree Index than those for women with stage-III periodontitis. β-diversity analyses revealed that samples could be divided into different groups according to the periodontitis stage. The most representative biomarkers of stage-III periodontitis in young women were bacteria of the phylum Bacteroidetes, its order, family and genera Bacteroidales, Prevotellaceae and Prevotella. The KEGG database revealed that the change in oral bacterial composition of young women with stage-III periodontitis may be related to protein processing in an endoplasmic reticulum pathway. Salubrinal (an endoplasmic reticulum stress regulator) controlled expression of Runx2, Col1a1, Ocn in mouse bone-marrow mesenchymal cells. Salubrinal administration showed that moderate endoplasmic reticulum stress inhibited alveolar bone loss in periodontitis induced by Prevotella intermedia lipopolysaccharide.ConclusionDifferences between periodontitis stages were noted and bacteria of Prevotella species were abundant in young women with stage-III periodontitis. This phenomenon was related to protein processing in an endoplasmic reticulum pathway.

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Section: Discussionmentioning

confidence: 58%

The oral microbiome in young women at different stages of periodontitis: Prevotella dominant in stage III periodontitis

Zhao

Feng

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Prevotella is a genus of Gram-negative bacteria and is one of the most important genera of bacteria in the oral cavity (Segata et al, 2012;Tett et al, 2021). Among the Prevotella species (including Prevotella and Prevotella_7), P. intermedia is part of the "orange complex" and is associated with chronic periodontitis, aggressive periodontitis, and gingivitis (Könönen and Müller, 2014;Gambin et al, 2021). As for another Prevotella soecies P. nigrescens, it was observed in in the subgingival plaque of periodontitis patients and induced host IL-1β production in dendritic cells (Jang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Among the Prevotella species (including Prevotella and Prevotella_7 ), P . intermedia is part of the “orange complex” and is associated with chronic periodontitis, aggressive periodontitis, and gingivitis ( Könönen and Müller, 2014 ; Gambin et al, 2021 ). As for another Prevotella soecies P .…”

Section: Discussionmentioning

confidence: 99%

Prevotella genus and its related NOD-like receptor signaling pathway in young males with stage III periodontitis

Zhao

Feng

et al. 2022

Front. Microbiol.

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BackgroundAs periodontitis progresses, the oral microbiota community changes dynamically. In this study, we evaluated the dominant bacteria and their roles in the potential pathway in young males with stage III periodontitis.Methods16S rRNA sequencing was performed to evaluate variations in the composition of oral bacteria between males with stage I and III periodontitis and identify the dominant bacteria of each group. Function prediction was obtained based on 16S rRNA sequencing data. The inhibitor of the predominant pathway for stage III periodontitis was used to investigate the role of the dominant bacteria in periodontitis in vivo and in vitro.ResultsChao1 index, Observed Species and Phylogenetic Diversity (PD) whole tree values were significantly higher in the stage III periodontitis group. β-diversity suggested that samples could be divided according to the stages of periodontitis. The dominant bacteria in stage III periodontitis were Prevotella, Prevotella_7, and Dialister, whereas that in stage I periodontitis was Cardiobacterium. KEGG analysis predicted that variations in the oral microbiome may be related to the NOD-like receptor signaling pathway. The inhibitor of this pathway, NOD-IN-1, decreased P. intermedia -induced Tnf-α mRNA expression and increased P. intermedia -induced Il-6 mRNA expression, consistent with the ELISA results. Immunohistochemistry confirmed the down-regulation of TNF-α and IL-6 expressions by NOD-IN-1 in P. intermedia–induced periodontitis.ConclusionThe composition of the oral bacteria in young males varied according to the stage of periodontitis. The species richness of oral microtia was greater in young males with stage III periodontitis than those with stage I periodontitis. Prevotella was the dominant bacteria in young males with stage III periodontitis, and inhibition of the NOD-like receptor signaling pathway can decrease the periodontal inflammation induced by P. intermedia.

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“…The gingival epithelium not only acts as a physical barrier to microorganisms in the host immune defense but also recognizes microbiota-associated molecular patterns (MAMPs) by expressing various pattern recognition receptors (PRRs) and reacts with MAMPs by secreting various cytokines and chemical factors such as IL-8 and antibacterial peptides, which plays an active biological barrier role in host immune recognition and initiation and actively participates in the host’s innate immune response and acquired immune response [ 13 , 14 , 15 ]. Despite the difficulty in defining the mechanisms of periodontitis, it is certain that the immune status of the local periodontal microenvironment in healthy tissues and periodontitis tissues are different, in which several types of host immune cells such as macrophages, T cells and host stem cells are involved [ 16 ] (shown schematically in Figure 1 ). Therefore, it is necessary to consider changes in the periodontal microenvironment and host immune regulation in the process of periodontal regeneration.…”

Section: Introductionmentioning

confidence: 99%

Regulation of the Host Immune Microenvironment in Periodontitis and Periodontal Bone Remodeling

Han

Liu

et al. 2023

IJMS

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The periodontal immune microenvironment is a delicate regulatory system that involves a variety of host immune cells including neutrophils, macrophages, T cells, dendritic cells and mesenchymal stem cells. The dysfunction or overactivation of any kind of local cells, and eventually the imbalance of the entire molecular regulatory network, leads to periodontal inflammation and tissue destruction. In this review, the basic characteristics of various host cells in the periodontal immune microenvironment and the regulatory network mechanism of host cells involved in the pathogenesis of periodontitis and periodontal bone remodeling are summarized, with emphasis on the immune regulatory network that regulates the periodontal microenvironment and maintains a dynamic balance. Future strategies for the clinical treatment of periodontitis and periodontal tissue regeneration need to develop new targeted synergistic drugs and/or novel technologies to clarify the regulatory mechanism of the local microenvironment. This review aims to provide clues and a theoretical basis for future research in this field.

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Prevalence of red and orange microbial complexes in endodontic-periodontal lesions: a systematic review and meta-analysis

Cited by 14 publications

References 65 publications

The oral microbiome in young women at different stages of periodontitis: Prevotella dominant in stage III periodontitis

The oral microbiome in young women at different stages of periodontitis: Prevotella dominant in stage III periodontitis

Prevotella genus and its related NOD-like receptor signaling pathway in young males with stage III periodontitis

Regulation of the Host Immune Microenvironment in Periodontitis and Periodontal Bone Remodeling

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