Prevalence of mycoplasmas in the respiratory tracts of calves in Brazil

Marques, Lucas Miranda; Buzinhani, Melissa; Oliveira, Renata Cristina Gobbi de; Yamaguti, Maurício; Ferreira, Juliana Bonin; Neto, R. L.; Timenetsky, Jorge

doi:10.1136/vr.161.20.699

Cited by 10 publications

(16 citation statements)

References 13 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our data are in agreement with a previous study, which also described the increased occurrence of M. dispar in both healthy and BRD groups, especially in the latter group. Two other studies also detected a high occurrence of M. dispar in BRD cattle compared to healthy cattle . Mycoplasma mycoides subsp.…”

Section: Discussionsupporting

confidence: 93%

“…mycoides SC is the etiologic agent of contagious bovine pleuropneumonia, and it is considered the most important mycoplasma species related to BRD . In our study, this species was not detected, and this result is in accordance with other Brazilian studies . M. bovis is another important mycoplasma related to BRD .…”

Section: Discussioncontrasting

confidence: 74%

“…Ureaplasma diversum , Acholeplasma spp. and other mycoplasma species such as M. bovirhinis, M. alkalensis, and M. arginini have been detected in the bovine respiratory tract . The genus Mycoplasma has several species, and culture‐independent techniques are indispensable to determine all species present in the respiratory tract.…”

Section: Discussionmentioning

confidence: 97%

“…Similarly, Mollicutes have been reported frequently in more BRD calves (90.96%; 53%) compared to healthy calves (52.05%; 23%) . Mollicutes are well characterized as part of t he bovine respiratory tract microbiota but several species have been described as etiologic agents of respiratory diseases…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation ofMollicutesMicroorganisms in Respiratory Disease of Cattle and Their Relationship to Clinical Signs

Tortorelli

Gaeta

Ribeiro

et al. 2017

Veterinary Internal Medicne

Self Cite

View full text Add to dashboard Cite

BackgroundBovine respiratory disease (BRD) is an important problem in cattle production that is responsible for economic losses in dairy herds. Mycoplasma spp. are described as an important etiological agent of BRD.HypothesisTo evaluate the occurrence of the most important mycoplasmas in the lower respiratory tract of healthy and BRD cattle in relationship to clinical signs of BRD.AnimalsSixty young dairy cattle were classified as healthy (n = 32) or cattle showing clinical signs of BRD (n = 28).MethodsTracheal lavage samples were collected and added to tubes containing Hayflick media. Mycoplasma spp. were identified by the presence of “fried egg” like colonies, biochemical tests and polymerase chain reaction (PCR). Occurrence of Mollicutes, M. bovis, M. mycoides subsp. mycoides SC and M. dispar was evaluated. The association between clinical signs of BRD and the presence of Mycoplasma spp. also was evaluated.ResultsColonies were obtained from a 1‐year‐old BRD calf only. However, species identification was not possible. Mollicutes (P = .035) and M. dispar (P = .036) were more common in BRD cattle. The relationship between Mollicutes and crackle (P = .057) was not significant. M. dispar was associated to tachypnea (P = .045) and mixed dyspnea (P = .003). Relationships to heart rate (P = .062) and crackle (P = .062) were not significant.Conclusions and clinical importanceThe results confirmed the importance of mycoplasma as an etiologic agent of BRD and suggested M. dispar as part of the respiratory microbiota and its possible role in the development of BRD.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 74%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation ofMollicutesMicroorganisms in Respiratory Disease of Cattle and Their Relationship to Clinical Signs

Tortorelli

Gaeta

Ribeiro

et al. 2017

Veterinary Internal Medicne

Self Cite

View full text Add to dashboard Cite

show abstract

“…The dairy farms from which the mycoplasmas were detected all had clinical evidence of disease based on the survey completed in a previous study, and 9 of the farms that elected to do individual cow testing during the subsequent year as a follow up to the bulk tank identification detected the disease in individual cows in their herds ). The occurrence of mixed mycoplasma outbreaks has been documented (Mackie et al, 2000;González and Wilson, 2003) and multiple species of mycoplasmas can be isolated from normal and pneumonic calves (Thomas et al, 2002;Marques et al, 2007). However, the frequency of the simultaneous occurrence of multiple species of mycoplasmas on farms that are not experiencing an outbreak of mycoplasma mastitis is unknown.…”

Section: Discussionmentioning

confidence: 99%

Detection of multiple Mycoplasma species in bulk tank milk samples using real-time PCR and conventional culture and comparison of test sensitivities

Justice-Allen

Trujillo

Goodell³

et al. 2011

Journal of Dairy Science

View full text Add to dashboard Cite

The objective of this study was to further validate a SYBR PCR protocol for Mycoplasma spp. by comparing it with standard microbial culture in the detection of Mycoplasma spp. in bulk tank milk samples. Additionally, we identified Mycoplasma spp. present by analysis of PCR-generated amplicons [dissociation (melt) temperature (T(m)), length, and DNA sequence]. The research presented herein tests the hypothesis that the SYBR PCR protocol is as sensitive as conventional culture for the detection of Mycoplasma spp. in bulk tank milk samples. Mycoplasmas cause several important disease syndromes in cattle, including mastitis in dairy cows. The standard diagnostic method at the herd level has been microbial isolation of mycoplasmas on 1 of several specialized media and speciation through biochemical or immunological techniques; repeated sampling schemes are recommended. The development of a real-time SYBR PCR protocol offers advantages in decrease of time to detection, cost, and complexity. The T(m) of the double-stranded DNA generated from the PCR reaction was used to detect the presence of and tentatively identify the species of mycoplasmas other than Mycoplasma bovis. In the SYBR PCR protocol, the presence of multiple species of mycoplasmas is indicated by an atypical dissociation curve. Gel electrophoresis and sequencing of the amplicons was used to confirm the mycoplasma species present when a non-M. bovis organism was detected (T(m) not equal to M. bovis) and used to identify all the mycoplasma species present for the samples with atypical dissociation curves. Mycoplasma bovis was identified in 83% of SYBR PCR mycoplasma-positive bulk tank samples. Another mycoplasma was identified either alone or in addition to M. bovis in 25% of SYBR PCR mycoplasma-positive bulk tank milk samples. Four species of mycoplasma other than M. bovis (Mycoplasma alkalescens, Mycoplasma arginini, Mycoplasma bovigenitalium, and Mycoplasma gateae) were identified in bulk tank milk samples tested with this method. Five farms had 2 mycoplasma species occurring at different times in their bulk tanks. Two mycoplasma species were identified in the same bulk tank sample in 7 instances on 2 farms. The finding of multiple Mycoplasma spp. coexisting on a farm and even in the same bulk tank milk sample indicates that the clinical significance of multiple mycoplasma species in the pathology of intramammary infections should be investigated further. In comparison with conventional culture, the SYBR PCR protocol was slightly (but not statistically significantly) more sensitive in the detection of mycoplasmas in bulk tank milk.

show abstract

Determination of bacterial aetiologic factor on tracheobronchial lavage in relation to clinical signs of bovine respiratory disease

Oliveira

Gaeta

Ribeiro

et al. 2016

Journal of Medical Microbiology

View full text Add to dashboard Cite

This study aimed to determine the occurrence of Mannheimiahaemolytica, Pasteurella multocida and Mycoplasma spp., in relation to clinical signs of respiratory disease. Tracheobronchial lavage samples were collected from 96 (healthy and unhealthy) cattle in the State of São Paulo, Brazil. Mycoplasma spp. (12.5 %) and Pasteurellamultocida (15.50 %) were the most prevalent species. Bacillus sp., Staphylococcus sp., Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa and Klebsiella pneumoniae were also isolated. Mollicutes (70.83 %), Mycoplasmabovis (2.94 %) and Mycoplasma dispar (38.23 %) were identified using conventional PCR. Submassive sound on acoustic percussion of the thorax was associated with the absence of Mollicutes (P=0.025). Whistling (P=0.076) and coarse crackle (P=0.046) were associated with the absence of Mycoplasma dispar. Clear sound on acoustic percussion of the thorax was associated with the absence of Mycoplasmabovis (P=0.007). Coughing was associated with the presence of Pasteurellamultocida [P=0.035; confidence interval (CI), 1.12-26.89], but its absence was associated with mucopurulent (P=0.0215; CI, 1.55-34.5) and mucoid nasal discharge (P=0.068; CI, 19-28.5), submassive sound (P=0.031; CI, 1.23-75.5), fine crackle (P=0.058; CI, 1.23-20.1) and coarse crackle (P=0.046; CI, 2.38-70.8). The high prevalence of Pasteurella multocida and Mycoplasma spp. in unhealthy calves increases the importance of these micro-organisms in the pathogenesis of respiratory diseases. This study increases the information about the role of Mycoplasma dispar in respiratory diseases. Differences in some species in relation to clinical signs can be applied as a presumptive diagnosis.

show abstract

Prevalence of mycoplasmas in the respiratory tracts of calves in Brazil

Cited by 10 publications

References 13 publications

Evaluation ofMollicutesMicroorganisms in Respiratory Disease of Cattle and Their Relationship to Clinical Signs

Evaluation ofMollicutesMicroorganisms in Respiratory Disease of Cattle and Their Relationship to Clinical Signs

Detection of multiple Mycoplasma species in bulk tank milk samples using real-time PCR and conventional culture and comparison of test sensitivities

Determination of bacterial aetiologic factor on tracheobronchial lavage in relation to clinical signs of bovine respiratory disease

Contact Info

Product

Resources

About