Prevalence of human papillomavirus genotypes in cytologic abnormalities from unvaccinated women living in north-western Spain

Otero-Motta, Ana Pastora; Ordóñez, José Luis; González-Celador, Rafael; Rivas, Belen; Macías, María Del Carmen García; Bullón, A; Abad, María del Mar

doi:10.1111/j.1600-0463.2010.02711.x

Cited by 17 publications

(14 citation statements)

References 36 publications

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“…The presence of co-infection was more frequent in LSIL cases (45%), followed ASCUS (36%) and NC (25%), whereas a decreased frequency of co-infection was found in HSILs (20%). Similar to other Spanish studies [15,26], in our series, ASCUS/LSILs were associated with co-infections, whereas HSILs were associated with single infection (p < 0.01). It is still not clear whether co-infection with several types increases the risk of progression.…”

Section: Discussionsupporting

confidence: 91%

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Human papillomavirus genotype distribution in Madrid and correlation with cytological data

et al. 2011

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BackgroundCervical cancer is the second most common cancer in women worldwide. Infection with certain human papillomavirus (HPV) genotypes is the most important risk factor associated with cervical cancer. This study analysed the distribution of type-specific HPV infection among women with normal and abnormal cytology, to assess the potential benefit of prophylaxis with anti-HPV vaccines.MethodsCervical samples of 2,461 women (median age 34 years; range 15-75) from the centre of Spain were tested for HPV DNA. These included 1,656 samples with normal cytology (NC), 336 with atypical squamous cells of undetermined significance (ASCUS), 387 low-grade squamous intraepithelial lesions (LSILs), and 82 high-grade squamous intraepithelial lesions (HSILs). HPV detection and genotyping were performed by PCR using 5'-biotinylated MY09/11 consensus primers, and reverse dot blot hybridisation.ResultsHPV infection was detected in 1,062 women (43.2%). Out of these, 334 (31%) samples had normal cytology and 728 (69%) showed some cytological abnormality: 284 (27%) ASCUS, 365 (34%) LSILs, and 79 (8%) HSILs. The most common genotype found was HPV 16 (28%) with the following distribution: 21% in NC samples, 31% in ASCUS, 26% in LSILs, and 51% in HSILs. HPV 53 was the second most frequent (16%): 16% in NC, 16% in ASCUS, 19% in LSILs, and 5% in HSILs. The third genotype was HPV 31 (12%): 10% in NC, 11% in ASCUS, 14% in LSILs, and 11% in HSILs. Co-infections were found in 366 samples (34%). In 25%, 36%, 45% and 20% of samples with NC, ASCUS, LSIL and HSIL, respectively, more than one genotype was found.ConclusionsHPV 16 was the most frequent genotype in our area, followed by HPV 53 and 31, with a low prevalence of HPV 18 even in HSILs. The frequency of genotypes 16, 52 and 58 increased significantly from ASCUS to HSILs. Although a vaccine against HPV 16 and 18 could theoretically prevent approximately 50% of HSILs, genotypes not covered by the vaccine are frequent in our population. Knowledge of the epidemiological distribution is necessary to predict the effect of vaccines on incidence of infection and evaluate cross-protection from current vaccines against infection with other types.

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Section: Discussionsupporting

confidence: 91%

“…Therefore, obtaining a solid knowledge of genotype HPV distribution is becoming imperative. Multiple types of HPV have been described in up to 50% of NC and LSIL cases in the population in Northwest Spain [15], in accordance with other studies [30]. …”

Section: Discussionsupporting

confidence: 85%

Human papillomavirus genotype distribution in Madrid and correlation with cytological data

et al. 2011

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“…Our results fall within this range. This prevalence of multiple HPV infection is higher than found in other studies conducted in Spain in which the values range between 4.7% and 52.1% [6,29,30,34,36,38,57]. Furthermore, in a study of patients with abnormal cytology obtained a higher value (58.1%) [35] than found in this study.…”

Section: Discussioncontrasting

confidence: 55%

“…In this study, HPV-51 (7.8%), -52 (5.2%) and -66 (2.6%) were detected. These viral types appear to be common in the Spanish population [6,18,[35][36][37][38], and these HPV specific-genotypes were also detected in studies around the world. The proportions detected in these studies are diverse, being as high as 17.5%, 12.0%, and 8.1% respectively [35], while in other study [38] found similar rates to those of our study: 8.0%, 3.8%, and 7.1%, respectively.…”

Section: Discussionmentioning

confidence: 95%

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Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Méndez¹

2013

SOJ Microbiol Infect Dis

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BackgroundCervical cancer is the second most common cancer in women worldwide and the most common cause of mortality in underdeveloped and developing countries [1,2]. Persistent infection with certain oncogenic high-risk (HR) types of Human Papillomavirus (HPV) is the required factor for the development of invasive cervical cancer and precursor lesions [3][4][5][6].Overall, the prevalence and distribution of HPV infection in cervical lesions has been determined in many geographical regions of the world, this can vary depending on several factors including epidemiological differences in the populations studied and the methodology used for molecular detection and typing of HPV DNA [7]. Over 120 types of HPV have been classified as either low (LR-HPV) or high risk (HR-HPV), according to their oncogenic potential [8,9]. Within the high-risk genotypes, HPV types 16 and HPV 18 are most often associated with cancer and squamous intraepithelial lesion [5,6,10,11,12]. Nevertheless, the risk of neoplasia for other types of HPV as well as for multiple HPV infection has not yet been established.Because current strategies for the prevention of cervical cancer and their precancerous lesions are based on the HPV genotyping [13] and prophylactic vaccines [14][15][16], it seems necessary to determine the types of HPV most commonly associated with malignant cell transformation in different geographical areas in order to establish effective preventive measures according to the epidemiology of the population. This study was to know the prevalence and distribution of cervical HPV infection in women attending routine cervical cancer screening in the Valencia region (Spain). Study designLiquid-based cytology (LBC) of cervical specimens was collected during routine screening visits, between June 20, 2011 and September 9, 2011 in the Clinical Hospital of Valencia, Spain. LBC samples were processed using the Thin Prep® 5000 processor (Madrid, Spain). For HPV detection and genotyping, we used the residual cervical sample material available from the same Thin Prep Pap test vial previously used for cytological screening. DNA was extracted from the residual materials of LBC by QIAamp® DNA mini kit (IZASA, Valencia, Spain), according to the manufacturer's instructions. HPV testing was performed by INNO-Lipa HPV genotyping Extra Reverse Hybridization Line Probe Assay kit (Innogenetics®, Barcelona, Spain), according to the manufacturer's instructions. Briefly, HPV detection and genotyping was based on PCR amplification of a 65pb fragment, within the L1 region of the HPV genome, using broad-spectrum SPF10 biotinylated primers. PCR was performed in a final AbstractBackground: Infection with human papillomavirus (HPV) has been identified as the primary cause of cervical cancer. Persistent infection with high risk-HPV is the required factor for the development of cervical cancer.

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HPV Infection: Pathogenesis and Detection

Aggarwal

2019

Preventive Oncology for the Gynecologist

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Prevalence of human papillomavirus genotypes in cytologic abnormalities from unvaccinated women living in north-western Spain

Cited by 17 publications

References 36 publications

Human papillomavirus genotype distribution in Madrid and correlation with cytological data

Human papillomavirus genotype distribution in Madrid and correlation with cytological data

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

HPV Infection: Pathogenesis and Detection

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