Prevalence of high‐risk human papilloma virus in liquid‐based cervical samples from Turkish women with normal and abnormal cytology

Abstract

Background: The aim of this study was to investigate the prevalence of human papilloma virus (HPV) infection from Turkish women with normal and abnormal cytology.Methods: Our study population consisted of 1252 women with normal and abnormal cytology.In our clinic, cervical cancer screening was performed by liquid-based cytology testing (ThinPrep Pap Test). Reflex high-risk (HR) HPV testing (Cervista HPV HR and HPV 16/18) was performed in cases with abnormal cytology (n = 330). HR HPV testing was performed to 922 cases with normal cytology.Results: HR HPV was detected in 18.7% of negative for intraepithelial lesion or malignancy (NILM) samples, and in 79.7% of abnormal cytology cases. HPV types 16 and/or 18 were found in 18.6% and 35.3% of normal cytology cases and abnormal cytology cases, respectively. Of all 435 HR HPV-positive samples, HPV type 16 and/or 18 prevalence was significantly higher in cases with more severe cytological abnormalities. Conclusions:The HPV prevalence among Turkish women with normal and abnormal cytology identified in this study largely concurs with those in other studies throughout the world. HPV types 16 and/or 18 were detected significantly in normal cytology cases in our study. We also found that, as has been previously demonstrated, certain HPV genotypes (16/18) are associated with more severe pathologies. However, larger epidemiological studies in different regions of Turkey are needed to evaluate the accurate prevalence of HPV infection throughout the country. K E Y W O R D S cervical cytology, human papilloma virus, Turkey ;47:100-104.women and to investigate the role of HR HPV infections in women with normal and abnormal cervical cytology. | MATERIALS AND METHODS | Study population and specimen preparationThe study protocol was approved by the Ethics Committee of the Umraniye Education and Research Hospital. According to ethical standards in our country, an informed patient consent is not necessary due to the design of the retrospective study (the study database was created from a retrospective file review). Umraniye is a district of Istanbul which has received migrants from rural areas of Anatolia with low socio-economic level. The Department of Pathology's archives from November 2013 to May 2015 in the Umraniye Education and Research Hospital were searched to identify all women with cervical cytology records. Opportunistic cervical cancer screening in our hospital was performed by liquid-based cytology testing (ThinPrep Pap Test). Reflex HR HPV testing (Cervista HPV HR and Cervista HPV 16/18) was performed in all cases with abnormal cytology. Cases were selected if both molecular analysis and cytology were available. HR HPV testing was performed to 922 cases with normal cytology due to the patients' request and 412 cases with abnormal cytology. Cytological samples were classified according to the Bethesda 2001/2014 system as negative for intraepithelial lesion or malignancy (NILM), atypical squamous cells of undetermined significance (ASC-US), atypical squamous cells, cannot e...

The prevalence and distribution of human papillomavirus in 4267 Turkish women with or without cervical lesions: A hospital‐based study

“…Seneldir et al detected 34.7% HR‐HPV in their hospital‐based study. Among HR‐HPV positive samples, 25.3% were HPV type 16, 3.9% were type 18% and 71.3% were OHR‐HPV 25 . Alan et al detected 34.2% HR‐HPV in their hospital‐based study 21 .…”

Cytomorphometry and morphology analysis of human papillomavirus type 16 in liquid‐based cervical cytology samples

“…HPV is an important virus that causes benign and malignant lesions in women and men 4‐6 . Wang et al indicated that the younger women with ASCUS and positive high risk for HPV testing have the highest risk of developing high‐grade CIN lesions 29 .…”

“…Cervical cancer is the third most common cancer among women worldwide 1,2 According to data from the Ministry of Health registry cervical cancer is the 10th most common cancer among females in Turkey 1,3 . HPV 16 and HPV 18 are the most prevalent and carcinogenic HPV types and account for approximately 55% to 60% and 10% to 15% of all cervical cancer cases, respectively 4‐6 . HPV 16 is present in the human population in various molecular variants.…”

Comparison of biopsy results of HPV 16/18 and non-16/18 HPV positive patients with a normal PAP test, a tertiary center experience

“…Cervical cancer is a public health problem worldwide, with an estimated 528,000 new cases and 266,000 deaths per year [10,11]. In Turkey, cervical cancer is the 10 th most observed cancer [12]. Mortality and morbidity due to cervical cancer mostly affects low and middle-income countries [10].…”