Prevalence, molecular characterization and antimicrobial resistance of Salmonella serovars isolated from northwestern Spanish broiler flocks (2011–2015)

Abstract

The present study investigated the prevalence, antimicrobial resistance to twenty antibiotics, and class 1 integron and virulence genes of Salmonella isolated from poultry houses of broilers in northwestern Spain between 2011 and 2015. Strains were classified to the serotype level using the Kauffman-White typing scheme and subtyping with enterobacterial repetitive intergenic consensus PCR. The prevalence of Salmonella spp. was 1.02%. Sixteen different serotypes were found, with S. typhimurium and S. arizonae 48:z4, z23:- being the most prevalent. A total of 59.70% of strains were resistant to at least one, and 19.70% were resistant to multiple drugs. All Salmonella spp. were susceptible to cefotaxime, ciprofloxacin, gentamicin, kanamycin, levofloxacin, neomycin, and trimethoprim. The highest level of resistance was to sulfamethoxazole (40.29%), doxycycline (17.91%), and nalidixic acid (17.91%). None of the isolates carried class 1 integron and only isolates of S. enterica subspecies enterica were positive for all virulence factors tested, whereas S. arizonae lacked genes related to replication and invasion in nonphagocytic cells. This study demonstrates that the prevalence and antimicrobial resistance of Salmonella spp. in poultry houses of broilers of northwestern Spain is low compared with those found in other studies and in other steps of the food chain.

Evaluation of the efficiency of using Salmonella Kentucky and Escherichia coli O119 bacteriophages in the treatment and prevention of salmonellosis and colibacillosis in broiler chickens

“…Our results showed a higher prevalence of Salmonella infection than those reported in previous studies (Lamas et al . 2016; Mahmoud et al . 2018).…”

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

“…These subspecies are usually found in cold-blooded animals and the main route of transmission to other animals is the consumption of their meat or the use of these animals as pets in the case of humans (Lamas et al, 2018). However, some serotypes from these subspecies, specially arizonae, diarizonae and salamanae, are also adapted to warm-blooded animals, either wild mammals and birds or domestic animals (Bonke et al, 2012;Botti et al, 2013;Chiari et al, 2013;Evangelopoulou, Kritas, Govaris, & Burriel, 2014;Lamas et al, 2016). Two studies performed in Italy described similar results regarding the relative abundance of subspecies found in wild boars.…”

Presencia y Resistencia a los Antimicrobianos de serovariedades de Salmonella enterica aisladas en una empresa avícola integrada del Ecuador

“…resultados de la presencia de Salmonella en las heces de pollos son similares a los reportados en Colombia en donde se detectó una prevalencia de 65% (n= 315) (Donado-Godoy et al 2012). Sin embargo, en China este microorganismo fue aislado del 11.4% (n=290) de muestras de ciegos y en España la prevalencia del patógeno en granjas de pollos de engorde fue del 1.02% (n=6577) (Cui et al 2016, Lamas et al 2016. En Estados Unidos Salmonella se detectó en el 43.1% (n=109) y 13.6% (n=220) de muestras de hisopado de barrido y heces, respectivamente (Berghaus et al 2013).…”