Prevalence, Molecular Characterization and Antibiotic Susceptibility of <scp><i>E</i></scp><i>scherichia Coli</i> Isolated from Duck Meat and Giblets

Darwish, Wageh Sobhy; Eldin, Walaa Fathy Saad; Eldesoky, Kamal I.

doi:10.1111/jfs.12189

Cited by 22 publications

(22 citation statements)

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“…It might also indicate cross‐contamination of the meat from the workers’ hands, cutting boards, water, and knives used during slaughtering, evisceration and processing of the camel meat and offal. Coliform bacteria are considered as significant microbiological sanitary indicators, which emphasizes hygiene in all steps of preparation and handling of food subjects (Darwish et al, 2015). The recorded mean values of TEC were 6.50 ± 0.18, 5.50 ± 0.20, 4.20 ± 0.18, 3.50 ± 0.21, 3.20 ± 0.33, and 2.70 ± 0.20 log CFU/g in the examined duodenum, rumen, lungs, kidneys, liver, and muscle, respectively (Figure 1c).…”

Section: Resultsmentioning

confidence: 99%

“…The high content of protein and moisture are considered as ideal factors that enhance microbial spoilage of the meat and offal. In addition, poor hygienic measures adopted during slaughtering, evisceration, distribution, and storage of the meat and offal are additional factors for the rapid onset of the microbial spoilage of the meat (Darwish, Eldin, & Eldesoky, 2015). However, there is a clear lack of information about the microbial quality of camel meat and offal in the local markets of Egypt.…”

Section: Introductionmentioning

confidence: 99%

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Microbial quality and formation of biogenic amines in the meat and edible offal of Camelus dromedaries with a protection trial using gingerol and nisin

Tang

Darwish

El‐Ghareeb

et al. 2020

Food Science & Nutrition

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This study aimed firstly at the investigation of the microbial status of the camel meat and edible offal including liver, kidneys, lungs, rumen, and duodenum distributed at local markets of Egypt. Total plate count, total psychrophilic counts, total Enterobacteriaceae count, the most probable number of coliforms, and total mold counts were monitored at the collected samples. The produced biogenic amines (BA) in the camel meat and offal were further estimated. An experimental trial to investigate the antimicrobial potentials of either nisin, gingerol, or an equal mixture of both using camel muscle as a food matrix was conducted. The achieved results declared a high microbial load in the camel meat and the offal. Duodenum and rumen had the highest microbial counts followed by lungs, kidneys, liver, and muscle, respectively.Similarly, duodenum and rumen had the highest levels of BA, including tyramine, spermine, putrescine, cadaverine, and histamine. Both of nisin and ginger showed significant antimicrobial properties in a concentration-dependent manner. Thus, efficient hygienic measures during the handling of camel meat are highly recommended.In addition, using nisin, gingerol, or a mixture of both is an efficient strategy for improving the microbiological quality of the camel meat. K E Y W O R D Scamel meat, gingerol, microbial quality, nisin, offal | 2095 TANG eT Al.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial quality and formation of biogenic amines in the meat and edible offal of Camelus dromedaries with a protection trial using gingerol and nisin

Tang

Darwish

El‐Ghareeb

et al. 2020

Food Science & Nutrition

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“…In another study, conducted by Darwish et al (19) concerning the antibiotic susceptibilities of E. coli serogroups, E. coli O86 was highly sensitive to cefotaxime, followed by ciprofloxacin and amoxicillin, whereas E. coli O127 was highly susceptible to cefotaxime but resistant to amoxicillin and showed weak sensitivity to other antibiotics tested. In the same study (19), E. coli O114 was highly susceptible to danofloxacin and norfloxacin.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance profiles and virulence factors of Escherichia coliO157 collected from a poultry processing plant*

Hızlısoy¹,

Al²,

Onmaz³

et al. 2017

Turk J Vet Anim Sci

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IntroductionEscherichia coli O157 and other enterohemorrhagic E. coli (EHEC) are a worldwide threat to public health (1). Mostly in developed countries, E. coli O157 has been recognized as a cause of serious clinical symptoms such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) characterized by thrombocytopenia, hemolytic anemia, and nephropathy (2). Cattle are the primary reservoir and asymptomatic carriers of E. coli O157. The bacteria were spread via cattle faces (3). Most outbreaks of EHEC infections have been associated with the consumption of uncooked meat and meat products, unpasteurized dairy products, and feces-contaminated vegetables and water (2-4).E. coli O157, a member of EHEC, has important virulence factors such as shiga toxins (stx1 and stx2), enterohemolysin (hlyA), and intimin (eaeA). Shiga toxinproducing E. coli (STEC) can cause HC and HUS (5,6) due to the inhibiting protein synthesis, eukaryotic cell injury, and death (7). Intimin, encoded by the eaeA gene, is able to adhere to intestinal mucosa and cause intestinal lesions (5,7). In addition, enterohemolysin, encoded by hlyA, leads to lysis of the erythrocytes, which may contribute to the iron intake of the bacterium to live in the intestine (4).The frequency of antimicrobial resistance in foodborne pathogens such as E. coli, Salmonella spp., and Campylobacter spp. has recently increased. The misuse of antimicrobial agents for farming and therapeutic purpose in animals and humans is the main cause of transmission of antibiotic-resistant strains, which are very difficult to treat with commonly used antibiotics, to humans via the food supply (8).The aim of this study was to investigate the potential risk of public health by determining the prevalence of E. coli O157:H7 and identifying the virulence genes from The aims of this study were to investigate the prevalence of E. coli O157 and to identify virulence genes and also to determine the antibiotic susceptibility of the isolates in different poultry-originated materials.

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“…Positive results of fecal coliform or E.coli in the samples may indicate the existence of other pathogenic organisms, thus reflecting the potential health risks for individuals being exposed to samples. Contamination of ready to eat food is attributed to post-processing contamination due to many reasons such as unhygienic practices during food handling, lack of personal hygiene, unhygienic kitchen, utensils, equipment and packaging (Darwish et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial DNA was extracted from each of glycerol stock serodiagnosed E. coli according to the method described before (Darwish et al, 2015).…”

Section: Dna Preparationmentioning

confidence: 99%

The bacteriological status of retail cheese in Zagazig city, Egypt

Elbadry

Raslan

2016

Benha Veterinary Medical Journal

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Cheese is one of the most popular dairy products in Egypt. It supplies the body with protein, fat, vitamins and minerals. Cheese may be exposed to bacterial contamination during manufacture, distribution and/or storage. This study was undertaken to investigate the bacteriological status of different cheese types marketed in Zagazig city, Egypt. The obtained results showed that Roumy cheese had the highest total mesophilic and psychrophilic counts followed by white, soft and cheddar cheese types. Furthermore, the coliforms count and prevalence of different serotypes of E. coli in cheese samples were detected. In addition, the expression of shiga-toxin producing genes (stx1 & stx2) in the identified E. coli serotypes was screened using multiplex PCR. The obtained results revealed that only E. coli O127:H6 had expressed both stx1 & stx2 toxin producing genes, while E. coli O111:H4 had expressed only stx1 gene, however, both E. coli O124: H and O55:H7 had expressed stx2 gene. The public health importance of shiga-toxin producing E. coli was detected.

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Prevalence, Molecular Characterization and Antibiotic Susceptibility of Escherichia Coli Isolated from Duck Meat and Giblets

Cited by 22 publications

References 20 publications

Microbial quality and formation of biogenic amines in the meat and edible offal of Camelus dromedaries with a protection trial using gingerol and nisin

Microbial quality and formation of biogenic amines in the meat and edible offal of Camelus dromedaries with a protection trial using gingerol and nisin

Antimicrobial resistance profiles and virulence factors of Escherichia coliO157 collected from a poultry processing plant*

The bacteriological status of retail cheese in Zagazig city, Egypt

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