Prevalence and molecular characterization of plasmidmediated beta-lactamase genes among nosocomial &lt;i&gt;Staphylococcus aureus&lt;/i&gt; isolated in Taiwan

Yang, Chih‐Jen; Lee, Shaoyung; Pan, Hao-Yun; Su, Pai-Wei; Chuang, Li‐Yeh

doi:10.4314/tjpr.v16i1.20

Cited by 2 publications

(3 citation statements)

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“…All samples from poultry and layer farm have blaTEM gene. The similar result showed in Iran and Taiwan where 100% beta lactamase resistant strain have a blaTEM gene (Yang et al, 2017;Nouri-Gharajalar et al, 2019). BlaTEM gene encodes most encountered bacteria â-lactamase (Xu et al, 2014).…”

Section: Resultssupporting

confidence: 78%

“…BlaTEM gene encodes most encountered bacteria â-lactamase (Xu et al, 2014). Most strains, which have blaTEM gene, exhibited a high degree of resistance to â-lactam antibiotics in China and Taiwan (Yang et al, 2017). Modifcation of penicillin-binding protein (PLP2a) induces inactivity of penicillin and other beta lactam antibiotics; and it will develop cross-resistance of multiple antibiotics (Bounar-Kechih et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

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Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor

et al. 2021

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Chicken is one of the important protein source in Indonesia. Moreover, the largest population of chicken layer and poultry in Indonesia is known situated at West Java province with Bogor manicipality as the main producer. The aims of this study were to determine the antibiotic resistance pattern of Staphylococcus aureus isolated from poultry and layer farm in Bogor. The study also identified gene encoded the resistance. Cloacal swab samples were collected from chicken broiler and layer farm in Bogor manicipality. The samples were then cultured in Mannitol Salt Agar (MSA) medium to obtain S. aureus. Suspected colony was then confirmed by biochemical test. Positive strains were tested against several antibiotics and the diameter of clear zone arround of colony was interpreted based on Clinical and Laboratory Standard Institute. Furthermore, the DNA from resistant strains were then extracted, followed by detection of the resistance gene by using polymerase chain reaction (PCR) method. A total of 14 isolates of S. aureus were positive from poultry farm, and 15 isolates from layer farm. Most of all were resistant to tetracycline, ampicillin, oxytetracycline, erythromycin and nalidixic acid. On the other hands, several strains were sensitive to gentamycin and chloramphenicol. The study showed 28 isolates out of them were multi-drug resistant. Resistant gene such as blaTEM, gyrA and tetA were also identified in some isolates except for ErmB gene which was found in isolates originated from poultry farm. In conclussion, S. aureus in both farm showed mostly multi-drug resistant to several antibiotics which were supported by identification of resistant gene among isolates.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor

et al. 2021

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“…The blaTEM was detected in most isolates, which was positively correlated with the penicillin G resistance phenotype. Previously, studies reported a high frequency of the blaTEM gene in foodborne and clinical isolates (80% and 100%, respectively) (Xu et al 2014;Yang et al 2016). Resistance to methicillin in S. aureus is primarily mediated by the mecA gene, which encodes the low-affinity penicillin-binding protein 2a (PBP2a) (Rolo et al 2017).…”

Section: Antibiotic Resistance Marker Genementioning

confidence: 99%

Multiple antibiotic resistance and virulence factors of Staphylococcus aureus strains isolated from dairy farms in South Sulawesi, Indonesia

et al. 2022

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Abstract. Juwita S, Indrawati A, Damajanti R, Safika, Mayasari NLPI. 2021. Multiple antibiotic resistance and virulence factors of Staphylococcus aureus strains isolated from dairy farms in South Sulawesi, Indonesia. Biodiversitas 23: 1015-1022. Antimicrobial resistance (AMR) is an important issue affecting human and animal health worldwide. This study aimed to investigate antibiotic resistance and determine the virulence factors of S. aureus isolated from the dairy farms in South Sulawesi, Indonesia. Thirty-one isolates of S. aureus were tested for sensitivity to 9 types of antibiotics using the Kirby-Bauer disk diffusion method. The analysis of antibiotic resistance and virulence genes in S. aureus isolates was performed by the conventional PCR method. The results showed that S. aureus isolates from human samples were resistant to penicillin G (PEN) (86%), ampicillin (AMP) (86%), oxacillin (OXA) (14%), cefoxitin (FOX) (14%), tetracycline (TET) (43%) and ciprofloxacin (CIP) (14%). Staphylococcus aureus isolates from the animal samples were resistant to penicillin G (PEN) (50%), ampicillin (AMP) (50%), tetracycline (TET) (15%), and erythromycin (5%). Meanwhile, S. aureus isolates from dangke were resistant to penicillin G (PEN) and ampicillin (AMP) (50% each). Antimicrobial resistance genes for blaTEM (83%), mecA (17%), and tetA (100%) were detected in S. aureus isolates from human samples, whereas those for blaTEM (90%) and tetA (100%) were detected in isolates from animal samples. Meanwhile, the genes for blaTEM (100%) were detected in isolates from dangke. A total of 19 S. aureus isolates harbored the virulence gene for fnbA (26%), clfA (58%), hla (58%), and tst (21%). The use of antibiotics in humans and animals needs to be implemented properly in local communities to prevent the spread of antibiotic resistance. The presence of the tst gene in raw milk is essential for consumer protection against the risk of toxic shock syndrome.

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Prevalence and molecular characterization of plasmidmediated beta-lactamase genes among nosocomial <i>Staphylococcus aureus</i> isolated in Taiwan

Abstract: Purpose: To analyze the drug susceptibility phenotypes and the patterns of plasmid-mediated β-lactamase genes among nosocomial Staphylococcus aureus drug resistance isolates in Taiwan

Cited by 2 publications

References 14 publications

Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor

Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor

Multiple antibiotic resistance and virulence factors of Staphylococcus aureus strains isolated from dairy farms in South Sulawesi, Indonesia

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