Preparation of immobilized glucose oxidase wafer enzyme on calcium-bentonite modified by surfactant

Widi, Restu Kartiko; Trisulo, D C; Budhyantoro, Arief; Chrisnasari, Ruth

doi:10.1088/1757-899x/223/1/012050

Cited by 7 publications

(5 citation statements)

References 13 publications

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“…The results showed that the immobilization of the catalase was slightly better than the immobilization of GOD. In the literature, numerous methods for glucose oxidase immobilization can be found with great differences in their success 3,[8][9][10]12 . Efficiency of immobilization usually depends on the pH, temperature, concentration of enzyme in the solution before immobilization, and the time of immobilization.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

“…Immobilization of GOD provides several benefits that include enhanced stability, easier separation of the product from the reaction medium, increased productivity, and possibility of enzyme reuse. Different methods as well different carriers were tested for GOD immobilization [8][9][10][11][12][13] . Enzyme deactivation caused by aeration can be efficiently avoided by using porous support for immobilization 7,14,15 .…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme

Pečar¹

2019

Chem.Biochem.Eng.Q.

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The effect of aeration on the stability of glucose oxidase in the reaction of glucose oxidation to gluconic acid was investigated by determining the operational stability decay rate constant in the process conditions. Eupergit C as a porous carrier was chosen for the enzyme immobilization. To evaluate glucose oxidase operational stability during process conditions, experiments of glucose oxidation were carried out in the repetitive batch reactor with and without continuous aeration at different aeration levels. It was found that the decay rate of the free enzyme linearly depended on the air flow rate. Immobilization of glucose oxidase on Eupergit C significantly enhanced enzyme stability at higher aeration rates. Kinetics of the free and immobilized enzyme was also determined. The mathematical model of glucose oxidation catalysed by free and immobilized glucose oxidase in the batch reactor was developed.

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Section: Enzyme Immobilizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme

Pečar¹

2019

Chem.Biochem.Eng.Q.

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“…Due to the rapid vaporization, a thin layer of MIP (or NIP) was formed on the sensor's surface. (8) To evaporate the remaining solvent, the sensor was heated at 60 • C for 24 h. ( 9) The final step was to remove the template (glucose) from the MIP by washing the coated senor with DI water for 5 h. In order to deposit MIP and NIP accurately in the desired position (tip of the stub resonator) in sensors, a thin film of Kapton was utilized as a mask, shown in Figure 7a. The microscope image of the uncoated and the MIP-coated biosensor is illustrated in Figure 7b.…”

Section: Preparation Of Proposed Sensormentioning

confidence: 99%

“…Nevertheless, GOD can be damaged by some chemical substances and detergents or changes in its optimal environment. Indeed, those GOD-based glucose sensors are always vulnerable to deactivation due to chemical deformation and possible thermal changes during fabrication, storage, or even while using [8].…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive Molecularly Imprinted Polymer (MIP)-Coated Microwave Glucose Sensor

Omidvar,

Amanati Shahri,

Serrano

et al. 2022

Sensors

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A novel, low-cost, sensitive microwave microfluidic glucose detecting biosensor incorporating molecularly imprinted polymer (MIP) is presented. The sensing device is based on a stub resonator to characterize water glucose solutions. The tip of one of the stubs is coated with MIP to increase the selectivity of the sensor and hence the sensitivity compared to the uncoated or to the coated with non-imprinted polymer (NIP) sensor. The sensor was fabricated on a FR4 substrate for low-cost purposes. In the presence of the MIP, the sensor loaded with a glucose solution ranging from 50 mg/dL to 400 mg/dL is observed to experience an absorption frequency shift of 73 MHz when the solutions flow in a microfluidic channel passing sensing area, while the lower limit of detection (LLD) of the sensor is discovered to be 2.4 ng/dL. The experimental results show a high sensitivity of 1.3 MHz/(mg/dL) in terms of absorption frequency.

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“…In addition, GOD can be damaged by some chemical substances and detergents or changes in its optimal environment for instance, temperatures above 40 °C, pH below 2, or above 8. Indeed, those GOD-based glucose sensors are always vulnerable to deactivation due to chemical deformation and possible thermal changes during fabrication, storage, or even while using (Widi et al, 2017). Because an operational potential was necessary for the amperometric measurement in enzymatic-based sensors, they do not work well with passive sensors.…”

Section: Sensitive Elementsmentioning

confidence: 99%

Projeto, fabricação e aplicação de biossensores de glicose não enzimáticos.

Omidvar¹

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Diabetes is one of the four main noncommunicable diseases and has become one of the fastestgrowing diseases in the world. It will be the seventh leading cause of death worldwide by 2030.The International Diabetes Federation (IDF) has released a new report that demonstrates the global population with diabetes has reached 537 million, increasing 16% since their previous estimation in 2019.Unfortunately, there is no known cure for this chronic health condition; the only alternative to prevent complications for diabetes patients is constantly monitoring their blood glucose levels.Also, the safe range for blood glucose levels is very narrow (between 80-120 mg/dl). Therefore, the sensing method should be able to detect a slight change in concentration in this range. In order to measure the glucose level, biosensors are very practical. A biosensor is transducer capable of converting a biological response into a measurable signal.A novel, low-cost, sensitive microwave microfluidic glucose detecting biosensor incorporating molecularly imprinted polymer is presented. The sensing device is based on dual stub resonators to characterize water glucose solutions. The tip of one of the stubs is coated with MIP to increase the selectivity of the sensor and hence the sensitivity compared to the uncoated or non-imprinted polymer one. The sensor was fabricated on an FR4 substrate for low-cost purposes.In the presence of the MIP, the sensor load-ed with a glucose solution ranging from 50 mg/dL to 400 mg/dL was observed to experience an absorption frequency shift of 73 MHz when the solutions flow in a microfluidic channel passing sensing area, while the Lower Limit of Detection (LLD) of the sensor was discovered to be 2.4 ng/dL. The experimental results showed a high sensitivity of 1.3 MHz /(mg/dL) in terms of absorption frequency.

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Preparation of immobilized glucose oxidase wafer enzyme on calcium-bentonite modified by surfactant

Cited by 7 publications

References 13 publications

Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme

Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme

A Highly Sensitive Molecularly Imprinted Polymer (MIP)-Coated Microwave Glucose Sensor

Projeto, fabricação e aplicação de biossensores de glicose não enzimáticos.

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