Preparation of cell culture scaffolds using polycaprolactone/quince seed mucilage

Allafchian, Alireza; Jalali, Seyed Amir Hossein; Mousavi, Seyed Ebrahim; Hosseini, Seyed Sajjad

doi:10.1016/j.ijbiomac.2019.11.096

Cited by 27 publications

(12 citation statements)

References 45 publications

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“…The higher hydrophilicity of the resultant scaffold is thought to be due to hydrophilic groups, such as hydroxyl and carboxyl groups, which are present in the CMF structure as identified by the GC-MS test. These findings are in agreement with the result of Allafchian et al, who reported that the contact angle for PCL is 120°, while after the addition of quince seed mucilage, the contact angle value of PCL decreased to about 40° [ 41 ].…”

Section: Resultssupporting

confidence: 92%

Electrospun Polycaprolactone/Chitosan Nanofibers Containing Cordia myxa Fruit Extract as Potential Biocompatible Antibacterial Wound Dressings

et al. 2023

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The goal of the current work was to create an antibacterial agent by using polycaprolactone/chitosan (PCL/CH) nanofibers loaded with Cordia myxa fruit extract (CMFE) as an antimicrobial agent for wound dressing. Several characteristics, including morphological, physicomechanical, and mechanical characteristics, surface wettability, antibacterial activity, cell viability, and in vitro drug release, were investigated. The inclusion of CMFE in PCL/CH led to increased swelling capability and maximum weight loss. The SEM images of the PCL/CH/CMFE mat showed a uniform topology free of beads and an average fiber diameter of 195.378 nm. Excellent antimicrobial activity was shown towards Escherichia coli (31.34 ± 0.42 mm), Salmonella enterica (30.27 ± 0.57 mm), Staphylococcus aureus (21.31 ± 0.17 mm), Bacillus subtilis (27.53 ± 1.53 mm), and Pseudomonas aeruginosa (22.17 ± 0.12 mm) based on the inhibition zone assay. The sample containing 5 wt% CMFE had a lower water contact angle (47 ± 3.7°), high porosity, and high swelling compared to the neat mat. The release of the 5% CMFE-loaded mat was proven to be based on anomalous non-Fickian diffusion using the Korsmeyer–Peppas model. Compared to the pure PCL membrane, the PCL-CH/CMFE membrane exhibited suitable cytocompatibility on L929 cells. In conclusion, the fabricated antimicrobial nanofibrous films demonstrated high bioavailability, with suitable properties that can be used in wound dressings.

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Section: Resultssupporting

confidence: 92%

Electrospun Polycaprolactone/Chitosan Nanofibers Containing Cordia myxa Fruit Extract as Potential Biocompatible Antibacterial Wound Dressings

et al. 2023

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“…After the settling of hAMSCs into the pores of cQSM-dS, it was observed that the fibroblastic morphology was turned into the spheroid-like form, due to the non-fouling 3D structure of the cQSM-dS (Figure 3(c)). When compared the viability of the cQSM-dS to the other studies; including Aghmiuni and colleagues, who cultured the human eyelid adipose-derived mesenchymal stem cells on the PCL-QSM-PEG (PQSP) 26 ; and Allafchian et al, 27 that used the Vero cells on the electrospun PCL/QSM scaffold at the ratio of 60:40 (v/v), the MTT results indicated that the absorbance levels and proliferation pattern were akin to each other, and the viability was considerably similar. In this study, the cQSM-dS proved its function as a biocompatible backbone to the cells, and the cell viability was increased day by day without any synthetic polymer additive.…”

Section: Cell Propagation and Determination Of Cell Viability On Cqsm-dsmentioning

confidence: 99%

“…Although the various benefits of QSM, only a little attention was concerned about QSM in the regenerative medicine realm, and best of our knowledge, all of the studies with QSM include synthetic polymer additives. 26,27 In our pioneering study, we aimed at producing a novel and highly porous three-dimensional (3D) physical constructs from the natural biopolymer QSM for hAMSCs to prove the QSM material itself could be an ideal candidate for regenerative approaches. Due to being a green and renewable biomaterial, the reviews about the QSM extraction methods have also drawn attention in the field to increase the yield to meet the commercial demands.…”

Section: Introductionmentioning

confidence: 99%

Bioengineered three-dimensional physical constructs from quince seed mucilage for human adipose-derived mesenchymal stem cells

Şimşek

Karaca

Arslan

2020

Journal of Bioactive and Compatible Polymers

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In this study, we aimed at fabricating a novel porous physical construct from quince seed mucilage for translational medicine applications. To achieve this goal, quince seed mucilage was extracted, molded, and freeze-dried. After being freeze-dried, the molded constructs were chemically crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide to maintain the mechanical integrity of the structure. The fabricated scaffolds were characterized in-depth by scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller analysis, thermogravimetric analysis, and dynamic mechanical analysis in addition to the swelling, liquid uptake, and porosity tests. The extraction yield of mucilage was calculated to be 6.28% ± 0.40% (n = 3). The swelling ratio of crosslinked quince seed mucilage–derived scaffolds was found to be 12,677.50% ± 388.82% (n = 3), whereas the porosity of crosslinked quince seed mucilage–derived scaffolds was 83.43% ± 2.84% (n = 3). The analyses confirmed the crosslinked quince seed mucilage–derived scaffolds to be possessed interconnected, highly porous structure. Afterward, human adipose-derived mesenchymal stem cells were seeded on the crosslinked quince seed mucilage–derived scaffolds, and the cell viability on the scaffolds was assessed with 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The MTT results revealed the scaffolds not to be possessed any cytotoxic effect on seeded cells. Human adipose-derived mesenchymal stem cells adhesion and migration on the crosslinked quince seed mucilage–derived scaffolds were also evaluated histologically using hematoxylin and eosin staining in addition to scanning electron microscopy analysis. In conclusion, we believe that crosslinked quince seed mucilage–derived scaffolds have the potential to be an alternative to routinely used polysaccharides in regenerative medicine applications.

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“…Cell culture technology is the key technology in biomedical research, tissue engineering, regenerative medicine, and biosensing techniques [4][5][6][7]. Cell cultures are helpful for revealing the basic biophysical and molecular mechanisms, which is the key technology for research on cell traits and the development of cell products [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The Application of Polycaprolactone Scaffolds with Poly(ε-caprolactone)–Poly(ethylene glycol)–Poly(ε-caprolactone) Loaded on Kidney Cell Culture

et al. 2022

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Human embryonic kidney cells are the host of adenovirus type-5 (Ad5) amplification. An Ad5-vector-based COVID-19 vaccine has been proven to be tolerated and immunogenic in healthy adults. Therefore, a rationally designed scaffold for culturing human embryonic kidney cells is useful for further studying its mechanism of action. Herein, a three-dimensional layered reticulated polycaprolactone (PCL) scaffold coated with poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC) was developed to proliferate human embryonic kidney cells and to be used to amplify the Ad5 vector. The results indicate that PCEC improves the hydrophilicity and the cell culture ability of PCL cell culture scaffolds, resulting in a three times higher cell proliferation ratio of human embryonic kidney cells compared with those grown on bare PCL cell culture scaffolds. Meanwhile, the cytotoxicity test results showed that the scaffold material is noncytotoxic. This work provides an effective and scalable method for the in-depth study of adenoviruses.

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Preparation of cell culture scaffolds using polycaprolactone/quince seed mucilage

Cited by 27 publications

References 45 publications

Electrospun Polycaprolactone/Chitosan Nanofibers Containing Cordia myxa Fruit Extract as Potential Biocompatible Antibacterial Wound Dressings

Electrospun Polycaprolactone/Chitosan Nanofibers Containing Cordia myxa Fruit Extract as Potential Biocompatible Antibacterial Wound Dressings

Bioengineered three-dimensional physical constructs from quince seed mucilage for human adipose-derived mesenchymal stem cells

The Application of Polycaprolactone Scaffolds with Poly(ε-caprolactone)–Poly(ethylene glycol)–Poly(ε-caprolactone) Loaded on Kidney Cell Culture

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