Preparation of anti‐danofloxacin antibody and development of an indirect competitive enzyme‐linked immunosorbent assay for detection of danofloxacin residue in chicken liver

Liu, Zhongqiu; Lu, Shengxin; Zhao, Cui‐Hua; Ding, Kai; Cao, Zhaozhen; Zhan, Jinhua; Ma, Chen; Liu, Jinting; Xi, Rimo

doi:10.1002/jsfa.3514

Cited by 24 publications

(10 citation statements)

References 21 publications

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“…The protocol used for the indirect ELISA was similar to that described in a previous study . Briefly, coating antigen of CLOP‐2C‐OVA was appropriately diluted in CBS and 100 µL aliquots were added to each well of flat‐bottom polystyrene ELISA plates.…”

Section: Methodsmentioning

confidence: 99%

Development of an enzyme-linked immunosorbent assay for detection of clopidol residues in chicken tissues

Jiang

Zhang

et al. 2014

J. Sci. Food Agric.

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Section: Methodsmentioning

confidence: 99%

Development of an enzyme-linked immunosorbent assay for detection of clopidol residues in chicken tissues

Jiang

Zhang

et al. 2014

J. Sci. Food Agric.

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“…In this study, the immunogen was synthesized by the linkage of carboxylic acid group of ENR with the amino group of the carrier protein (BSA). In this linkage, it is deduced that the ethyl group located at the 4 ′ position of the piperazinyl ring is the furthest group from the linking point, and plays an important role in epitope determination (Liu et al, 2009;Sheng et al, 2009). Of the fluoroquinolones shown in Figure 1, only ENR has an ethyl group linked at the 4 ′ position of the piperazinyl moiety through a nitrogen atom, which may explain the low CR of the obtained anti-ENR antibody toward other fluoroquinolones.…”

Section: Generation and Characterization Of Pabmentioning

confidence: 98%

Development and application of an immunoaffinity column clean-up for enrofloxacin determination in food samples

Wang

Yang

Niu

et al. 2016

Food and Agricultural Immunology

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2017) Development and application of an immunoaffinity column clean-up for enrofloxacin determination in food samples, Food and Agricultural Immunology, 28:2, 248-259, ABSTRACT Enrofloxacin (ENR) is a synthetic fluoroquinolone antibiotic used for the treatment of infection in animals. The use of ENR has been strictly regulated because of its harmful effect on consumers of animal products. In this study the generation of polyclonal antibody (pAb) against ENR, the preparation of an immunoaffinity chromatography column and its potential application to the selective extraction of ENR residues from food samples were described. The produced pAb exhibited good sensitivity to ENR with an IC 50 value of 5.4 ng/mL. The cross-reactivity values of the antibody with ENR structurally related compounds were lower than 0.5%. An immunoaffinity column was constructed by coupling antibody covalently with chitosan microspheres. 0.8% NaOH/0.01 mol/L phosphate-buffered saline (PBS) (pH 7.4) and 85% methanol were selected as loading and eluting solution by careful optimization. Further characterization indicated that the binding capacity of the column for ENR was approximately 3780 ng/mL gel. The immunoaffinity columns were then challenged with ENR-fortified pork, and the recoveries of ENR were found to be in the range of 79.8-94.9%, demonstrating the feasibility of the prepared immunoaffinity column for sample clean-up in ENR residue determination. ARTICLE HISTORY

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“…The structures of fluoro quinolone molecules bear a carboxyl group in a certain fragment; it is most often for the preparation of immu noreagents with the aim to develop a selective method for the determination of quinolones or fluoroquinolo nes. One of the most popular methods of synthesis via the carboxyl group is its activation by the carbodiimide method [5][6][7][8] followed by the addition of a compound with an amino group. It was shown experimentally that the optimum results were obtained with tracers based on fluoroquinolone garenoxacin.…”

Section: Synthesis Of Tracersmentioning

confidence: 99%

Determination of fluoroquinolone antibiotic levofloxacin in urine by fluorescence polarization immunoassay

et al. 2015

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A method of fluorescence polarization immunoassay (FPIA) has been developed for the determi nation of levofloxacin in urine. Fluorescein tagged antigens (tracers) with various fluoroquinolones and flu orescent tags have been synthesized. The tracer based on garenoxacin labeled with 4 aminomethylfluorescein and polyclonal antibodies to levofloxacin was the most sensitive for the determination of fluoroquinolones. A FPIA method has been developed for the determination of levofloxacin with the limit of detection 1.0 ng/mL and analytical range from 2.5 to 50 ng/mL. A one reagent FPIA method has been developed for the determi nation of levofloxacin with the limit of detection 0.5 ng/mL and analytical range from 1 to 10 ng/mL. The methods have been tested in the determination of levofloxacin in urine.

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Preparation of anti‐danofloxacin antibody and development of an indirect competitive enzyme‐linked immunosorbent assay for detection of danofloxacin residue in chicken liver

Cited by 24 publications

References 21 publications

Development of an enzyme-linked immunosorbent assay for detection of clopidol residues in chicken tissues

Development of an enzyme-linked immunosorbent assay for detection of clopidol residues in chicken tissues

Development and application of an immunoaffinity column clean-up for enrofloxacin determination in food samples

Determination of fluoroquinolone antibiotic levofloxacin in urine by fluorescence polarization immunoassay

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