Development and application of an immunoaffinity column clean-up for enrofloxacin determination in food samples

Abstract

2017) Development and application of an immunoaffinity column clean-up for enrofloxacin determination in food samples, Food and Agricultural Immunology, 28:2, 248-259, ABSTRACT Enrofloxacin (ENR) is a synthetic fluoroquinolone antibiotic used for the treatment of infection in animals. The use of ENR has been strictly regulated because of its harmful effect on consumers of animal products. In this study the generation of polyclonal antibody (pAb) against ENR, the preparation of an immunoaffinity chromatography column and its potential application to the selective extraction of ENR residues from food samples were described. The produced pAb exhibited good sensitivity to ENR with an IC 50 value of 5.4 ng/mL. The cross-reactivity values of the antibody with ENR structurally related compounds were lower than 0.5%. An immunoaffinity column was constructed by coupling antibody covalently with chitosan microspheres. 0.8% NaOH/0.01 mol/L phosphate-buffered saline (PBS) (pH 7.4) and 85% methanol were selected as loading and eluting solution by careful optimization. Further characterization indicated that the binding capacity of the column for ENR was approximately 3780 ng/mL gel. The immunoaffinity columns were then challenged with ENR-fortified pork, and the recoveries of ENR were found to be in the range of 79.8-94.9%, demonstrating the feasibility of the prepared immunoaffinity column for sample clean-up in ENR residue determination. ARTICLE HISTORY

Recovery of the Structure and Function of the Pig Manure Bacterial Community after Enrofloxacin Exposure

“…This effect of enrofloxacin on the intestinal bacterial community may not be long-lasting. However, previous studies have shown that enrofloxacin residues in edible animal products may have adverse effects on humans, such as inducing allergic allergies, reducing sperm count, and diminishing sperm motility ( 54 , 55 ). Therefore, although enrofloxacin exposure did not cause permanent changes in the manure bacterial community, attention should be given to its effects on other organs.…”

Development and Validation of a Reproducible and Label-Free Surface Plasmon Resonance Immunosensor for Enrofloxacin Detection in Animal-Derived Foods

“…To date, instrumental analysis based on chromatographic separation techniques [ 13 , 14 ] and immunoassays [ 15 , 16 ] based on antigen (Ag)-antibody (Ab) binding are the major analytical methods for ENRO measurement in food samples. In our previous studies, we prepared a molecularly imprinted biomimetic material [ 17 ] and polyclonal Ab with specific ability to recognize ENRO and developed relative instrumental and immunoassays/products for ENRO residue detection in food products.…”