Recovery of the Structure and Function of the Pig Manure Bacterial Community after Enrofloxacin Exposure

Chen, Tao; Xie, Gaomiao; Mi, Jiandui; Wen, Xin; Cao, Zhen; Ma, Baohua; Zou, Yongde; Zhang, Na; Wang, Yan; Liao, Xindi; Wu, Yinbao

doi:10.1128/spectrum.02004-21

Cited by 10 publications

(5 citation statements)

References 59 publications

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“…The species Terrisporobacter petrolearius JCM 19845 T , isolated in 2015 from a petroleum reservoir sample from an oilfield in Shengli (China) ( Deng et al, 2015 ), and Terrisporobacter hibernicus NCTC 14625 T , isolated in 2023 from bovine feces, were both described as non-acetogenic species ( Mitchell et al, 2023 ). The genus Terrisporobacter has been found strongly associated with manure ( Chen et al, 2022 ) and was shown to represent a key player in the degradation of manure by fermenting carbohydrates to H 2 + CO 2 and acetate ( Budianto and Yasin, 2024 ). Correspondingly, biotechnological applications of bacteria from the genus Terrisporobacter have focused on their H 2 -producing capabilities.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Böer,

Schüler,

Lüschen

et al. 2024

Front. Microbiol.

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Due to their metabolic versatility in substrate utilization, acetogenic bacteria represent industrially significant production platforms for biotechnological applications such as syngas fermentation, microbial electrosynthesis or transformation of one-carbon substrates. However, acetogenic strains from the genera Terrisporobacter and Acetoanaerobium remained poorly investigated for biotechnological applications. We report the isolation and characterization of four acetogenic Terrisporobacter strains and one Acetoanaerobium strain. All Terrisporobacter isolates showed a characteristic growth pattern under a H2 + CO2 atmosphere. An initial heterotrophic growth phase was followed by a stationary growth phase, where continuous acetate production was indicative of H2-dependent acetogenesis. One of the novel Terrisporobacter isolates obtained from compost (strain COMT) additionally produced ethanol besides acetate in the stationary growth phase in H2-supplemented cultures. Genomic and physiological characterizations showed that strain COMT represented a novel Terrisporobacter species and the name Terrisporobacter vanillatitrophus is proposed (=DSM 116160T = CCOS 2104T). Phylogenomic analysis of the novel isolates and reference strains implied the reclassification of the T. petrolearius/T. hibernicus phylogenomic cluster to the species T. petrolearius and of the A. noterae/A. sticklandii phylogenomic cluster to the species A. sticklandii. Furthermore, we provide first insights into active prophages of acetogens from the genera Terrisporobacter and Acetoanaerobium.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Böer,

Schüler,

Lüschen

et al. 2024

Front. Microbiol.

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“…The susceptibility of bacteria to enrofloxacin is important at both the public health and pig health levels, as it is still commonly used in most countries, despite its public health importance being as high as that of colistin [ 14 , 35 , 36 , 37 , 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Actinobacillus pleuropneumoniae, Pasteurella multocida and Streptococcus suis Isolated from Pigs in Hungary between 2018 and 2021

et al. 2023

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Porcine respiratory disease complex (PRDC) has been a major animal health, welfare, and economic problem in Hungary; therefore, great emphasis should be put on both the prevention and control of this complex disease. As antibacterial agents are effective tools for control, antibiotic susceptibility testing is indispensable for the proper implementation of antibacterial therapy and to prevent the spread of resistance. The best method for this is to determine the minimum inhibitory concentration (MIC) by the broth microdilution method. In our study, we measured the MIC values of 164 Actinobacillus pleuropneumoniae, 65 Pasteurella multocida, and 118 Streptococcus suis isolates isolated from clinical cases against the following antibacterial agents: amoxicillin, ceftiofur, cefquinome, oxytetracycline, doxycycline, tylosin, tilmicosin, tylvalosin, tulathromycin, lincomycin, tiamulin, florfenicol, colistin, enrofloxacin, and sulfamethoxazole-trimethoprim. Outstanding efficacy against A. pleuropneumoniae isolates was observed with ceftiofur (100%) and tulathromycin (100%), while high levels of resistance were observed against cefquinome (92.7%) and sulfamethoxazole-trimethoprim (90.8%). Ceftiofur (98.4%), enrofloxacin (100%), florfenicol (100%), and tulathromycin (100%) were found to be highly effective against P. multocida isolates, while 100% resistance was detected against the sulfamethoxazole-trimethoprim combination. For the S. suis isolates, only ceftiofur (100%) was not found to be resistant, while the highest rate of resistance was observed against the sulfamethoxazole-trimethoprim combination (94.3%). An increasing number of studies report multi-resistant strains of all three pathogens, making their monitoring a high priority for animal and public health.

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“…Under enrofloxacin stress, the structure of the fecal bacterial community in pigs changes, with a significant increase in the abundance of Firmicutes . Furthermore, enrofloxacin-induced differences in the intestinal flora were found to be fully recovered 10 days after the discontinuation of the drug ( 21 ). Moreover, corticosterone can affect carbohydrate metabolism and amino acid metabolism in chicks by altering gut microbes ( 22 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of Enrofloxacin on the Microbiome, Metabolome, and Abundance of Antibiotic Resistance Genes in the Chicken Cecum

Mei

et al. 2023

Microbiol Spectr

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Recovery of the Structure and Function of the Pig Manure Bacterial Community after Enrofloxacin Exposure

Abstract: A stable intestinal bacterial community balance is beneficial for animal health. Enrofloxacin is widely used in animal husbandry as a therapeutic drug, but it can cause intestinal environmental imbalance.

Cited by 10 publications

References 59 publications

Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Isolation and characterization of novel acetogenic strains of the genera Terrisporobacter and Acetoanaerobium

Susceptibility of Actinobacillus pleuropneumoniae, Pasteurella multocida and Streptococcus suis Isolated from Pigs in Hungary between 2018 and 2021

Effect of Enrofloxacin on the Microbiome, Metabolome, and Abundance of Antibiotic Resistance Genes in the Chicken Cecum

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