Determination of fluoroquinolone antibiotic levofloxacin in urine by fluorescence polarization immunoassay

Shanin, I. A.; Shaimardanov, A. R.; Thai, Nguen Ti Diu; Eremin, Sergei A.

doi:10.1134/s1061934815060167

Cited by 10 publications

(3 citation statements)

References 8 publications

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“…The observed limit of detection was 5 μg kg −1 (Beloglazova, Shmelin, & Eremin, 2016). Shanin, Shaimardanov, Thai, and Eremin (2015) developed one reagent FPIA for the screening of levofloxacin in urine samples (Shanin et al, 2015). Another novel approach for multiplexed FPIA has been established for the determination of SA and FQ which is based on bi-specific single-chain diabody (Chen et al, 2014).…”

Section: Fluorescence Immunoassay/fluorescence Polarization Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

124

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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Section: Fluorescence Immunoassay/fluorescence Polarization Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

124

View full text Add to dashboard Cite

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“…The fluorescence polarization immunoassay (FPIA) assay of levofloxacin in urine was also described by Shanin et al [44]. The achieved LOD value was 0.5 ng/mL [44].…”

Section: Immunoassaysmentioning

confidence: 99%

Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples

2020

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Antibiotics were initially natural substances. However, nowadays, they also include synthetic drugs, which show their activity against bacteria, killing or inhibiting their growth and division. Thanks to these properties, many antibiotics have quickly found practical application in the fight against infectious diseases such as tuberculosis, syphilis, gastrointestinal infections, pneumonia, bronchitis, meningitis and septicemia. Antibiotic resistance is currently a detrimental problem; therefore, in addition to the improvement of antibiotic therapy, attention should also be paid to active metabolites in the body, which may play an important role in exacerbating the existing problem. Taking into account the clinical, cognitive and diagnostic purposes of drug monitoring, it is important to select an appropriate analytical method that meets all the requirements. The detection and identification of the microorganism responsible for the infection is also an essential factor in the implementation of appropriate antibiotic therapy. In recent years, clinical microbiology laboratories have experienced revolutionary changes in the way microorganisms are identified. The MALDI-TOF MS technique may be interesting, especially in some areas where a quick analysis is required, as is the case with clinical microbiology. This method is not targeted, which means that no prior knowledge of the infectious agent is required, since identification is based on a database match.

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“…These techniques include enzyme-linked immunosorbent assays (ELISA), which are able to detect gentamicin and vancomycin in samples containing proteins with ranges of 2–500 ng/mL for gentamicin and 20–5000 ng/mL for vancomycin [ 236 ]. Fluoroquinolones, such as levofloxacin, have also been detected in urine using a fluorescence polarization immunoassay assay ranging from 2.5 to 50 ng/mL based on garenoxacin labeled with 4-aminomethylfluorescein and polyclonal antibodies (pAb) against levofloxacin [ 237 ] ( Table 3 ). For an AB like colistin, pAb have been used for quantification, mainly in food [ 238 , 239 ].…”

Section: Therapeutic Drug Monitoring (Tdm)mentioning

confidence: 99%

Personalized Medicine for Antibiotics: The Role of Nanobiosensors in Therapeutic Drug Monitoring

Garzón

Bustos

Pinacho

2020

JPM

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Due to the high bacterial resistance to antibiotics (AB), it has become necessary to adjust the dose aimed at personalized medicine by means of therapeutic drug monitoring (TDM). TDM is a fundamental tool for measuring the concentration of drugs that have a limited or highly toxic dose in different body fluids, such as blood, plasma, serum, and urine, among others. Using different techniques that allow for the pharmacokinetic (PK) and pharmacodynamic (PD) analysis of the drug, TDM can reduce the risks inherent in treatment. Among these techniques, nanotechnology focused on biosensors, which are relevant due to their versatility, sensitivity, specificity, and low cost. They provide results in real time, using an element for biological recognition coupled to a signal transducer. This review describes recent advances in the quantification of AB using biosensors with a focus on TDM as a fundamental aspect of personalized medicine.

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Determination of fluoroquinolone antibiotic levofloxacin in urine by fluorescence polarization immunoassay

Cited by 10 publications

References 8 publications

Current advances in immunoassays for the detection of antibiotics residues: a review

Current advances in immunoassays for the detection of antibiotics residues: a review

Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples

Personalized Medicine for Antibiotics: The Role of Nanobiosensors in Therapeutic Drug Monitoring

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