A method of fluorescence polarization immunoassay (FPIA) has been developed for the determi nation of levofloxacin in urine. Fluorescein tagged antigens (tracers) with various fluoroquinolones and flu orescent tags have been synthesized. The tracer based on garenoxacin labeled with 4 aminomethylfluorescein and polyclonal antibodies to levofloxacin was the most sensitive for the determination of fluoroquinolones. A FPIA method has been developed for the determination of levofloxacin with the limit of detection 1.0 ng/mL and analytical range from 2.5 to 50 ng/mL. A one reagent FPIA method has been developed for the determi nation of levofloxacin with the limit of detection 0.5 ng/mL and analytical range from 1 to 10 ng/mL. The methods have been tested in the determination of levofloxacin in urine.
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