Preparation, characterization and in vitro analysis of novel structured nanofibrous scaffolds for bone tissue engineering

Wang, J.; Yu, Xiaojun

doi:10.1016/j.actbio.2010.01.045

Cited by 51 publications

(42 citation statements)

References 23 publications

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“…For instance, PLGA tubular scaffolds with a PCL nanofiber spiral structured inner core were developed that displayed improved mechanical properties. 31 Pure PCL, however, does not improve the biocompatibility when compared with pure poly(d,l-lacticco-glycolic acid), and, therefore, its combined application is limited apart from its use when a delayed degradation of the implant material is desired. 82 PLGA-ceramic scaffolds.…”

Section: Scaffolds Consisting Of Pure Plgamentioning

confidence: 99%

“…Moreover, PLGA is most often used in combination other ceramic or polymeric materials that interact with PLGA, thereby enhancing the difficulty to draw unambiguous conclusions on the specific effects of physicochemical characteristics of PLGA on ultimate performance in bone regeneration. In this regard, it is important to notice that relevant information on, for example, polymer molecular weight, [30][31][32][33][34][35] stereochemistry, [31][32][33][34] or end-group functionalization, [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53] are often missing, which complicates scientific progress even further.…”

Section: Degradationmentioning

confidence: 99%

See 1 more Smart Citation

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Lanao

Jonker

Wolke

et al. 2013

Tissue Engineering Part B: Reviews

167

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Section: Scaffolds Consisting Of Pure Plgamentioning

confidence: 99%

Section: Degradationmentioning

confidence: 99%

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Lanao

Jonker

Wolke

et al. 2013

Tissue Engineering Part B: Reviews

167

View full text Add to dashboard Cite

“…These losses and faults necessitate many researches in the surgical field [14]. In the past decades, in order to amend bone defects, many people opted for organ transplantation techniques such as allograft and autograft [13].…”

mentioning

confidence: 99%

Marine Algae Derived Polysaccharides for Bone Tissue Regeneration

Venkatesan

Kim

2015

Marine Algae Extracts

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“…17 Electrospun fibers are used widely in biomedical applications (as tissue engineering scaffolds or biomimetic substitutes), wound healing, and drug delivery, with further applications being the subject of ongoing research. 18,19 In this study, we used electrospinning followed by biomimetic mineralization to produce fibers with a structure conducive to the proliferation, metabolism, and osteogenic differentiation of human PDLCs. This fibrous scaffold might be innovatively applied in periodontal tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells

Wu¹,

Miao²,

Yao³

et al. 2014

IJN

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Periodontal repair is a complex process in which regeneration of alveolar bone is a vital component. The aim of this study was to develop a biodegradable scaffold with good biocompatibility and osteoinductive ability. Two types of composite fibrous scaffolds were produced by electrospinning, ie, type I collagen/poly(ε-caprolactone) (COL/PCL) and type I collagen/poly(ε-caprolactone)/nanoscale hydroxyapatite (COL/PCL/nHA) with an average fiber diameter of about 377 nm. After a simulated body fluid (SBF) immersion test, the COL/PCL/nHA-SBF scaffold developed a rough surface because of the calcium phosphate deposited on the fibers, suggesting that the presence of nHA promoted the mineralization potential of the scaffold. Energy dispersive X-ray spectroscopy clearly showed the calcium and phosphorus content in the COL/PCL/nHA and COL/PCL/nHA-SBF scaffolds, confirming the findings of nHA and calcium phosphate precipitation on scanning electron micrographs. Water contact analysis revealed that nHA could improve the hydrophilic nature of the COL/PCL/nHA-SBF scaffold. The morphology of periodontal ligament cells cultured on COL/PCL-SBF and COL/PCL/nHA-SBF was evaluated by scanning electron microscopy. The results showed that cells adhered to either type of scaffold and were slightly spindle-shaped in the beginning, then extended gradually with stretched filopodia, indicating an ability to fill the fiber pores. A Cell Counting Kit-8 assay showed that both scaffolds supported cell proliferation. However, real-time quantitative polymerase chain reaction analysis showed that expression of the bone-related markers, alkaline phosphatase and osteocalcin, was upregulated only on the COL/PCL/nHA-SBF scaffold, indicating that this scaffold had the ability to induce osteogenic differentiation of periodontal ligament cells. In this study, COL/PCL/nHA-SBF produced by electrospinning followed by biomimetic mineralization had combined electrospun fibers with nHA in it. This scaffold has good biocompatibility and osteoinductive ability as a result of the characteristics of nHA, so could be innovatively applied to periodontal tissue engineering as a potential scaffold.

show abstract

Preparation, characterization and in vitro analysis of novel structured nanofibrous scaffolds for bone tissue engineering

Cited by 51 publications

References 23 publications

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Physicochemical Properties and Applications of Poly(lactic-co-glycolic acid) for Use in Bone Regeneration

Marine Algae Derived Polysaccharides for Bone Tissue Regeneration

Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells

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