Possible role of autophagy activation in stimulation of regeneration

Vishnyakova, Khava S.; Попов, К. В.; Vorotelyak, E. A.; Файзуллин, Р. Р.; Artyuhov, Alexander; Yegorov, Yegor E.

doi:10.1134/s002689331305021x

Cited by 3 publications

(5 citation statements)

References 41 publications

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“…Such accumulation is typical for keratinocytes [31]. This result was not unexpected, because previously we have shown that RSHG does not stimulate proliferation of various cells in vitro [22].…”

Section: Discussionsupporting

confidence: 71%

“…Obtained results together with earlier ones allow us to propose hypothesis about mechanisms of RSHG action. Previously we demonstrated that RSHG increases cell death under conditions of oxidative stress [22]. Oxidative stress is known to induce cell damage and senescence in vivo.…”

Section: Discussionmentioning

confidence: 89%

“…We suggest that RSHG action is not restricted to the stimulation of senescent cells elimination. Previously we have shown that RSHG induce autophagy [22]. Mild autophagy exhibits anti-aging activity [40].…”

Section: Discussionmentioning

confidence: 95%

“…For many years, the balsam "Satura® Rosta" (RSHG) has been known as an effective remedy for baldness treatment. We began to investigate its action and discovered that RSHG can induce adipogenic differentiation [21] and activate autophagy [22]. A few years ago, we began experiments involving long-term application of RSHG to mice.…”

Section: Introductionmentioning

confidence: 99%

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Hair growth stimulation by a Natural Remedy: Animal Studies

Vishnyakova¹,

Vetkova²,

Jasko³

et al. 2018

Madridge J Dermatol Res

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Hair follicles normally regenerate cyclically and this makes them a popular model for investigations of regeneration and aging. We studied the effects on mouse hair follicles of the Satura ® Rosta remedy manufactured from plant extracts and used to stimulate hair growth restoration. A prolonged application of the remedy to the skin of various strains of mice and of outbred white mice did not induce detectable negative effects and caused a significant increase of mean hair follicle size and hair root volume as well as skin thickening due to adipose elaboration. The volume increase took place mainly among the largest hair follicles. Mouse skin thickness is known to fluctuate cyclically in coordination with the waves of hair growth; anagen regions are thicker due to adipose increase. Satura ® Rosta did not abrogate hair growth waves. Apparently the observed effects could be explained by the remedy-induced prolongation of the anagen phase. Although the effect of Satura ® Rosta on fur appearance was insignificant in young animals, experiments with old mice exhibiting alopecia resulted in hair regrowth in alopecia regions. Transcriptome analysis of Satura ® Rosta action on dermal fibroblasts in vitro revealed significant changes of the expression of genes associated with the cell cycle, developmental processes and lipid metabolism.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Hair growth stimulation by a Natural Remedy: Animal Studies

Vishnyakova¹,

Vetkova²,

Jasko³

et al. 2018

Madridge J Dermatol Res

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show abstract

“…During age, autophagy activity generally declines (Lopez-Otin et al, 2013). Such attenuated autophagy plays an important role in stem cell exhaustion and hair follicle loss (Castilho et al, 2009; Vishniakova Kh et al, 2013). However, the molecular mechanisms that result in the declined autophagy in hair follicles remain unclear.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs-103/107 Regulate Autophagy in the Epidermis

Wang

Kobeissi

Dong

et al. 2018

Journal of Investigative Dermatology

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We have shown that microRNAs-103 and -107 (miRs-103/107) positively regulate end-stage autophagy by ensuring dynamin activity in cultured keratinocytes. Most work in end-stage autophagy has been conducted using in vitro model systems. In vivo regulation of end-stage autophagy in epidermis remains unknown. Here, we used antagomirs to subcutaneously knock down miR-107 in the skin; conversely, we delivered miR-107 mimic subcutaneously via in vivo transfection to increase this miR. We found that antagomir-107 treatment in epidermis: (i) depleted endogenous miR-107; (ii) increased GFP-LC3 puncta in epidermal basal layers of GFP-LC3 transgenic mice, indicative of an accumulation of autophagosomes; (iii) inhibited LC3 turnover and increased p62, suggesting an inhibition of autophagy flux; and (iv) increased phosphorylated dynamin (p-dynamin, an inactive form), a key enzyme in end-stage autophagy. Conversely, miR-107 mimic treatment in mouse epidermis: decreased GFP-LC3 puncta in basal layer, as well as p62 protein levels; and diminished p-dynamin, indicative of activation of this enzyme. In human epidermal keratinocytes, antagos-103/107 cause the formation of large vacuoles and an increase in p-dynamin, which can be rescued by inhibition of protein kinase C pathway. Collectively, these results suggest that the miR-103/107 family has a critical role in regulating end-stage autophagy in mouse epidermis via PLD1/2-protein kinase C-dynamin pathway.

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