Possible protective effect of olive leaves extract on paracetamol induced hepatotoxicity in male albino rats

Abstract

Paracetamol (PCM) overdose can cause hepatotoxicity with oxidative stress; the present study was carried out to establish the possible protective effect of olive leaves extract (OLE) on toxicity induced by paracetamol in adult male rats. Twenty four adult male rats were divided into four equal groups; control, olive leaves extract group, paracetamol group and olive leaves extract plus paracetamol group. Some biochemical parameters and liver histopathology were evaluated. PCM treatment significantly increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), urea, creatinine and alpha-fetoprotein. Paracetamol was found to significantly increase malonaldehyde (MDA) and decrease glutathione reductase (GR) activity in tissue and significantly decrease total antioxidant capacity (TAC) and superoxide dismutase (SOD) in serum. Administration of OLE caused a significant decrease serum AST, ALT enzyme, total bilirubin, GGT, LDH, creatinine, urea, alpha-fetoprotein. Also, amelioration of oxidant – antioxidant status with olive leaves extract was observed in addition to a significant decrease in MDA and a significant increase in TAC in liver tissue with a significant increase in glutathione reductase (GR) and SOD in serum compared to paracetamol treated group The chemical pathological changes were in step with histopathological observation suggesting marked hepatoprotective result of olive leaves extract. It could be concluded that olive leaves extract (OLE) treatment may be effective in decreasing hepatic injury and oxidative stress induced by paracetamol overdose in male albino rats.

Hepatoprotective and Reno-protective Effects of Artichoke Leaf Extract and Rosemary Extract against Paracetamol Induced Toxicity in Albino Rats

“…Our findings agree with El Sayed et al, 2020 who reported that PCM increased hepatic liver enzymes, total bilirubin, (GGT) and (LDH) [37].…”

Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl4-Induced Liver Damage

“…Oleuropein and hydroxytyrosol, as major compounds of olive leaves, have been reported to exert numerous pharmacological properties, including anticancer, antidiabetic, and anti-inflammatory activities [ 14 ]. Recently, the hepatoprotective activity of olive leaves against damage induced by CCl 4 [ 24 , 25 ], cadmium [ 26 , 27 ], paracetamol [ 28 ], thioacetamide [ 29 ], ethanol [ 5 ], fluoxetine [ 30 ] and deltamethrin [ 31 ] has receieved increasing interest in animal experiemts. However, available data about the hepatoprotective effect of olive leaf extracts or their major constituents are still scarce.…”

Assessment of antioxidant traits and protective action of Egyptian acacia pods extracts against paracetamol‐induced liver toxicity in rats

“…Administration of hydrolyzed EtOAC fraction at pH 4 of brown pods extract at 100 and 200 mg/kg showed the capability to reduce liver enzyme levels such as alanine aminotransferase enzymes, ALP and bilirubin to its basal levels. Such effect might be attributed to the antioxidant capacity of these extracts, which would be related to having phenolic and flavonoid compounds (Afsar, Razak, & Almajwal, 2019; Taha, Kamal, & Ibrahim, 2019).…”