Assessment of antioxidant traits and protective action of Egyptian acacia pods extracts against paracetamol‐induced liver toxicity in rats

Abstract

This study investigates the protective effect of Egyptian acacia pod extracts against overdose of paracetamol‐induced liver damage. Egyptian acacia green and brown pods were extracted by mixture of ethanol 80%: HCl (6 M) (99:1 v/v). In extracts of green and brown pods, total phenolic content in hydrolyzed ethyl acetate fraction (HEF) at pH 4, was 649.89 and 712.14 mg GAE/g while antioxidant activity was 95.55% and 97.35%, both being the highest than any fraction. HEF (pH 4) in brown pods was analyzed by HPLC, there were 22 phenolic compounds rich in ethyl vanillin about 227 mg/g and 11 flavonoids rich in catechin 48.70 mg/g. A biological experiment was conducted using HEF (pH4) in brown pods against overdose of paracetamol in albino rats induced to hepatotoxicity. Thirty rats were divided into five groups; a control group, a paracetamol group, and the other three received paracetamol plus silymarin or two doses of HEF. Animals were received paracetamol and treated with either silymarin or HEF showed reduced levels of liver (ALT, AST, and ALP) and kidney (urea, creatinine, and uric acid) markers compared with the control group as well as reduction of oxidative stress and increment antioxidant enzyme activity in liver tissue when compared with the paracetamol group. It could be concluded that both HEF and silymarin are considerably high hepatoprotector against paracetamol‐induced hepatotoxicity in rats due to their strong antioxidant activity. Practical applications Both HEF and silymarin improved liver functions and exerted strong antioxidant activities. This antioxidant activity would have a positive effect against oxidative liver damage caused by parcetamol. Thus, it may be concluded that the liver plasma membranes were protected and the regenerative and reparative capacity of liver by phenolic compound in HEF treatment. The study demonstrated the HEF hepatoprotective activity and recommends using Egyptian acacia pods for treatment of liver disorders.

Safely effective hypoglycemic action of stevia and turmeric extracts on diabetic Albino rats

“…This fact may also explain the higher hepatic protective effect of turmeric than STE, as having higher antioxidant capacity (Table 4). The association between the antioxidant action and the hepatic protective effect is well established (Osman et al., 2019); (Abdel‐Hamid et al.., 2020) and (Salman et al., 2020). It was also reported that curcumin not only induces the downregulation of oxidative stress but also reduces matrix metalloproteinases in liver injury.…”

Standardization of reference values of hepatic and renal biochemical markers in rats (Rattus novergicus) Wistar lineage

“…Assim como as proteínas totais, dificilmente ocorre uma pesquisa isolada e interpretação de resultados apenas com esse parâmetro. Outro ponto em comum, SASIDHARAN et al, 2010). Embora seja um parâmetro importante e um bom marcador para avaliar o funcionamento do fígado, ela não é muito eficaz na determinação da extensão da lesão hepatocelular (SASIDHARAN et al, 2010).…”

A simple conversion of expired medicines into nontoxic activated carbon for energy storage applications

“…While acetaminophen with caffeine anhydrous slightly enhances the activity but is not considered as a good antibacterial agent. 1 Al-Janabi and Ali Abdul Hussein, 2010, state insignificant biological activity of acetaminophen Paracoccus yeei and S aureus. 54 In support of this, our study found that 100 mg/mL of acetaminophen produced no significant antimicrobial activity as compared to positive controls like nitrofurazone (100 μg/disc) and fluconazole (10 μg/disc).…”

“…Recent advancement in the pharmacology is that there are plenty of formulations of acetaminophen that have been used either alone or in combination with other medications. 1 Owing to increased population, dramatic changes in the climatic condition, unhealthy habits and life style and increasing consumption of medicines is observed globally. 2 However, many reports mentioned that large number of medicines remain unused or expired, due to the poor adherence of patients to medication, unwanted and excessive prescribing of medicines by the physicians, and unsuitable storage condition of the medicines.…”

“…Similarly, fast release formulation of acetaminophen was inefficient against pathogenic bacteria like S aureus and E coli at the concentration of 600 mg/mL. While acetaminophen with caffeine anhydrous slightly enhances the activity but is not considered as a good antibacterial agent 1. Al-Janabi and Ali Abdul Hussein, 2010, state insignificant biological activity of acetaminophen Paracoccus yeei and S aureus 54.…”