Positive effects of a <i>Clostridium butyricum</i>-based compound probiotic on growth performance, immune responses, intestinal morphology, hypothalamic neurotransmitters, and colonic microbiota in weaned piglets

Cao, Guangtian; Tao, Fei; Hu, Yuhua; Li, Zhanming; Zhang, Yan; Deng, Bo; Zhan, Xiuan

doi:10.1039/c8fo02370k

Cited by 77 publications

(84 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ileum is highly susceptible to gut pathological events which may concern weaning piglets, and therefore its possible structural and functional changes linked to nutraceutical administration are of a predictive value in the view of judging intestinal defensive responsiveness (Domeneghini et al 2004). Some published papers targeted the ileum to evaluate the effect of feed additives with presumed beneficial effect on performance and inflammatoryand immune-related parameters (Chen et al 2018;Li et al 2018;Cao et al 2019) In addition, in our study we were expecting the major effects in gene expression occurring in this tract of the intestine according to the results reported by Schokker et al (2015), who outlined as in 55-day-old piglets only the ileum displayed differences in immune-related processes when compared to the jejunum. The authors attributed this result to the fact that, in differentiated and matured ileum, immunological structures like Peyer's patches are much more abundant as compared to jejunum.…”

Section: Inflammatory and Immune Responsementioning

confidence: 87%

Effects of nucleotides administration on growth performance and immune response of post-weaning piglets

Perricone

Comi

Bontempo

et al. 2020

Italian Journal of Animal Science

View full text Add to dashboard Cite

The aim of this study was to assess the effect of nucleotides administration on growth performance and immune response in post-weaning piglets. Twenty-eight male weaned piglets, homogeneous for age and weight were randomly allocated to two experimental treatments. Treated group (T) was daily orally administered 0.8 g/head of a mixture of nucleotides suspended in 2.1 mL water solution; while control group (C) received 2.1 mL saline solution. Body weight (BW) and average daily gain (ADG) were individually recorded weekly, while feed intake (FI), and gain:feed (G:F) were recorded and calculated on pen basis. Faecal score was evaluated every seven days. On day 0, 9, 18 and 27 blood samples were collected to determine IgA, IgG and haptoglobin concentration. At day 28 all piglets were sacrificed, and tissue samples of ileal Peyer's patches were collected for the evaluation of IL1a, IL1b, IL6, IL10, TNFa, TLR2, TLR4 and PPARc gene expression. Nucleotides supplementation significantly increased BW (17.37 vs. 19.00 kg/pig; p ¼ <.01), ADG (.351 vs. .400 kg/d; p < .01), and FI (3.96 vs. 4.39 kg/d; p < .01), but not G:F (.61 vs. .64; p ¼ .29). Faecal consistency was not different between the experimental groups and no occurrence of diarrhoea was reported. IgA and IgG content in blood was not influenced by the treatment, as well as gene expression of inflammatory cytokines in Peyer's patches. The present trial shows that nucleotide administration is able to improve growth performance of post-weaning piglets, with no effects on inflammatory response and the expression of immune-related genes. HIGHLIGHTS Nucleotides administration increased BW, ADG and FI. Nucleotides did not affect inflammatory and immune response.

show abstract

Section: Inflammatory and Immune Responsementioning

confidence: 87%

Effects of nucleotides administration on growth performance and immune response of post-weaning piglets

Perricone

Comi

Bontempo

et al. 2020

Italian Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…A total of 30 mice ( n = 6 in each group) were selected to analyse the colonic microflora as per our previous study (Cao et al , ). DNA from the colonic contents was isolated using the MoBio Power Soil DNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA) according to the manufacturer’s manual.…”

Section: Methodsmentioning

confidence: 99%

Bacillus licheniformis , a potential probiotic, inhibits obesity by modulating colonic microflora in C57BL/6J mice model

et al. 2019

Self Cite

View full text Add to dashboard Cite

Aims: This study evaluated the effects of a potential probiotic, Bacillus sp., on the growth, serum and hepatic triglyceride, histological features of liver tissues and colonic microflora in high-fat diet-induced obese mice. Methods and Results: Sixty male C57BL/6J mice were randomly divided into five groups: mice fed a low-fat diet (Cont), mice fed a high-fat diet (Hf), Hf and orally challenged with Bacillus subtilis (Bs), B. licheniformis (Bl) and a mixture of B. subtilis and B. licheniformis (Bls). Gavage feeding was provided at week 9 and the experiment was continued for 8 weeks. Treatment with B. licheniformis and a mixture of Bacillus sp. attenuated body weight gain at the end of study and enhanced glucose tolerance by sensitizing insulin action in the Hf-fed mice. Lower serum and hepatic triglyceride and epididymal fat weight were observed in Bl and Bls groups than that of Hf group. Lesser hepatic fat deposition was observed in the Bl and Bls groups than in the Hf group. High-throughput sequencing showed that Bacillus sp. supplementation dramatically changed the colonic bacterial community in obese mice. Conclusions: Bacillus licheniformis reduced body weight and improved glucose tolerance, obesity and insulin resistance in Hf-fed mice by changing colonic microbiota composition. Significance and Impact of the Study: Orally administration of Bacillus licheniformis may reduce body weight and decrease fat deposition by modulating colonic bacterial community in Hf model.

show abstract

“…Particularly, the gastrointestinal microbiome plays multiple roles in host nutrition and gut health (Barko et al ). It has been reported that probiotic supplementation can maintain gut normal microbial composition, and has multiple beneficial effects in livestock species, such as improved growth, intestinal morphology and nutrient digestibility, and inhibited potential pathogens and diarrhoea incidence (Zhang et al ; Pan et al ; Cao et al ). However, the benefit of probiotic as growth promoter and therapeutic antimicrobial is highly variable in weaned piglets.…”

Section: Discussionmentioning

confidence: 99%

Isolation of swine‐derived Lactobacillus plantarum and its synergistic antimicrobial and health‐promoting properties with ZnO nanoparticles

Zheng

et al. 2020

J Appl Microbiol

View full text Add to dashboard Cite

Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy postweaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Of the 128 isolates, Lactobacillus plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. Lactobacillus plantarum BLPL03 was sensitive to β‐lactams, macrolides, amphenicols and cephalosporins, whereas it displayed the steady resistance to aminoglycosides, tetracyclines, quinolones and peptide antibiotics. In vitro analysis of antibacterial activities showed that L. plantarum BLPL03 inhibited the four common food‐borne pathogenic bacteria including Escherichia coli O157:H7 CMCC 44828, Salmonella Typhimurium ATCC 13311, Staphylococcus aureus CMCC 26003 and Listeria monocytogenes CMCC 54007 in synergy with nZnO. Furthermore, the quantitative polymerase chain reaction test demonstrated that the combined administration of L. plantarum BLPL03 fermentation liquor (LFL) and nZnO synergistically elevated the faecal number of Bifidobacterium by 73·19‐fold, and reduced the two potential enteropathogenic bacteria Enterobacteriaceae and Clostridium perfringens in mice challenged with Salm. Typhimurium. Finally, dietary supplementation with low dose of nZnO (20 mg kg−1) when combined with LFL administration enhanced final body weight, fur appearance and average daily gain, and decreased feed conversion ratio and diarrhoea incidence in weaned piglets. The faecal Bifidobacterium and Lactobacillus of piglets were dramatically enhanced by 81·96‐ and 3·15‐fold, respectively, after administration of a mixture of nZnO and LFL. Meanwhile, combination of nZnO with LFL resulted in low levels of Bacteroides, Enterococcus, and Enterobacteriaceae. Conclusions A combination of nZnO and LFL exhibits potential health‐benefit properties for the control of gut microbial composition by their synergistic antimicrobial and probiotic effects. Significance and Impact of the Study This study may provide a potential nutritional strategy to improve performance and gut health of animals with gut microbiota disorders caused by pathogen infections and weanling, and so on.

show abstract

Positive effects of a Clostridium butyricum-based compound probiotic on growth performance, immune responses, intestinal morphology, hypothalamic neurotransmitters, and colonic microbiota in weaned piglets

Abstract: Weaning stress in piglets can lead to poor health outcomes and reduced production.

Cited by 77 publications

References 44 publications

Effects of nucleotides administration on growth performance and immune response of post-weaning piglets

Effects of nucleotides administration on growth performance and immune response of post-weaning piglets

Bacillus licheniformis , a potential probiotic, inhibits obesity by modulating colonic microflora in C57BL/6J mice model

Isolation of swine‐derived Lactobacillus plantarum and its synergistic antimicrobial and health‐promoting properties with ZnO nanoparticles

Contact Info

Product

Resources

About