Isolation of swine‐derived
            <i>Lactobacillus plantarum</i>
            and its synergistic antimicrobial and health‐promoting properties with ZnO nanoparticles

Zheng, Liufeng; Hu, Yangtao; He, Xingping; Zhao, Yu; Xu, Hengyi

doi:10.1111/jam.14605

Cited by 10 publications

(7 citation statements)

References 37 publications

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“…Bacterial synergic effects in the intestinal microflora are well-known and could also play a role in enhancing the preventive effect against weaned piglets' diarrhea. In the study of Zheng et al ( 124 ) the probiotic species L. plantarum have been introduced together with ZnNPs in pigs' diet after weaning. L. plantarum is also known as Zn tolerable bacteria ( 128 ) what was clearly visible from the results.…”

Section: Weaning Of Piglets and Diarrhea Occurrencementioning

confidence: 99%

Importance of Zinc Nanoparticles for the Intestinal Microbiome of Weaned Piglets

et al. 2022

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The scientific community is closely monitoring the replacement of antibiotics with doses of ZnO in weaned piglets. Since 2022, the use of zinc in medical doses has been banned in the European Union. Therefore, pig farmers are looking for other solutions. Some studies have suggested that zinc nanoparticles might replace ZnO for the prevention of diarrhea in weaning piglets. Like ZnO, zinc nanoparticles are effective against pathogenic microorganisms, e.g., Enterobacteriaceae family in vitro and in vivo. However, the effect on probiotic Lactobacillaceae appears to differ for ZnO and zinc nanoparticles. While ZnO increases their numbers, zinc nanoparticles act in the opposite way. These phenomena have been also confirmed by in vitro studies that reported a strong antimicrobial effect of zinc nanoparticles against Lactobacillales order. Contradictory evidence makes this topic still controversial, however. In addition, zinc nanoparticles vary in their morphology and properties based on the method of their synthesis. This makes it difficult to understand the effect of zinc nanoparticles on the intestinal microbiome. This review is aimed at clarifying many circumstances that may affect the action of nanoparticles on the weaning piglets' microbiome, including a comprehensive overview of the zinc nanoparticles in vitro effects on bacterial species occurring in the digestive tract of weaned piglets.

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Section: Weaning Of Piglets and Diarrhea Occurrencementioning

confidence: 99%

Importance of Zinc Nanoparticles for the Intestinal Microbiome of Weaned Piglets

et al. 2022

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“…punctate) [26], Staphylococcus aureus (S. aureus) [27], Listeria monocytogenes (L. monocytogenes) [2] and Clostridium perfringens (C. perfringens) [28]. The fluctuating size of the inhibition zone against pathogens indicates the inhibitory activities of different P. pentosaceus isolates against both gram-negative and gram-positive pathogenic bacteria (Fig.…”

Section: Screening Of Candidate Probiotic P Pentosaceus Strainsmentioning

confidence: 99%

Antioxidant Potential of Pediococcus Pentosaceus Strains from the Sow Milk Bacterial Collection in Weaned Piglets

Wang

Liu

Chen

et al. 2021

Preprint

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Background: In modern animal husbandry, breeders pay increasing attention to improving sow nutrition during pregnancy and lactation to favor the health of neonates. Sow milk is a main food for piglets during their first three weeks of life, which is not only a rich repository of essential nutrients and a broad range of bioactive compounds, but also an indispensable source of commensal bacteria. Maternal milk microorganisms are important sources of commensal bacteria for neonatal gut. Bacteria from maternal milk may serve as an additive to confer a health benefit on the composition of the indigenous microbiota of piglets. Methods: The sow milk microbiota was collected using the culturomics methods of Continuous Culture and Interval Sampling, following by the identification of 16S rDNA gene sequences. To screen potential probiotics, the functional evaluation was conducted to assess their antagonistic activity against pathogens in vitro and evaluate their resistance against oxidative stress in damaged Drosophila induced by paraquat. In piglet feeding trial, a total of 54 newborn suckling piglets were chosen from nine sows and randomly assigned to three treatments with different concentration of a candidate strain. Multiple approaches were carried out to verify its antioxidant function including western blotting, enzyme activity analysis, metabolomics and 16S rDNA sequencing. Results: The 1240 isolates were screened out from the sow milk microbiota and grouped into 271 bacterial taxa based on a nonredundant set of 16S rDNA gene sequences. Among 80 Pediococcus isolates, a new Pediococcus pentosaceus strain (SMM914) showed best performance in inhibition ability against swine pathogens and in Drosophila model challenged by paraquat. Pretreatment of piglets with SMM914 induces the Nrf2-Keap1 antioxidant signaling pathway and greatly affected the pathways of amino acid metabolism and lipid metabolism in plasma. In colon, Lactobacillus was significantly increased in the high dose of SMM914 group compared with the control group. Conclusion: SMM914 functions as a promising probiotic conferring antioxidant capacity by activating the Nrf2-Keap1 antioxidant signaling pathway in piglets. Our study provided useful resources for deeply understanding the relationships between the maternal microbiota and offspring.

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“…Antagonistic activity against pathogens to control their spread is a prerequisite for a potential probiotic [20]. Piglets commonly encounter pathogens on farms at increasing frequencies [21], including Salmonella typhimurium (S. typhimurium) [22], enterohemorrhagic Escherichia coli (EHEC) [23], enterotoxigenic Escherichia coli (ETEC) [24], Klebsiella pneumoniae (K. pneumoniae) [25], Aeromonas punctate (A. punctate) [26], Staphylococcus aureus (S. aureus) [27], Listeria monocytogenes (L. monocytogenes) [2] and Clostridium perfringens (C. perfringens) [28]. The uctuating size of the inhibition zone against pathogens indicates the inhibitory activities of different P. pentosaceus against both gram-negative and gram-positive pathogenic bacteria (Fig.…”

Section: Screening Of Candidate Probiotic P Pentosaceus Strainsmentioning

confidence: 99%

Antioxidant Potential of Pediococcus Pentosaceus Strains From the Sow Milk Bacterial Collection in Weaned Piglets

Wang

Liu

Zhou

et al. 2020

Preprint

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Background: In modern animal husbandry, the relationship between sow and piglets is closely linked as breeders pay more attention to improving sow nutrition during pregnancy and lactation to favor the growing of neonates. Sow milk is the main food for piglets during their first three weeks of life, which is not only a rich repository of essential nutrients and a broad range of bioactive compounds, but also an indispensable source of commensal bacteria. Maternal milk microorganisms are important sources of commensal bacteria for neonatal gut. Bacteria from hosts’ maternal milk may serve as an additive to confer a health benefit on the composition of the indigenous microbiota of piglets.Methods: We first obtained isolates from the sow milk microbiota by the culturomics methods of continuous culture and interval sampling. Then, identified and grouped them based on a nonredundant set of 16S rDNA gene sequences. After assessing their antimicrobial activity against enteropathogens in vitro, we selected several strains to further conduct assay in Drosophila to evaluate their resistance against oxidative injury. We finally screened out Pediococcus pentosaceus (P. pentosaceus) SMM914 as candidate strain to verify its antioxidant effect in weaned piglets and study its probiotic function by 16S rDNA sequencing, metabolomics, western blotting and enzyme activity analysis.Results: The 1240 isolates were screened out from the sow milk microbiota and grouped into 271 bacterial taxa. We evaluated 80 Pediococcus isolates about their inhibition ability against enteropathogens in vitro and then chose top 10 isolates to further test them in Drosophila. In 80 Pediococcus isolates, P. pentosaceus SMM914 showed best performance by inhibition ability against enteropathogens in vitro andparaquat challenge in Drosophila model. Pretreatment of piglets with SMM914 induces the Nrf2/Keap1 antioxidant signaling pathway and altered the pathways of amino acid metabolism and lipid metabolism in plasma. In colon, Lactobacillus was significantly increased in the high dose of SMM914 group compared with the control group.Conclusion: Our study provided useful resources for deeply understanding the relationships between the maternal microbiota and the offspring and supported the theory of Sow and Piglet Integration.

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Isolation of swine‐derived Lactobacillus plantarum and its synergistic antimicrobial and health‐promoting properties with ZnO nanoparticles

Cited by 10 publications

References 37 publications

Importance of Zinc Nanoparticles for the Intestinal Microbiome of Weaned Piglets

Importance of Zinc Nanoparticles for the Intestinal Microbiome of Weaned Piglets

Antioxidant Potential of Pediococcus Pentosaceus Strains from the Sow Milk Bacterial Collection in Weaned Piglets

Antioxidant Potential of Pediococcus Pentosaceus Strains From the Sow Milk Bacterial Collection in Weaned Piglets

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