Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea

Lim, Jee-Min; Jeon, Che Ok; Song, Sung Min; Kim, Chang‐Jin

doi:10.1099/ijs.0.63315-0

Cited by 69 publications

(64 citation statements)

References 25 publications

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“…Flagella were stained according to the method of Leifson (Smibert & Krieg, 1981). Growth was tested at various temperatures (5-55 u C, in increments of 5 u C) on MA5 and at different (Lim et al, 2005), and on some other media as controls [MA, trypticase soy agar (Difco) and ISP medium 2 agar (Shirling & Gottlieb, 1966)]. Aesculin hydrolysis, nitrate reduction and H 2 S and indole production were determined as recommended by Smibert & Krieg (1994).…”

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Saccharospirillum salsuginis sp. nov., a gammaproteobacterium from a subterranean brine

Chen

Cui

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel Gram-negative, non-sporulating, moderately halophilic, facultatively alkaliphilic, catalaseand oxidase-positive, obligately aerobic bacterium, strain YIM-Y25 T , was isolated from a subterranean brine sample collected from a salt mine in Yunnan, south-west China. Cells were spirilla, motile by monopolar flagella, with meso-diaminopimelic acid in the cell-wall peptidoglycan. Growth occurred with 1-15 % (w/v) NaCl (optimum 5 %), at pH 6.0-10.0 (optimum pH 8.0) and at 15-50 6C (optimum 35-40 6C). Ubiquinone Q-8 was the predominant respiratory quinone. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylamine and an unidentified phospholipid. The major cellular fatty acids were C 18 : 1 v7c, iso-C 16 : 0 , C 16 : 0 and C 16 : 1 . The genomic DNA G+C content was 58.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-Y25 T was most closely related to the type strain of the sole recognized species of the genus Saccharospirillum, Saccharospirillum impatiens EL-105 T (sequence similarity 97.0 %), and these two strains formed a robust lineage in the phylogenetic tree. The level of DNA-DNA relatedness between them was 12.6 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic properties and DNA-DNA hybridization data supported the view that this strain represents a novel species of the genus Saccharospirillum, for which the name Saccharospirillum salsuginis sp. nov. is proposed, with YIM-Y25 T (5CCTCC AA 207033 T 5KCTC 22169 T ) as the type strain.The genus Saccharospirillum was proposed by Labrenz et al. (2003) with Saccharospirillum impatiens as the sole recognized species. The genus was defined as Gramnegative, motile by monopolar flagella, non-sporulating, catalase-and oxidase-positive, aerobic-microaerophilic spirilla, with ubiquinone Q-8 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmonomethylamine as the major polar lipids. In a recent study of the microbial diversity in the ancient salt deposit of the Yipinglang salt mine (25 u 189 N 101 u 549 E) in Yunnan, south-west China (Chen et al., 2007a, b), a moderately halophilic, facultatively alkaliphilic strain, YIM-Y25 T , was isolated from a subterranean brine sample. The results of our polyphasic taxonomic study indicated that this strain represents a novel species of the genus Saccharospirillum.Strain YIM-Y25 T was isolated from a subterranean brine sample by plating 1 : 10 serial dilutions of the sample on Difco marine agar 2216 (MA) supplemented with 3 % (w/v) NaCl [containing approximately 5 % (w/v) NaCl in total; MA5] at 37 u C for 2 weeks. After primary isolation and purification, the isolate was kept as serial transfer cultures on MA5 slants, lyophilized cultures at 4 u C or deep-frozen cultures at 280 u C in Difco marine broth 2216 (MB) supplemented with 20 % (v/v) glycerol. The reference strain S. impatiens DSM 12546 T was obtained from the DSMZ....

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Saccharospirillum salsuginis sp. nov., a gammaproteobacterium from a subterranean brine

Chen

Cui

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…The genus Pontibacillus was first proposed by Lim et al (2005a). Members of this genus are Gram-positive, moderately halophilic, strictly aerobic, motile, endosporeforming rods, having a meso-diaminopimelic acid cell-wall type and containing MK-7 as the predominant respiratory quinone.…”

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“…Members of this genus are Gram-positive, moderately halophilic, strictly aerobic, motile, endosporeforming rods, having a meso-diaminopimelic acid cell-wall type and containing MK-7 as the predominant respiratory quinone. At the time of writing, the genus comprises two recognized species, Pontibacillus chungwhensis (Lim et al, 2005a) and Pontibacillus marinus (Lim et al, 2005b).During an investigation of the diversity of the microbial population of invertebrates inhabiting the South China Sea, we isolated a moderately halophilic bacterium, designated JSM 076056 T , from a sea urchin collected from Leizhou Bay, China. On the basis of the results of a polyphasic taxonomic study, this strain represents a novel species of the genus Pontibacillus.…”

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Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin

Chen

Zhang

Xiao

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-positive-staining, moderately halophilic, strictly aerobic, catalase-and oxidase-positive, rod-shaped bacterium, designated strain JSM 076056 T , was isolated from a sea urchin collected from the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 076056 T was able to grow at salinities of 2-25 % (w/v) total salts and at pH 6.0-10.0 and 15-40 6C; optimum growth was observed with 5-10 % (w/v) total salts and at pH 7.0-8.0 and 25-30 6C. It was incapable of growing with NaCl as the sole salt. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C 15 : 0 , iso-C 16 : 0 , iso-C 14 : 0, anteiso-C 15 : 0 and C 16 : 1 v7c alcohol. The predominant respiratory quinone was MK-7 and the genomic DNA G+C content was 45.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076056 T belonged to the family Bacillaceae and was related most closely to the type strains of the two recognized species of the genus Pontibacillus, namely Pontibacillus chungwhensis BH030062 T (96.4 % sequence similarity) and Pontibacillus marinus BH030004 T (96.2 %); these three strains formed a robust cluster in the phylogenetic tree. In combination, the phylogenetic, phenotypic and chemotaxonomic data indicate that strain JSM 076056 T represents a novel species of the genus Pontibacillus, for which the name Pontibacillus halophilus sp. nov. is proposed. The type strain is JSM 076056 T (5CCTCC AA 207029 T 5DSM 19796 T 5KCTC 13190 T ).The genus Pontibacillus was first proposed by Lim et al. (2005a). Members of this genus are Gram-positive, moderately halophilic, strictly aerobic, motile, endosporeforming rods, having a meso-diaminopimelic acid cell-wall type and containing MK-7 as the predominant respiratory quinone. At the time of writing, the genus comprises two recognized species, Pontibacillus chungwhensis (Lim et al., 2005a) and Pontibacillus marinus (Lim et al., 2005b).During an investigation of the diversity of the microbial population of invertebrates inhabiting the South China Sea, we isolated a moderately halophilic bacterium, designated JSM 076056 T , from a sea urchin collected from Leizhou Bay, China. On the basis of the results of a polyphasic taxonomic study, this strain represents a novel species of the genus Pontibacillus.For strain isolation, serial dilutions (1 : 10) of a sea urchin homogenate were plated on Difco marine agar 2216 (MA; pH 7.5), supplemented with 0-20 % (w/v) NaCl, at 28 u C for 7-28 days. A yellow-pigmented colony, designated strain JSM 076056 T , was picked from a plate of MA supplemented with 10 % (w/v) NaCl. It was maintained both on slants of MA supplemented with 5 % (w/v) NaCl (containing approximately 7 % NaCl and 8.4 % total salts; here referred to as MA5) at 4 u C and in Difco marine broth 2216 (MB) supplemented with 20 % (v/v) glycerol at 280 u C. Unless indicated otherwise, morphological and physiological studi...

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“…Moderately halophilic bacteria that grow optimally in media containing 3-15 % (w/v) NaCl are widely distributed in different marine environments and are made up of very heterogeneous physiological groups taxonomically, including both Gram-positive and Gram-negative microorganisms (Ventosa et al, 1998;Oren, 2002;Jeon et al, 2005a;Lim et al, 2005). Aerobic, spore-forming, halophilic, Gram-positive rods that were originally assigned to the genus Bacillus are also very diverse taxonomically and they form several phylogenetically distinct lineages (Ash et al, 1991;Stackebrandt & Liesack, 1993;Nielsen et al, 1994;Jeon et al, 2005b).…”

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Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China

Jeon

Lim

Jang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Two Gram-positive, motile, rod-shaped, halophilic bacteria showing optimum growth at 5-7 % (w/v) NaCl were isolated from a salt lake, Xin-Jiang Province, China. The strains, designated BH312 T and BH314, grew in the presence of 1-18 % (w/v) NaCl and at temperatures of 15-50 6C (optimum: 40 6C) and pH values of 5.5-10.0 (optimum: pH 7.5-8.0). The major cellular fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and anteiso-C 17 : 0 . The DNA G+C content was 38.8-39.0 mol% and the predominant lipoquinone was MK-7. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates formed a cluster with Gracilibacillus orientalis XH-63 T within the genus Gracilibacillus. The levels of 16S rRNA gene sequence similarity with closely related type strains were lower than 96.2 %. On the basis of phylogenetic, phenotypic and chemotaxonomic properties, the isolates represent a novel species of the genus Gracilibacillus for which the name Gracilibacillus lacisalsi sp. nov. is proposed. The type strain is BH312 T (5KCTC 13129 T 5DSM 19029 T ).Moderately halophilic bacteria that grow optimally in media containing 3-15 % (w/v) NaCl are widely distributed in different marine environments and are made up of very heterogeneous physiological groups taxonomically, including both Gram-positive and Gram-negative microorganisms (Ventosa et al., 1998;Oren, 2002;Jeon et al., 2005a;Lim et al., 2005). Aerobic, spore-forming, halophilic, Gram-positive rods that were originally assigned to the genus Bacillus are also very diverse taxonomically and they form several phylogenetically distinct lineages (Ash et al., 1991;Stackebrandt & Liesack, 1993;Nielsen et al., 1994;Jeon et al., 2005b). Among them, the genus Gracilibacillus was first proposed by Wainø et al. (1999) to accommodate Gracilibacillus halotolerans, isolated from the Great Salt Lake, Utah (USA), and Gracilibacillus dipsosauri, transferred from Bacillus dipsosauri. Currently, the genus includes only four species: G. halotolerans, G. dipsosauri (Wainø et al., 1999), G. orientalis (Carrasco et al., 2006) and G. boraciitolerans (Ahmed et al., 2007). In the course of screening sediment of Ai-Ding Lake, Xin-Jiang Province, China, to isolate halophilic bacteria, two aerobic, Gram-positive, moderately halophilic bacteria, strains BH312 T and BH314, were isolated. Ai-Ding Lake (42 u 329 100-42 u 499 130 N 89 u 109 320-89 u 549 320 E) is located in the most arid area of China, where evaporation is significantly higher than precipitation. The lake is the lowest point in China (154 m below sea-level) and has been a target area for the study of halophilic microorganisms for many years. Water is largely supplied from the nearby glacier of Tian-Shan Mountain. Ai-Ding Lake is a typical chloride-sulfate saline lake with a slightly alkaline pH (7.2-7.6) and a salt concentration of 20-26 % (w/v). The content of Mg 2+ and Ca 2+ in the brine is very low. On the basis of phy...

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Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea

Cited by 69 publications

References 25 publications

Saccharospirillum salsuginis sp. nov., a gammaproteobacterium from a subterranean brine

Saccharospirillum salsuginis sp. nov., a gammaproteobacterium from a subterranean brine

Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin

Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China

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