Saccharospirillum salsuginis sp. nov., a gammaproteobacterium from a subterranean brine

Abstract

A novel Gram-negative, non-sporulating, moderately halophilic, facultatively alkaliphilic, catalaseand oxidase-positive, obligately aerobic bacterium, strain YIM-Y25 T , was isolated from a subterranean brine sample collected from a salt mine in Yunnan, south-west China. Cells were spirilla, motile by monopolar flagella, with meso-diaminopimelic acid in the cell-wall peptidoglycan. Growth occurred with 1-15 % (w/v) NaCl (optimum 5 %), at pH 6.0-10.0 (optimum pH 8.0) and at 15-50 6C (optimum 35-40 6C). Ubiquinone Q-8 was the predominant respiratory quinone. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylamine and an unidentified phospholipid. The major cellular fatty acids were C 18 : 1 v7c, iso-C 16 : 0 , C 16 : 0 and C 16 : 1 . The genomic DNA G+C content was 58.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-Y25 T was most closely related to the type strain of the sole recognized species of the genus Saccharospirillum, Saccharospirillum impatiens EL-105 T (sequence similarity 97.0 %), and these two strains formed a robust lineage in the phylogenetic tree. The level of DNA-DNA relatedness between them was 12.6 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic properties and DNA-DNA hybridization data supported the view that this strain represents a novel species of the genus Saccharospirillum, for which the name Saccharospirillum salsuginis sp. nov. is proposed, with YIM-Y25 T (5CCTCC AA 207033 T 5KCTC 22169 T ) as the type strain.The genus Saccharospirillum was proposed by Labrenz et al. (2003) with Saccharospirillum impatiens as the sole recognized species. The genus was defined as Gramnegative, motile by monopolar flagella, non-sporulating, catalase-and oxidase-positive, aerobic-microaerophilic spirilla, with ubiquinone Q-8 as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmonomethylamine as the major polar lipids. In a recent study of the microbial diversity in the ancient salt deposit of the Yipinglang salt mine (25 u 189 N 101 u 549 E) in Yunnan, south-west China (Chen et al., 2007a, b), a moderately halophilic, facultatively alkaliphilic strain, YIM-Y25 T , was isolated from a subterranean brine sample. The results of our polyphasic taxonomic study indicated that this strain represents a novel species of the genus Saccharospirillum.Strain YIM-Y25 T was isolated from a subterranean brine sample by plating 1 : 10 serial dilutions of the sample on Difco marine agar 2216 (MA) supplemented with 3 % (w/v) NaCl [containing approximately 5 % (w/v) NaCl in total; MA5] at 37 u C for 2 weeks. After primary isolation and purification, the isolate was kept as serial transfer cultures on MA5 slants, lyophilized cultures at 4 u C or deep-frozen cultures at 280 u C in Difco marine broth 2216 (MB) supplemented with 20 % (v/v) glycerol. The reference strain S. impatiens DSM 12546 T was obtained from the DSMZ....

Comparative molecular analysis of the prokaryotic diversity of two salt mine soils in southwest China

“…Seventy-four percent of QH bacterial clones and 89% of YPL bacterial clones had their greatest (87-100%) sequence identity with uncultured environmental clones. The diversity of novel phylogenetic groups in these salt mines is supported by previous work that yielded several novel bacterial isolates [21][22][23][24][25][26].…”

Saccharospirillum aestuarii sp. nov., isolated from tidal flat sediment, and an emended description of the genus Saccharospirillum

“…T was similar to those of S. impatiens and S. salsuginis (Labrenz et al, 2003;Chen et al, 2009) except for the presence of lyso-phosphatidylethanolamine. The cellular fatty acid profile of strain IMCC4453…”

“…The results of the phenotypic characterization of S. salsuginis YIM-Y25 T and S. impatiens DSM 12546 T observed in this study that were different from the original descriptions (Labrenz et al, 2003;Chen et al, 2009) are shown in Supplementary Table S1. These discrepancies might be due to the different incubation temperature and different suspension media used for the API ZYM, API 50CH and Biolog GN2 tests.…”

“…The genus Saccharospirillum (Labrenz et al, 2003) belongs to the order Oceanospirillales (Garrity et al, 2005) and, at the time of writing, comprises two recognized species, Saccharospirillum impatiens (Labrenz et al, 2003) and Saccharospirillum salsuginis (Chen et al, 2009). The genus accommodates Gram-negative, chemoheterotrophic, aerobic, motile by monopolar flagella, spirillum-shaped bacteria, which have Q-8 as the major respiratory quinone.…”

First insight on interactions between bacteria and the marine diatom Haslea ostrearia: Algal growth and metabolomic fingerprinting

“…The Saccharospirillum genus belongs to the order Oceanospirillum [45,46]. Currently, this genus includes three species: S. impatientis [45] and S. salsuginis [47] were isolated from hypersaline environments, and S. aesturii was isolated from a tidal flat sediment [48].…”