First insight on interactions between bacteria and the marine diatom Haslea ostrearia: Algal growth and metabolomic fingerprinting

Lepinay, Alexandra; Turpin, Vincent; Mondeguer, Florence; Grandet-Marchant, Quentin; Capiaux, Hervé; Baron, Régis; Lebeau, Thierry

doi:10.1016/j.algal.2018.02.023

Cited by 23 publications

(17 citation statements)

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“…Although an extensive literature exists on algalbacterial interactions and their impacts on algal physiology, few studies have documented differences in intracellular metabolites (Paul et al 2012, Chen et al 2017) and extracellular metabolites (L epinay et al 2018(L epinay et al , Piampiano et al 2020) with untargeted analyses. The finding that most of the exometabolites with a treatment effect were in lower concentrations in some or all of the co-cultures compared to the axenic culture suggests that the bacteria likely incorporated some of these compounds for growth and energy, as heterotrophic bacteria are known to do in natural aquatic ecosystems (Azam et al 1983).…”

Section: Resultsmentioning

confidence: 99%

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The Influence of Bacteria on the Growth, Lipid Production, and Extracellular Metabolite Accumulation by Phaeodactylum tricornutum (Bacillariophyceae)

Chorazyczewski

Huang

Abdulla

et al. 2021

Journal of Phycology

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To examine the impact of heterotrophic bacteria on microalgal physiology, we co‐cultured the diatom Phaeodactylum tricornutum with six bacterial strains to quantify bacteria‐mediated differences in algal biomass, total intracellular lipids, and for a subset, extracellular metabolite accumulation. A Marinobacter isolate significantly increased algal cell concentrations, dry biomass, and lipid content compared to axenic algal cultures. Two other bacterial strains from the Bacteroidetes order, of the genera Algoriphagus and Muricauda, significantly lowered P. tricornutum biomass, leading to overall decreased lipid accumulation. These three bacterial co‐cultures (one mutualistic, two competitive) were analyzed for extracellular metabolites via untargeted liquid chromatography mass spectrometry to compare against bacteria‐free cultures. Over 80% of the extracellular metabolites differentially abundant in at least one treatment were in higher concentrations in the axenic cultures, in agreement with the hypothesis that the co‐cultured bacteria incorporated algal‐derived organic compounds for growth. Furthermore, the extracellular metabolite profiles of the two growth‐inhibiting cultures were more similar to one another than the growth‐promoting co‐culture, linking metabolite patterns to ecological role. Our results show that algal–bacterial interactions can influence the accumulation of intracellular lipids and extracellular metabolites, and suggest that utilization and accumulation of compounds outside the cell play a role in regulating microbial interactions.

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Section: Resultsmentioning

confidence: 99%

“…2017) and extracellular metabolites (Lépinay et al. 2018, Piampiano et al. 2020) with untargeted analyses.…”

Section: Resultsmentioning

confidence: 99%

The Influence of Bacteria on the Growth, Lipid Production, and Extracellular Metabolite Accumulation by Phaeodactylum tricornutum (Bacillariophyceae)

Chorazyczewski

Huang

Abdulla

et al. 2021

Journal of Phycology

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“…F10, and A. macleodii F12 from the natural diatom microbial consortium provides an ample prospect to better understand phytoplankton modulation of different bacterial taxa in the phycosphere. Sulfitobacter and Phaeobacter are considered beneficial bacteria to diatoms, dinoflagellates, and macroalgae as they provide a variety of essential nutrients and cofactors that confer metabolic advantage to their hosts ( 17 , 18 , 51 – 56 ). Remarkably, several S. pseudonitzschiae strains (16S rRNA gene sequence identity >97%) have been isolated from several diatom species originating from different oceanic regions ( 18 , 57 , 58 ).…”

Section: Discussionmentioning

confidence: 99%

Diatom modulation of select bacteria through use of two unique secondary metabolites

Shibl

Isaac

Ochsenkühn

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

140

112

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Unicellular eukaryotic phytoplankton, such as diatoms, rely on microbial communities for survival despite lacking specialized compartments to house microbiomes (e.g., animal gut). Microbial communities have been widely shown to benefit from diatom excretions that accumulate within the microenvironment surrounding phytoplankton cells, known as the phycosphere. However, mechanisms that enable diatoms and other unicellular eukaryotes to nurture specific microbiomes by fostering beneficial bacteria and repelling harmful ones are mostly unknown. We hypothesized that diatom exudates may tune microbial communities and employed an integrated multiomics approach using the ubiquitous diatom Asterionellopsis glacialis to reveal how it modulates its naturally associated bacteria. We show that A. glacialis reprograms its transcriptional and metabolic profiles in response to bacteria to secrete a suite of central metabolites and two unusual secondary metabolites, rosmarinic acid and azelaic acid. While central metabolites are utilized by potential bacterial symbionts and opportunists alike, rosmarinic acid promotes attachment of beneficial bacteria to the diatom and simultaneously suppresses the attachment of opportunists. Similarly, azelaic acid enhances growth of beneficial bacteria while simultaneously inhibiting growth of opportunistic ones. We further show that the bacterial response to azelaic acid is numerically rare but globally distributed in the world’s oceans and taxonomically restricted to a handful of bacterial genera. Our results demonstrate the innate ability of an important unicellular eukaryotic group to modulate select bacteria in their microbial consortia, similar to higher eukaryotes, using unique secondary metabolites that regulate bacterial growth and behavior inversely across different bacterial populations.

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“…Interestingly, a functional carbon flux between diatoms and bacteria has been observed since some bacterial species have been found as intermediate providers for the biosynthesis of bioactive metabolites [ 130 ]. Overall, co-occurring bacteria promote the growth of diatom cells, influencing their metabolism and improving their biotechnological potential [ 131 , 132 , 133 , 134 , 135 , 136 ].…”

Section: Biotechnological Applications Of Oxylipinsmentioning

confidence: 99%

Multiple Roles of Diatom-Derived Oxylipins within Marine Environments and Their Potential Biotechnological Applications

et al. 2020

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The chemical ecology of marine diatoms has been the subject of several studies in the last decades, due to the discovery of oxylipins with multiple simultaneous functions including roles in chemical defence (antipredator, allelopathic and antibacterial compounds) and/or cell-to-cell signalling. Diatoms represent a fundamental compartment of marine ecosystems because they contribute to about 45% of global primary production even if they represent only 1% of the Earth’s photosynthetic biomass. The discovery that they produce several toxic metabolites deriving from the oxidation of polyunsaturated fatty acids, known as oxylipins, has changed our perspectives about secondary metabolites shaping plant–plant and plant–animal interactions in the oceans. More recently, their possible biotechnological potential has been evaluated, with promising results on their potential as anticancer compounds. Here, we focus on some recent findings in this field obtained in the last decade, investigating the role of diatom oxylipins in cell-to-cell communication and their negative impact on marine biota. Moreover, we also explore and discuss the possible biotechnological applications of diatom oxylipins.

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First insight on interactions between bacteria and the marine diatom Haslea ostrearia: Algal growth and metabolomic fingerprinting

Abstract: Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site.

Cited by 23 publications

References 55 publications

The Influence of Bacteria on the Growth, Lipid Production, and Extracellular Metabolite Accumulation by Phaeodactylum tricornutum (Bacillariophyceae)

The Influence of Bacteria on the Growth, Lipid Production, and Extracellular Metabolite Accumulation by Phaeodactylum tricornutum (Bacillariophyceae)

Diatom modulation of select bacteria through use of two unique secondary metabolites

Multiple Roles of Diatom-Derived Oxylipins within Marine Environments and Their Potential Biotechnological Applications

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