Polymorphism of D-Loop Mitochondrial DNA Region and Phylogenetic in Five Indonesian Native Duck Population

Purwantini, Dattadewi; Yuwanta, Tri; Hartatik, Tety; Ismoyowati, .

doi:10.3923/ijps.2013.55.63

Cited by 14 publications

(16 citation statements)

References 21 publications

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“…The genetic distance between Tegal and Magelang duck population closer than genetic distance between Gallang and Maggal duck (Table 4). The genetic distance between Tegal and Magelang duck in this study according to the previous study was reported by Purwantini et al (2013a) about 0.009-0, 691 based on D-loop mtDNA region. Gallang and Maggal ducks as the offspring of reciprocal crosses has a similarity and relationship tend to be closer to Tegal ducks compared with Magelang ducks.…”

Section: Polymorphisms Of Cytochrome B and Genetic Distancementioning

confidence: 96%

“…South-East Asian duck population were closely related to the mallard duck (Anas platyrhynchos) that indicated by their mean overall genetic distance. Indonesian domestic ducks has a relatively closed genetic relationship with Anas Platyrhynchos and Anas zonorhyncha as shown by the variation of the genetic distance ranged from 0.000 to 0.786 (Purwantini et al, 2013a).…”

Section: Introductionmentioning

confidence: 99%

“…Tegal ducks and Magelang ducks had the genetic distance ranged from 0.009 to 0.691 based on the Single Nucleotide Polymorphism (SNP) analysis on the D-loop mtDNA region (Purwantini et al, 2013a). Nevertheless, information about the genetic diversity of Tegal, Magelang, and their crossbred by PCR-RFLP based on polymorphism of cyt b mtDNA has still limited.…”

Section: Introductionmentioning

confidence: 99%

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Morphometrics and genetic diversity of Tegal, Magelang and their crossbred ducks based on Cytochrome b gene

Henrik¹,

Purwantini

Ismoyowati

2018

J. Indonesian Trop. Anim. Agric.

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This experiment was conducted to study the morphometric and genetic diversity of Tegal, Magelang, and their crossbred ducks. Each ten female about twenty weeks old ducks from Tegal, Magelang, Gallang, and Maggal ducks, respectively, were used as the group materialss. The body weight, chest circumference, body length, shank length, neck length, and pubis width were recorded and tested by analysis of variance of one way classification as the morphometric characteristics. Polymorphism of cytochrome b (cyt b) gene on mitochondrial DNA (mtDNA) was analyzed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Genetic distance was analyzed based on value of heterozygosity, whereas the phylogeny tree was reconstructed using MEGA6 software. The results showed there were highly significant difference (P<0.01) on body weight, chest circumference, body length, and neck length between population, while shank and pubis width were not significant different. The genetic distance between Gallang and Maggal ducks (0.206) was higher than Tegal and Magelang ducks (0.169). It is concluded that the reciprocal crosses increased the morphometric and genetic diversity of Indonesian local duck population.

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Section: Polymorphisms Of Cytochrome B and Genetic Distancementioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphometrics and genetic diversity of Tegal, Magelang and their crossbred ducks based on Cytochrome b gene

Henrik¹,

Purwantini

Ismoyowati

2018

J. Indonesian Trop. Anim. Agric.

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“…PCR process was performed with condition predenaturation at 94°C for 5 minutes, denaturation at 94°C for 30 seconds, annealing at 51°C for 45 seconds, elongation (extension) at 72°C for 1 minute and post elongation at 72°C for 5 minutes. PCR was repeated for 35 cycles for optimum result (Purwantini et al, 2013). PCR product was separated by electrophoresis agarose gel 1% using buffer 1x TBE in Submarine Electrophoresis (Hoefer, USA).…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Genetic Diversity of Kejobong Goat Based on Mitochondrial Dna D-Loop Sequence

Harlistyo

Sutopo

Kurnianto

2014

J. Indonesian Trop. Anim. Agric.

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“…Primers used were forward primer DL-AnasPF (L56) 5'-GTTGCGGGGTTATTTGGTTA-3 'and reverse primer DL-AnasPR (H773) 5'-CCA-TATACGCCAACCGTCTC-3' (Purwantini, Yuwanta, & Hartatik, 2013). The PCR program was pre-denaturation 94 °C for 5 minutes, denaturation 94 °C for 30 seconds, annealing 56.1 °C for 45 seconds, extension 72 °C for 1 min, and a final extension 72 °C for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

PCR-RFLP Analysis of D-Loop mtDNA in Indonesian Domestic Waterfowl

2017

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Phylogenetic or population genetic studies have been focused on finding genetic variations in mitochondrial DNA (mtDNA) because mutations in this region contained five times higher than other genetic materials. The aim of this research was to generate PCR-RFLP D-loop mtDNA profiles of some Indonesian domestic waterfowl. The samples were 35 local ducks, 5 muscovy duck, and 5 geese. These local ducks included on seven types of Javanese ducks in Central Java, i.e. Magelang duck, Peking duck, Pengging duck, Tegal Branjangan duck, Tegal Jarakan duck, Tegal Blorong duck, and Tegal Lemahan duck. PCR technique was used to amplify the D-loop genes, and RFLP analysis of the D-loop was performed with AluI and RsaI. The results show that muscovy duck has no restriction sites in D-loop gene. Moreover, goose has a restriction site of AluI in D-loop gene at 550 bp and 187 bp; and it has no restriction site of RsaI. The analysis of RsaI-RFLP in mtDNA D-loop region of ducks resulted in 1 restriction fragment pattern which can be applied in the identification of duck to differentiate it from goose and muscovy duck. PCR-RFLP analysis duck D-loop mtDNA using AluI and RsaI restriction enzyme resulted in 3 combinations of restriction fragment pattern shown in 3 haplotypes (A, B, and C). Genetic diversity of Indonesian domestic waterfowl population could be identified by using PCR-RFLP analysis on mtDNA D-loop region. So far, there are few results of research on Indonesian domestic waterfowl genetic characteristics based on PCR-RFLP mtDNA D-loop marker. Also, this research has a good contribution on genetic characterization of local duck. The characterization is an important aspect to maintain breed quality and manage the genetic resources of Indonesian germplasm and in facilitating the breeding program.

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Polymorphism of D-Loop Mitochondrial DNA Region and Phylogenetic in Five Indonesian Native Duck Population

Cited by 14 publications

References 21 publications

Morphometrics and genetic diversity of Tegal, Magelang and their crossbred ducks based on Cytochrome b gene

Morphometrics and genetic diversity of Tegal, Magelang and their crossbred ducks based on Cytochrome b gene

Genetic Diversity of Kejobong Goat Based on Mitochondrial Dna D-Loop Sequence

PCR-RFLP Analysis of D-Loop mtDNA in Indonesian Domestic Waterfowl

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