Hakikat pembangunan berkelanjutan merupakan peningkatan kualitas hidup manusia dan menjamin keberlanjutannya. Dewasa ini, pembangunan kota hanya menitikberatkan perubahan lingkungan dan tidak membangun manusia yang menempatinya. Bahkan, perkembangan kota-kota Indonesia cenderung mengikuti mekanisme pasar, seperti permukiman di Kelurahan Terboyo Wetan Kecamatan Genuk, Semarang. Permukiman ini berkembang sejak beroperasinya zona industri Genuk pada tahun 1980-an. Awalnya, permukiman ini merupakan perkampungan nelayan yang masyarakatnya menggantungkan hidupnya pada laut. Industrialisasi besar-besaran telah mengubah kondisi kehidupan masyarakat. Untuk menelusuri lebih mendalam, penelitian ini memiliki tiga tujuan, yaitu (1) memahami kesejahteraan masyarakat, (2) memahami preferensi masyarakat terhadap kesejahteraan, dan (3) memahami seberapa besar kemampuan kesejahteraan masyarakat untuk mendukung permukiman yang berkelanjutan. Penelitian ini menggunakan metode kualitatif dan membuktikan hipotesis dengan realitas di lapangan. Temuan studi menunjukkan permukiman Terboyo Wetan rawan tidak berlanjut. Menurut penilaian objektif, dari 5 aspek kualitas hidup menurut pemahaman human settlement hanya satu aspek yang mendukung, yaitu society. Keempat aspek lainnya (man, nature, shells, dan network) tidak mendukungnya. Begitupula cara pandang warga yang bersifat antroposentris berdampak pada perilaku kurang ramah lingkungan. Ini tercermin dari makna kesejahteraan menurut warga, yaitu pemenuhan kebutuhan dasar dalam lingkup ekonomi dan belum memikirkan aspek lingkungan hidup. Bahkan, beberapa warga menganggap kondisi lingkungan yang terpuruk merupakan konsekuensi permukiman pesisir.

Susanti R, Iswari RS, Fibriana F, Indriawati. 2018. The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool. Biodiversitas 19: 997-1003. The local duck DNA barcoding is still rarely conducted in Indonesia while DNA barcoding is extensively used as a tool of species identification and delineation tool. This study aimed to analyze the sequence and patterns of Central Javanese ducks mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Feather samples of seven breeds of native duck were collected from traditional husbandries in Central Java. The samples were employed for DNA extraction and COI gene amplification. Five haplotypes were obtained from 35 samples, i.e., haplotype A, B, C, D, and E. Also, 9 variable sites with synonym substitution was detected in four nucleotides number 55, 61, 100, and 109; whereas five synonym substitutions were identified in the nucleotides number 36, 48, 51, 66, and 756. In conclusion, this study annotates that COI mtDNA gene is essential for local ducks barcoding system.

Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the body's nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, β-carotene, vitamin C, α-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. α-tocopherol, β-carotene and vitamin C) and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.

e127 are commonly associated with neonatal diarrhea and dysentery in calves. It is important to determine minimal inhibitory concentrations(MICs) for the treatment of animals including human with E. coli infections. A total of 160 strains from 158 diarrheic cattle(1 day to 11 years old) were examined between April 1999 and December 2001 in Japan. The isolates were tested about characteristic pathogenic genes (stx 1 , stx 2 , and eaeA) by polymerase chain reaction. Pathogenic E. coli were tested in vitro for their susceptibility against 18 antimicrobial agents [ampicillin(ABPC), amoxicillin(AMPC), penicillin G(PCG), gentamycin(GM), kanamycin(KM), erythromycin(EM), tetracycline(TC), oxytetracycline(OTC), chloramphenicol(CP), bicozamycin(BCM), fosfomycin(FOM), sulfadimethoxine(SMT), oxolinic acid(OXA), nalidixic acid(NA), enrofloxacin(EFLX), norfloxacin(NFLX), colistin(CL) and trimethoprim-sulfamethoxazole(TMP-S)] using the guidelines of the Clinical and Laboratory Standards Institute(CLSI). The results showed that the E. coli strains at MIC 90 were susceptible to EFLX and GM but were resistant to EM, TC, OTC, CP, PCG, AMPC, ABPC and KM. In the case of PCG, the first peak is at the 16-32 g/ml level which is already considered a resistant value but another peak was demonstrated at a much higher MIC (>512 g/ml). The results suggested that E. coli may be still continued to develop stronger resistance.

No abstract

ABSTRACT. This study was realized to determine the genetic variation of Central Javanese duck based on the D-Loop mtDNA gene. D-loop gene was amplified using PCR technique by specific primer and sequenced using dideoxy termination method with ABI automatic sequencer. ClustalW from MEGA-6.06 software program was employed for multiple alignments of nucleotide sequences. Nucleotide sequences of D-loop gene of mtDMA from the Central Javanese duck were aligned together with other Anas isolates from Genbank using ClustalW of MEGA-6.06 program. The estimation of genetic distance and phylogenetic tree construction were analyzed by Neighbor-Joining method, whereas the calculation of distance matrix was performed using Kimura 2-parameter. Multiple alignments obtained were 720 nucleotides at position 56 to 779 at the 5 'end. The results of the polymorphism analysis on D-loop sequences produced 23 haplotypes. However, this haplotype information does not represent the relationship among the geographical origins of duck with the certain duck species name. Moreover, a total number of 32 variable sites were identified. Insertions were detected in four sequences (126, 155, 771 and 779 nucleotide number). In the phylogenetic analysis, it is safe to conclude that the Central Javanese duck is closely related to Anas platyrhynchos and Anas zonorynchos.

Inferior local tubers such as lesser yam, wild yam and arrowroot contain bioactive compounds such as phenols and diosgenin which act as antioxidants. Antioxidant activities in the processed of local tubers have not been reported. The aim of this research was to identify the antioxidant activity of local tubers (inferior) that has been processed through the process of steaming, frying and milling to be flour. The determination of antioxidant activity was performed using α, α, Diphenyl Picryl Hydrazil (DPPH), which is indicated as a percentage of the apprehension of radical activities by the amount of absorbance at 680 nm. The antioxidant activity of lesser yam steamed, fried and milled were 2.26 %, 0.84 %, and 3.34 % respectively. The antioxidant activity of steamed, fried and milled arrowroot were 1.44 %, 0.65 %, and 1.32 % respectively. Moreover, the antioxidant activity of steamed, fried and milled wild yam were 1.08 %, 0.08 %, and 0.82 % respectively. The antioxidant activity of inferior local tubers have decreased in different types of processing. The highest antioxidant activity was in the milled tubers followed by steamed and fried tuber.

Persaingan dalam dunia bisnis makanan kini semakin ketat, maka dari itu para pelaku bisnis makanan dituntut untuk menciptakan strategi yang dapat menarik para konsumen untuk mengkonsumsi produk atau jasa yang ditawarkan agar mampu bersaing dan unggul dibanding pesaingnya. Tujuan penelitian ini adalah menganalisis signifikansi pengaruh kualitas produk terhadap kepuasan konsumen, menganalisis signifikansi pengaruh brand image terhadap kepuasan konsumen, menganalisis signifikansi pengaruh kepuasan konsumen terhadap loyalitas konsumen, menganalisis signifikansi pengaruh kualitas produk terhadap loyalitas konsumen, menganalisis signifikansi pengaruh brand image terhadap loyalitas konsumen, menganalisis signifikansi pengaruh kualitas produk terhadap loyalitas konsumen yang dimediasi oleh kepuasan konsumen, menganalisis signifikansi pengaruh brand image terhadap loyalitas konsumen yang dimediasi oleh kepuasan konsumen. Hasil penelitian menunjukkan kualitas produk berpengaruh signifikan terhadap kepuasan konsumen, brand image berpengaruh signifikan terhadap kepuasan konsumen, kepuasan konsumen berpengaruh signifikan terhadap loyalitas konsumen, kualitas produk berpengaruh signifikan terhadap loyalitas konsumen, brand image berpengaruh tidak signifikan terhadap loyalitas konsumen, kualitas produk berpengaruh signifikan terhadap loyalitas konsumen yang dimediasi oleh kepuasan konsumen dan brand image berpengaruh terhadap loyalitas konsumen yang dimediasi oleh kepuasan konsumen.